IL-27 and IL-27-producing CD4+ T cells in human tuberculous pleural effusion

Xia, Huan; Ye, Zhijian; Zhou, Qiong; You, Wen-Jie; Ai, Chunqing; Wang, Xiaojuan; Zhai, Kan; Jin, Xiao-Guang; Tong, Zhaohui; Shi, Huan‐Zhong

doi:10.1016/j.tube.2014.07.003

Cited by 17 publications

(15 citation statements)

References 35 publications

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“…CD4 ? T cells were typically peripheral effector memory cells [8]. Our present study extended the previous works and found that IL-27 ?…”

Section: Resultssupporting

confidence: 89%

“…The diagnosis of TPE was established by the presence of MTB in pleural fluid or by demonstration of granulomatous pleurisy on pleural biopsy specimen in the absence of any evidence of other granulomatous diseases. The characteristics of these patients have been described previously [8]. The methods of sample collecting and processing were also stated in our previous study [8].…”

Section: Subjectsmentioning

confidence: 99%

“…The characteristics of these patients have been described previously [8]. The methods of sample collecting and processing were also stated in our previous study [8].…”

Section: Subjectsmentioning

confidence: 99%

“…CD4 ? T cells may represent a terminally differentiated and independent subpopulation of Th cells characterized by unique expression profiles of transcription factors and cytokines [8]. In the present study, we investigated the mechanisms by which IL-27 ?…”

Section: Introductionmentioning

confidence: 99%

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Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy

Zhou

et al. 2015

Lung

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“…CD4 ? T cells were typically peripheral effector memory cells [8]. Our present study extended the previous works and found that IL-27 ?…”

Section: Resultssupporting

confidence: 89%

Section: Subjectsmentioning

confidence: 99%

“…The characteristics of these patients have been described previously [8]. The methods of sample collecting and processing were also stated in our previous study [8].…”

Section: Subjectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy

Zhou

et al. 2015

Lung

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“…An accumulation of lymphocytes, particularly CD4 + T cells, in TPE has been well documented in previous studies, demonstrating that patients who were infected with M. tb may exhibit a strong Th1 immune response to kill M. tb, particularly in the young (26)(27)(28). Therefore, the PD-1/PD-L1 pathway and increased sPD-L1 levels may deliver a potent negative co-inhibitory signal to T cells.…”

Section: A B Cmentioning

confidence: 69%

Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion

Pan

Zhong

Xing

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Soluble and membrane-bound programmed death ligand-1 (sPD-L1 and mPD-L1, respectively) have been demonstrated to participate in the immune suppression of non-small cell lung cancer. However, the contribution of sPD-L1 and mPD-L1 to immune regulation and disease progression in patients with pleural effusions remains unknown. The present study evaluated the levels of sPD-L1 and membrane-bound PD-1/PD-L1 in the peripheral blood and pleural effusions of patients with tuberculous pleural effusion (TPE), malignant pleural effusion (MPE) and non-tuberculous non-malignant pleural effusion (n-TB n-M). Furthermore, selected T lymphocytes and cluster of differentiation (CD)14 + monocytes were co-cultured to investigate the potential effect of the PD-1/PD-L1 pathway in TPE. Levels of sPD-L1 and PD-L1 on CD14 + monocytes were increased in the TPE group, as compared with the MPE and n-TB n-M groups. Furthermore, sPD-L1 levels and the expression levels of PD-L1 on CD14 + monocytes were demonstrated to be positively correlated with interferon (IFN)-γ concentration in pleural effusions. Therefore, IFN-γ may increase the expression of PD-L1 on CD14 + monocytes in vitro. Cell counting kit-8 analysis demonstrated that anti-PD-L1 antibody was able to partially reverse the proliferation of T lymphocytes in the co-culture system. The results of the present study indicated that sPD-L1 or mPD-L1 are associated with the immune regulation and disease progression of TPE, and may serve as possible biomarkers of TPE. Furthermore, sPD-L1 and the PD-1/PD-L1 pathway of TPE may be associated with the Th1 immune response; therefore, an anti-PD-1/PD-L1 pathway suggests a potential immune therapy strategy for the treatment of TPE.

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Impaired T cell function in malignant pleural effusion is caused by TGF‐β derived predominantly from macrophages

Wang

et al. 2016

Intl Journal of Cancer

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Malignant pleural effusion (MPE) is an indication of advanced cancer. Immune dysfunction often occurs in MPE. We aimed to identify the reason for impaired T cell activity in MPE from lung cancer patients and to provide clues toward potential immune therapies for MPE. The surface inhibitory molecules and cytotoxic activity of T cells in MPE and peripheral blood (PB) were analyzed using flow cytometry. Levels of inflammatory cytokines in MPE and PB were tested using ELISA. TGF-β expression in tumor-associated macrophages (TAMs) was also analyzed. The effect of TAMs on T cells was verified in vitro. Lastly, changes in T cells were evaluated following treatment with anti-TGF-β antibody. We found that expression levels of Tim-3, PD-1 and CTLA-4 in T cells from MPE were upregulated compared with those from PB, but levels of IFN-γ and Granzyme B were downregulated (p < 0.05). The amount of TGF-β was significantly higher in MPE than in PB (p < 0.05). TGF-β was mainly produced by TAMs in MPE. When T cells were co-cultured with TAMs, expression levels of Tim-3, PD-1 and CTLA-4 were significantly higher than controls, whereas levels of IFN-γ and Granzyme B were significantly decreased, in a dose-dependent manner (p < 0.05). In vitro treatment with anti-TGF-β antibody restored the impaired T cell cytotoxic activity in MPE. Our results indicate that macrophage-derived TGF-β plays an important role in impaired T cell cytotoxicity. It will therefore be valuable to develop therapeutic strategies against TGF-β pathway for MPE therapy of lung cancer.

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IL-27 and IL-27-producing CD4+ T cells in human tuberculous pleural effusion

Cited by 17 publications

References 35 publications

Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy

Recruitment of IL-27-Producing CD4+ T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy

Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion

Impaired T cell function in malignant pleural effusion is caused by TGF‐β derived predominantly from macrophages

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