IL-4RA gene expression in PBMC with regard to place of living and atopy status

Danielewicz, Hanna; Dębińska, Anna; Drabik-Chamerska, Anna; Kalita, Danuta; Boznański, Andrzej

doi:10.17219/acem/67787

Cited by 2 publications

(5 citation statements)

References 27 publications

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“…Although the results were not statistically significant, they may suggest an environment-related regulatory mechanism connected with rural living. 13 The aim of the current study was to determine whether the most important and well-studied SNPs within the IL4RA gene are responsible for the different patterns of gene expression. We observed no significant differences in expression profile according to genotype within the different models.…”

Section: Discussionmentioning

confidence: 99%

“…The methods for relative gene expression using realtime quantitative polymerase chain reaction RT-qPCR) and the characteristics of the study group have already been described in detail. 13 We used the expression data from the subjects enrolled in our previous study and performed genotyping for ILR4A polymorphisms. In the current analysis, 36 subjects with available data were enrolled, 18 of whom were atopic.…”

Section: Methodsmentioning

confidence: 99%

“…ACTB (β-actin) was used as reference. 13 Delta Ct (ΔCT) and delta-delta mean Ct (ΔΔCT) values were used for relative quantification of IL4RA expression in PBMC, and 2^-ΔΔCT was used for fold change (FC) estimation in groups according to genotype. Genotyping for specific SNPs, including rs1805010 (I50V), rs1801275 (Q551R) and rs2057768 (C-3223T), was performed using specific Light SNP primers (TIB Molbiol, Berlin, Germany) and the Light-Cycler 1.5.…”

Section: Methodsmentioning

confidence: 99%

“…We did find a trend for atopic subjects to have a higher expression and for those living in the countryside to have lower values, though the differences were not statistically significant. 13 Herein, we present the results of IL4RA expression analysis in relation to specific polymorphisms within the gene -most of them well-known from association studies, I50V and Q551R, and the less-studied C-3223T.…”

Section: Introduction and Objectivesmentioning

confidence: 99%

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IL4RA gene expression in relation to I50V, Q551R and C-3223T polymorphisms

Danielewicz¹,

Dębińska²,

Drabik-Chamerska³

et al. 2021

Adv Clin Exp Med

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Background. Interleukin 4 (IL-4) and its receptor play important roles in the pathologies of asthma and atopy. The alpha subunit of the IL-4 receptor (IL-4RA) is included in 2 types of receptors which have different modulatory effects on immune responses. This distinct pattern reflects involvement in the immunopathology of both asthma and atopy. A number of studies have proven the association between IL4RA gene polymorphisms and asthma and atopy, but it is still an open question whether these variants are functional. Objectives.To analyze the data from IL4RA gene expression in PBMC in relation to specific polymorphisms -the most frequently studied I50V and Q551R and the less known C-3223T.Material and methods. The analysis was performed for 36 subjects, both atopic and non-atopic. Real-time polymerase chain reaction (PCR) was used with specific primers for the quantification and genotyping. Delta Ct (ΔCT) and delta-delta Ct (ΔΔCT) values were used for the relative quantification of IL4RA expression in PBMC. Results.We observed no significant differences in the IL4RA expression profile between the 3 genotypes. A trend toward higher relative expression was observed for homozygous minor I50V and C-3223T genotypes.Conclusions. We did not find a statistically significant relationship between the genetic polymorphisms and the relative expression of IL4RA. The effect of genetic polymorphism on IL4RA mRNA expression could interfere with other factors, such as environmental stimuli, and should be evaluated in future studies.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introduction and Objectivesmentioning

confidence: 99%

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IL4RA gene expression in relation to I50V, Q551R and C-3223T polymorphisms

Danielewicz¹,

Dębińska²,

Drabik-Chamerska³

et al. 2021

Adv Clin Exp Med

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“…Conversely, plasma levels of the chemoattractant C-C motif chemokine (CCL) 11 have been shown to be lower in urban, compared to rural dwellers in a study investigating the effect of CCL on cognitive function 12 . Among individuals with atopy, urban inhabitants had higher levels of gene expression for IL-4R, a type 2 cytokine receptor, compared with rural inhabitants 13 . In another study in Ghana, urban children had increased gene expression of IL-10 compared with rural children, irrespective of helminth infection status 14 .…”

Section: Introductionmentioning

confidence: 95%

Profiles of inflammatory markers and their association with cardiometabolic parameters in rural and urban Uganda

et al. 2021

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Background: Inflammation may be one of the pathways explaining differences in cardiometabolic risk between urban and rural residents. We investigated associations of inflammatory markers with rural versus urban residence, and with selected cardiometabolic parameters previously observed to differ between rural and urban residents: homeostatic model assessment of insulin resistance (HOMA-IR), fasting blood glucose (FBG), blood pressure (BP) and body mass index (BMI). Methods: From two community surveys conducted in Uganda, 313 healthy individuals aged ≥ 10 years were selected by age- and sex-stratified random sampling (rural Lake Victoria island communities, 212; urban Entebbe municipality, 101). Fluorescence intensities of plasma cytokines and chemokines were measured using a bead-based multiplex immunoassay. We used linear regression to examine associations between the analytes and rural-urban residence and principal component analysis (PCA) to further investigate patterns in the relationships. Correlations between analytes and metabolic parameters were assessed using Pearson’s correlation coefficient. Results: The urban setting had higher mean levels of IL-5 (3.27 vs 3.14, adjusted mean difference [95% confidence interval] 0.12[0.01,0.23] p=0.04), IFN-⍺ (26.80 vs 20.52, 6.30[2.18,10.41] p=0.003), EGF (5.67 vs 5.07, 0.60[0.32,0.98] p<0.00001), VEGF (3.68 vs 3.28, 0.40[0.25,0.56] p<0.00001), CD40 Ligand (4.82 vs 4.51, 0.31[0.12, 0.50] p=0.001) and Serpin-E1 (9.57 vs 9.46, 0.11[0.05,0.17] p<0.00001), but lower levels of GMCSF (2.94 vs 3.05, -0.10[-0.19,-0.02] p=0.02), CCL2 (2.82 vs 3.10, -0.45[-0.70,-0.21] p<0.00001) and CXCL10 (5.48 vs 5.96, -0.49[-0.71,-0.27] p<0.00001), compared to the rural setting. In PCA, the urban setting had lower representation of some classical inflammatory mediators but higher representation of various chemoattractants and vasoactive peptides. HOMA-IR, FBG, BP and BMI were positively correlated with several principal components characterised by pro-inflammatory analytes. Conclusions: In developing countries, immunological profiles differ between rural and urban environments. Differential expression of certain pro-inflammatory mediators may have important health consequences including contributing to increased cardiometabolic risk observed in the urban environment.

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IL-4RA gene expression in PBMC with regard to place of living and atopy status

Abstract: The regulation of IL-4RA gene expression is complex and probably influenced by both genetic and environmental factors, such as farming exposures, which could provide the counterbalance to atopy.

Cited by 2 publications

References 27 publications

IL4RA gene expression in relation to I50V, Q551R and C-3223T polymorphisms

IL4RA gene expression in relation to I50V, Q551R and C-3223T polymorphisms

Profiles of inflammatory markers and their association with cardiometabolic parameters in rural and urban Uganda

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