IL-23 Gene Expression in PBMCs of Patients with Coronary Artery Disease

Khojasteh-Fard, Maryam; Abolhalaj, Milad; Amiri, Parvin; Zaki, Majid; Taheri, Zahra; Qorbani, Mostafa; Bazzaz, Javad Tavakkoly; Amoli, Mahsa M.

doi:10.1155/2012/629814

Cited by 19 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, decreased IL-23 in PBMCs from patients with coronary artery disease has also been reported. 18 In a mouse model of ischemic stroke, IL-23 levels were upregulated in both the circulation and in the brain. 15 However, the role for IL-23 in atherosclerosis is still unclear.…”

Section: March 2015mentioning

confidence: 99%

Interleukin 23 Levels Are Increased in Carotid Atherosclerosis

Abbas

Gregersen

Holm

et al. 2015

Stroke

View full text Add to dashboard Cite

793S troke is one of the major causes of death and disability worldwide. Approximately 85% of all strokes are ischemic, and 20% to 30% of these are caused by carotid atherosclerosis. Moderate-to high-grade carotid artery stenosis can be detected in 1% to 3% of adults, and the incidence increases with age. [1][2][3] Along with the degree of stenosis, inflammation and plaque composition are important in predicting the risk of clinical symptoms. 4 The atherosclerotic plaque is composed of infiltrating inflammatory cells (eg, monocytes/macrophages, T cells, and dendritic cells [DCs]), smooth muscle cells, and lipids. Plaques prone to rupture have thin fibrous caps and high-grade inflammation. The balance between pro-and anti-inflammatory mediators is therefore of major importance for the fate of the plaque and for the occurrence of adverse events. 5,6Background and Purpose-Interleukin (IL)-23 is a cytokine in the IL-12 family, mainly produced by antigen-presenting cells with a central role in inflammation. We hypothesize that IL-23 is also important in atherogenesis and investigate this in a population with carotid atherosclerosis. Methods-Plasma levels of IL-23 were measured in patients with carotid artery stenosis and in healthy controls. The mRNA levels of IL-23 and its receptor, IL-23R, were measured in atherosclerotic plaques, nonatherosclerotic vessels, peripheral blood mononuclear cells, and plasmacytoid dendritic cells. Results-Our findings were as follows: (1) patients with carotid atherosclerosis (n=177) had significantly raised plasma levels of IL-23 when compared with healthy controls (n=24) with particularly high levels in those with the most recent symptoms. (2) mRNA levels of IL-23 and IL-23R were markedly increased in carotid plaques (n=68) when compared with nonatherosclerotic vessels (n=8-10). Immunostaining showed colocalization to plaque macrophages. (3) Patients with carotid atherosclerosis had increased mRNA levels of both IL-23 and IL-23R in plasmacytoid dendritic cells, but not in peripheral blood mononuclear cells. (4) 14-16 Thus, patients with peripheral arterial disease have raised serum levels of IL-23, and increased IL-23 expression is seen in human carotid lesions.14,16 Zhang et al 17 showed that carriers of a polymorphism in the IL-23R gene, which also gave increased expression of IL-23R in peripheral blood mononuclear cells (PBMCs), had increased risk of coronary artery disease. However, decreased IL-23 in PBMCs from patients with coronary artery disease has also been reported. 18 In a mouse model of ischemic stroke, IL-23 levels were upregulated in both the circulation and in the brain. 15 However, the role for IL-23 in atherosclerosis is still unclear.In this study, we examined the expression of IL-23 in patients with carotid atherosclerosis both systemically and within the atherosclerotic plaque as well as its relation to adverse events during follow-up. We also examined the effect of IL-23 in PBMCs and monocytes from patients with carotid atherosclerosis and healthy controls. Mat...

show abstract

Section: March 2015mentioning

confidence: 99%

Interleukin 23 Levels Are Increased in Carotid Atherosclerosis

Abbas

Gregersen

Holm

et al. 2015

Stroke

View full text Add to dashboard Cite

show abstract

“…Considering all the aforementioned evidences, we hypothesized that some specific inflammatory cytokines might also be associated with risk of restenosis in CAD patients underwent PCI with DES . In addition, based on accumulating evidence, TNF‐α, IL‐1β, IL‐4, IL‐6, IL‐8, IL‐10, IL‐17A, IL‐21, and IL‐23 are the most common inflammatory cytokines in CAD patients, which play important roles in the pathological processes of CAD . Therefore, we enrolled 210 CAD patients underwent PCI with DES to compare the expressions of nine common inflammatory cytokines between restenosis patients and non‐restenosis patients, and we discovered that pre‐operative TNF‐α, IL‐6, IL‐17A, and IL‐23 expressions were increased, while pre‐operative IL‐4 expression was decreased in restenosis patients compared with non‐restenosis patients, indicating that pre‐operative TNF‐α, IL‐6, IL‐17A, and IL‐23 expressions might be associated with increased restenosis risk and pre‐operative IL‐4 expression might be associated with decreased restenosis risk.…”

