IL-33 promotes the migration and proliferation of circulating fibrocytes from patients with allergen-exacerbated asthma

Bianchetti, Lorenza; Marini, Maurizio; Isgrò, Mirko; Bellini, Alberto; Schmidt, Matthias; Mattoli, Sabrina

doi:10.1016/j.bbrc.2012.08.047

Cited by 43 publications

(63 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They circulate as a part of the peripheral blood mononuclear cell (PBMC) subpopulation of total leukocytes (Bucala et al, 1994;Chesney et al, 1998;Abe et al, 2001;Bianchetti et al, 2014) and have light scatter features on flow cytometry analysis similar to those of circulating myeloid dendritic cells (mDCs) and some myeloid progenitor cells (mPCs) (Bianchetti et al, 2014). They constitutively express some of the receptors that mediate the trafficking of other BMderived CD34 + PCs, mDCs, and eosinophils (Els) in asthma (Bellini et al, 2013;Mattoli, 2015), such as the C-X-C motif chemokine receptor 4 (CXCR4) (Abe et al, 2001), the C-C motif chemokine receptors (CCRs) CCR3, CCR5, and CCR7 (Abe et al, 2001;Isgrò et al, 2013a), and the receptor for interleukin (IL)-33 suppressor of tumorigenicity 2 (ST2) (Bianchetti et al, 2012a). Fcs have antigen-presenting function (Chesney et al, 1997;Isgrò et al, 2013b) and proinflammatory activities (Chesney et al, 1998;Bellini et al, 2012;Isgrò et al, 2013a;2013b) relevant to asthma (Mattoli, 2015).…”

Section: Background Information On Fc Identification and Isolationmentioning

confidence: 99%

“…Its major limitation is that it precludes the isolation of a viable population of Fcs for functional analyses, because intracellular staining for COL1 requires prior cell fixation and permeabilization. To address this issue, novel multiple parameter approaches have been developed that allow for the identification and sorting of a pure population of circulating and tissue Fcs by flow cytometry on the basis of the coexpression at high levels on the cell surface of CD45/CD45RO, CD34, CD11b, and CD13 (Bianchetti et al, 2012a;Isgrò et al, 2013a;2013b), which is a distinctive feature of Fcs (Bucala et al, 1994;Chesney et al, 1998;Isgrò et al, 2013b;Bianchetti et al, 2014). Taking advantage of these technical improvements, it has been possible to uncover many functional properties of isolated Fcs and improve our understanding of the molecular mechanisms and signaling pathways involved in their trafficking and activation in asthma (Bellini et al, 2012;Bianchetti et al, 2012a;Isgrò et al, 2013a;2013b), as discussed below.…”

Section: Background Information On Fc Identification and Isolationmentioning

confidence: 99%

“…1). Notably, the expression of CCR3, CCR5, and ST2 is highly upregulated on the surface of circulating Fcs from patients with treatment-resistant severe asthma or suffering from acute disease exacerbations in comparison with the circulating cells from patients with adequately-controlled disease and normal individuals (Isgrò et al, 2013a;Bianchetti et al, 2012a). Moreover, the sputum fluid phase from patients with treatment-resistant severe asthma, but not that from patients with adequately-controlled disease, has a strong chemotactic effect on autologous circulating Fcs, and over 50% of this chemotactic activity is attributable to the combined presence of high amounts of CCL5, CCL11, and CCL24 (Isgrò et al, 2013b).…”

Section: Factors Regulating Fc Trafficking and Activationmentioning

confidence: 99%

“…Since Fcs express CCR7 (Abe et al, 2001), the CCR7 ligands CCL19 and CCL21 (also known as secondary lymphoid cytokine) may potentially contribute to the airway recruitment of these cells, albeit only to a small extent, as suggested by blocking experiments in an animal model of chronic allergic asthma with induced airway remodeling (Mattoli, 2006) and in vitro experiments with Fcs from asthmatic patients (Wang et al, 2015). Epithelial cellderived endothelin-1 (ET-1) contributes to the expansion of the population of recruited Fcs in concert with IL-33 (Schmidt et al, 2003;Bianchetti et al, 2012a;Bellini et al, 2013) (Fig. 1).…”

Section: Factors Regulating Fc Trafficking and Activationmentioning

confidence: 99%

“…In asthmatic patients, the numbers of circulating Fcs are in the normal range values when their disease, irrespective of its severity, is adequately controlled by the treatment they are receiving (Saunders et al, 2009;Bianchetti et al, 2012a; (Table 1). Greatly elevated blood Fc numbers are detectable in patients experiencing a spontaneous asthma exacerbation (Bellini et al, 2012;Isgrò et al, 2013a;Wang et al, 2015) and in patients with treatment-refractory severe disease (Saunders et al, 2009;Bianchetti et al, 2012a; or "chronic obstructive asthma" (Wang et al, 2008;2015), whose disease is scarcely controlled in spite of the intensive treatment with high doses of inhaled corticosteroids or oral corticosteroids in combination with bronchodilators (Table 1).…”

