IL-4 Decreases the Expression of the Monocyte Differentiation Marker CD14, Paralleled by an Increasing Accessory Potency

Ruppert, Jörg; Friedrichs, Detlef; Xu, Hui; Peters, J. H.

doi:10.1016/s0171-2985(11)80209-3

Cited by 81 publications

(37 citation statements)

References 41 publications

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“…Several previous studies showed that DC could develop from CD14 + blood monocytes cultured with GM-CSF plus IL-4 (Mayordomo et al, 1995;Sallusto and Lanzavecchia, 1994), IL-3 plus IL-4 (Ebner et al, 2002), Flt-3L plus IL-4 or by CD40-CD40 ligand interactions (Brossart et al, 1998). IL-4 was shown to downregulate CD14 and CD16 antigens, up-regulate MHC class II molecules and enhance the allo-stimulatory properties of monocytes (Ruppert et al, 1991;Velde et al, 1988). Exposure to aluminium hydroxide led to a significant activation of accessory properties of monocytes and an increase in expression of MHC class-II, CD40, CD54, CD58, CD83 and CD86 molecules (Ulanova et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

IL-4 alone without the involvement of GM-CSF transforms human peripheral blood monocytes to a CD1adim, CD83+ myeloid dendritic cell subset

Roy

Bandyopadhyay

Rakshit

et al. 2004

Journal of Cell Science

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Myeloid dendritic cells (DCs) are conventionally generated by culturing human peripheral blood monocytes in the presence of GM-CSF and IL-4. Here we report that IL-4 alone, in the absence of detectable endogenous GM-CSF, transforms human peripheral blood monocytes to a CD1adim DC subset that could be matured to CD83+ DCs. Absence of endogenous GM-CSF in IL-4-DC was demonstrated by RT-PCR and flow cytometry. With the exception of CD1a expression, surface marker, morphology and phagocytic activity of these DCs (IL-4-DC) were similar to myeloid DCs (GM-IL-4-DC) conventionally generated in the presence of GM-CSF and IL-4. Conventional GM-IL-4-DC produced less IL-12 compared with IL-4-DC after stimulation with anti-CD40 monoclonal antibody, or LPS plus IFN-γ, although the difference was more prominent when LPS plus IFN-γ was used as the stimulus. The GM-IL-4-DC also induced less frequent IFN-γ+ T cells in a mixed leukocyte reaction (MLR) than that of IL-4-DC. Yields of IL-4-DCs were marginally lower than that of GM-IL-4-DCs. Our data indicate that peripheral blood monocytes can be transformed to CD1a-deficient myeloid DCs solely by IL-4, and these IL-4-DCs are likely to induce a stronger Th1 response than conventional GM-IL-4-DCs.

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Section: Discussionmentioning

confidence: 99%

IL-4 alone without the involvement of GM-CSF transforms human peripheral blood monocytes to a CD1adim, CD83+ myeloid dendritic cell subset

Roy

Bandyopadhyay

Rakshit

et al. 2004

Journal of Cell Science

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“…It should be noted that there are disagreements both within the literature and also with our studies regarding the changes brought about in surface antigen expression by CSF-1 and GM-CSF stimulation of human monocytes. For example, CD14 and CD11b expression has been shown to be enhanced in the presence of CSF-1 [1,4]; both markers have been found to be up-regulated, down-regulated, or to show no change in response to GM-CSF [37][38][39][40][41][42][43][44]. Furthermore, HLA-DR has been shown to be up-regulated or to show no change in response to CSF-1 [1,6] and to be up-regulated by GM-CSF [37,45].…”

Section: Discussionmentioning

confidence: 99%

Characterization of a CSF-induced proliferating subpopulation of human peripheral blood monocytes by surface marker expression and cytokine production

Finnin

Hamilton

Moss

1999

Journal of Leukocyte Biology

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The phenotype of a subpopulation(s) of human monocytes which has been shown to proliferate in vitro in response to macrophage colonystimulating factor (M-CSF or CSF-1) and granulocyte-macrophage CSF (GM-CSF) is as yet unknown. To identify this proliferating subpopulation(s) we demonstrated first that DNA synthesis was occurring under culture conditions suitable for flow cytometric evaluation. Flow cytometric analysis of surface antigen expression identified that after 5 days of culture the proliferating subpopulation of monocytes expressed CD14, CD13, CD33, CD11b, CD11c, CD87, HLA-DR, CD45RO, and did not express CD86, CD34, CD80, CD4, CD16, and CD56. In addition, these proliferating monocytes (representing approximately 5% of total monocytes) were shown to produce the proinflammatory cytokines interleukin-6 and tumor necrosis factor ␣ in response to lipopolysaccharide stimulation. Further characterization and subsequent isolation of this subpopulation of monocytes may provide new and important information necessary to understand inflammatory diseases such as rheumatoid arthritis, where local proliferation at the site of inflammation may be a key factor contributing to the chronicity of the disease. J. Leukoc. Biol. 66: 953-960; 1999.

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“…The induction of "monocyte accessory cells" from CD14+ monocytes treated with interleukin 4 has been described (18 (2)(3)(4)(5)(6)(7)(8)(9). In addition, they may lend to the further delineation of the diverse functions of cells with dendritic morphology, as well as a better understanding of the role of such cells in the pathogenesis of HIV disease.…”

Section: Discussionmentioning

confidence: 99%

Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1.

Weissman

Ananworanich

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

163

100

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Conflicting data have been reported with regard to the infectability, dysfunction, and depletion of dendritic cells (DCs) in human immunodeficiency virus (HIV) disease. These discrepancies could potentially be explained by the existence of multiple subsets of cells with dendritic morphology in peripheral blood. The isolation of DCs in humans is accomplished through negative selection until a morphologically pure population is obtained. Recently, DC precursors purified from peripheral blood by negative selection have been observed to develop into functionally and morphologically mature DCs. In this report we identify three populations of cells in peripheral blood that have or can develop a dendritic morphology. The first population, when allowed to mature in culture, develops a dendritic morphology and gains the expression of HB15, a marker of DCs in blood, thymus, skin, and lymphoid organs. The second population expresses HB15 and has the phenotypic and morphologic characteristics of mature DCs. The third population is morphologically very similar to mature DCs but does not share the same T-cell-stimulatory activity and is the only population that is infectable with HIV. Understanding the heterogeneity of cells of dendritic lineage and/or morphology in the peripheral blood will aid in understanding their role as antigenpresenting cells in general and as potential participants in the immunopathogenesis of HIV disease.Dendritic cells (DCs) are bone marrow-derived, antigenpresenting cells that are identified by their exceptional ability to present antigen to and activate T cells; a dendritic morphology; expression of high levels of major histocompatibility complex (MHC) class II and adhesion molecules; and the lack of B, T, natural killer (NK), and monocytic cell surface markers (reviewed in ref.

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IL-4 Decreases the Expression of the Monocyte Differentiation Marker CD14, Paralleled by an Increasing Accessory Potency

Cited by 81 publications

References 41 publications

IL-4 alone without the involvement of GM-CSF transforms human peripheral blood monocytes to a CD1adim, CD83+ myeloid dendritic cell subset

IL-4 alone without the involvement of GM-CSF transforms human peripheral blood monocytes to a CD1adim, CD83+ myeloid dendritic cell subset

Characterization of a CSF-induced proliferating subpopulation of human peripheral blood monocytes by surface marker expression and cytokine production

Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1.

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