Section: Discussionmentioning

confidence: 99%

Correlation of pre‐operative circulating inflammatory cytokines with restenosis and rapid angiographic stenotic progression risk in coronary artery disease patients underwent percutaneous coronary intervention with drug‐eluting stents

Sun

Liu

et al. 2019

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background This study aimed to explore the associations of common inflammatory cytokine levels with restenosis and rapid angiographic stenotic progression (RASP) risk in coronary artery disease (CAD) patients underwent percutaneous coronary intervention (PCI) with drug‐eluting stents (DES). Methods Two hundred and ten CAD patients underwent PCI with DES were consecutively recruited, then pre‐operative serum levels of TNF‐α, IL‐1β, IL‐4, IL‐6, IL‐8, IL‐10, IL‐17A, IL‐21, and IL‐23 were determined by ELISA. The 12‐month in‐stent restenosis and RASP of non‐intervened lesion were assessed by quantitative coronary angiography analysis. Results The pre‐operative TNF‐α, IL‐6, IL‐17A, and IL‐23 expressions were increased while IL‐4 expression was decreased in restenosis patients compared with non‐restenosis patients. Further analysis revealed that IL‐6, IL‐8, hypercholesteremia, diabetes mellitus, and HsCRP could independently predict restenosis risk, and subsequent ROC curve revealed that their combination was able to differentiate restenosis patients from non‐restenosis patients with an AUC of 0.951 (95%CI: 0.925‐0.978). Meanwhile, the pre‐operative TNF‐α, IL‐6, IL‐17A, IL‐21, and IL‐23 expressions were increased whereas IL‐4 level was decreased in RASP patients compared with non‐RASP patients. Further analysis revealed that TNF‐α, IL‐6, IL‐23, hypercholesteremia, SUA, HsCRP, and multivessel artery lesions could independently predict RASP risk, and subsequent ROC curve disclosed that their combination could discriminate RASP patients from non‐RASP patients with an AUC of 0.886 (95%CI: 0.841‐0.931). Conclusions This study unveils the potentiality of pre‐operative circulating inflammatory cytokines as markers for predicting restenosis and RASP risk in CAD patients underwent PCI with DES.

show abstract

“…Studies have implied that IL-23 can be involved in the development of CAD (Ausiello et al, 2006;Khojasteh-Fard et al, 2012). For example, IL-23 may promote atherosclerosis through affecting dendritic cells.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have shown that Chlamydia pneumoniae (Cp)-matured dendritic cells may contribute to T-cell mediated immunopathology of atherosclerosis through IL-12 and IL-23 (Ausiello et al, 2006). Another report has presented that IL-23 gene expression is significantly decreased in unstimulated peripheral blood mononuclear cells (PBMCs) of CAD patients compared with those healthy controls (Khojasteh-Fard et al, 2012). As an important part of the IL-23 pathway, IL-23R may also play critical roles in atherosclerosis and atherosclerosis-related diseases.…”

mentioning

confidence: 99%

Functional Polymorphisms in Interleukin-23 Receptor and Susceptibility to Coronary Artery Disease

Zhang

Zhenrong

Zhang

et al. 2014

DNA and Cell Biology

View full text Add to dashboard Cite

As a key element in the T-helper 17 (Th17) cell-mediated inflammatory process, interleukin-23 receptor (IL-23R) may play a crucial role in the pathogenesis of atherosclerosis. Single nucleotide polymorphisms (SNPs) in IL-23R have been studied in several diseases. However, its association with coronary artery disease (CAD) remains unclear. This study examined whether genetic polymorphisms in IL-23R were associated with susceptibility to CAD. Two IL-23R SNPs, rs1884444 and rs6682925, were genotyped in 462 CAD patients and 486 healthy controls. Data showed that percentages of rs6682925TC and CC genotypes were significantly higher in patients than in controls (odds ratio [OR] = 1.23, 95% confidence interval [CI]: 1.04-1.79, p = 0.022; OR = 2.35, 95% CI: 1.52-3.43, p < 0.001, respectively). Frequency of the rs1884444 polymorphism did not reveal any significant differences between patients and healthy donors. Further analyses demonstrated a significantly increased number of rs6682925CC genotype in patients with hypertension. Moreover, we investigated the effect of IL-23R polymorphisms on gene expression by assessing mRNA level of IL-23R in peripheral blood mononucleated cells (PBMCs). Results showed that subjects carrying rs6682925TC and CC genotypes had significantly increased mRNA level of IL-23R in PBMCs than those with TT genotype. These data suggest that IL-23R rs6682925T/C polymorphism may act as a risk factor of CAD.

show abstract

IL-23 Gene Expression in PBMCs of Patients with Coronary Artery Disease

Cited by 19 publications

References 17 publications

Interleukin 23 Levels Are Increased in Carotid Atherosclerosis

Interleukin 23 Levels Are Increased in Carotid Atherosclerosis

Correlation of pre‐operative circulating inflammatory cytokines with restenosis and rapid angiographic stenotic progression risk in coronary artery disease patients underwent percutaneous coronary intervention with drug‐eluting stents

Functional Polymorphisms in Interleukin-23 Receptor and Susceptibility to Coronary Artery Disease

Contact Info

Product

Resources

About