Section: Prognostic Roles In Asthmamentioning

confidence: 99%

See 4 more Smart Citations

Pathogenetic and prognostic roles of bloodborne fibrocytes in asthma

Mattoli

2015

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

Abstract:Bloodborne fibrocytes are cells mobilized from the bone marrow, which express surface antigens commonly ascribed to hematopoietic progenitors and have phenotypic and functional characteristics similar to those of immature mesenchymal cells. They exhibit predominant proinflammatory or profibrotic activities at tissue sites, depending on the host's response to environmental insults and on the characteristics of the cell infiltrate and cytokine milieu. In patients with allergic asthma, fibrocytes egress from the bone marrow and are recruited into the airways after every allergen exposure and during viral infections. Recruited fibrocytes amplify the inflammatory responses driven by T helper type 2 lymphokines and favor viral replication and further inflammation on respiratory virus infections. Persistently elevated blood fibrocyte counts and persisting airway fibrocytosis are present in patients with chronically undertreated or corticosteroid-insensitive asthma, and are linked to an enhanced risk of adverse outcomes because of the major involvement of fibrocytes in the development of structural abnormalities that lead to chronic airflow obstruction in these patients. Consequently, blood fibrocyte count is an emerging biomarker of asthma control and disease progression and its clinical applicability as a new outcome measure deserves further evaluation in large clinical trials.

show abstract

Section: Background Information On Fc Identification and Isolationmentioning

confidence: 99%

Section: Background Information On Fc Identification and Isolationmentioning

confidence: 99%

Section: Factors Regulating Fc Trafficking and Activationmentioning

confidence: 99%

Section: Factors Regulating Fc Trafficking and Activationmentioning

confidence: 99%

Section: Prognostic Roles In Asthmamentioning

confidence: 99%

See 3 more Smart Citations

Pathogenetic and prognostic roles of bloodborne fibrocytes in asthma

Mattoli

2015

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

show abstract

Migratory, metabolic and functional alterations of fibrocytes in type 2 diabetes

Walker¹,

Nissen²,

Geiger³

2018

IUBMB Life

View full text Add to dashboard Cite

Fibrocytes are bloodborne mesenchymal progenitor cells that are recruited to injured tissue sites and contribute to the repair process by acquiring a myofibroblast-like phenotype and producing extracellular matrix components and growth factors. Treatment with normal fibrocytes or their exosomes restores the ability of genetically diabetic mice to heal skin wounds, suggesting the existence of dysfunctional alterations in diabetic fibrocytes. This study compared the migratory, metabolic and functional characteristics of fibrocytes from patients with type 2 diabetes (T2DPs) and healthy controls (HCs). It was found that the frequency of these cells was abnormally low in the peripheral blood of T2DPs. Diabetic fibrocytes showed reduced expression of the C-X-C motif and C-C motif chemokine receptors (CXCR)4, (CCR)5, and CCR7, and demonstrated reduced migration in response to their ligands (CXCL)12, (CCL)5, and CCL21. They exhibited increased expression of the receptor for advanced glycation end product, suppression of the alternative AGE receptor 1, increased intracellular concentrations of AGEs, decreased expression of sirtuin-1 and elevated oxidative stress. In short-term cultures, fibrocytes from T2DPs released larger amounts of proinflammatory cytokines than those from HCs. Unlike normal fibrocytes, diabetic fibrocytes did not exhibit increased expression of type I collagen and α-smooth muscle actin on stimulation with transforming growth factor (TGF)-β1 and this abnormal response was associated with downregulation of TGF-β1 type II receptor on the cell surface. Study findings uncover multiple migratory and functional alterations of diabetic fibrocytes that may contribute to explain why T2DPs experience impaired wound healing and chronic ulcers. © 2018 IUBMB Life, 70(11):1122-1132, 2018.

show abstract

A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma

Schmidt¹,

Mattoli²

2013

Methods in Molecular Biology

View full text Add to dashboard Cite

Airway remodeling is a term used to collectively indicate bronchial structural changes that may lead to irreversible airflow obstruction and progressive decline in lung function in asthmatic patients. Bronchial myofibroblasts contribute to airway remodeling by producing collagenous proteins in the subepithelial zone and by increasing the density of contractile cells in the bronchial wall. A substantial proportion of bronchial myofibroblasts in asthma differentiate from circulating mesenchymal progenitor cells known as fibrocytes. Here, we describe a mouse model of allergic asthma for evaluating the functional role of fibrocytes and myofibroblasts in this disease and the inhibitory effects of novel therapeutic candidates.

show abstract

IL-33 promotes the migration and proliferation of circulating fibrocytes from patients with allergen-exacerbated asthma

Cited by 43 publications

References 36 publications

Pathogenetic and prognostic roles of bloodborne fibrocytes in asthma

Pathogenetic and prognostic roles of bloodborne fibrocytes in asthma

Migratory, metabolic and functional alterations of fibrocytes in type 2 diabetes

A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma

Contact Info

Product

Resources

About