IL-1β increases urinary corin in patients with primary proteinuric kidney diseases and in 293 cells

Abstract

Abstract. Corin is a serine protease that is important for the regulation of blood pressure and water balance. Corin was initially discovered in the heart, however, it has also been detected in kidney cells, though its function in the kidneys is unclear. To further investigate the function of corin in the kidney, the present study analyzed the levels of corin in urine and blood samples collected from normal individuals and patients with primary proteinuric diseases. The associations between the levels of corin, and the cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were then assessed. The results demonstrated that corin was detectable in the urine and plasma following an enzyme-linked immunosorbent assay; the level of corin in the urine was associated with the level of urinary β 2 -microglobulin (P=0.01), which was indicative of renal tubular injury. When compared with normal individuals, the levels of urinary corin in proteinuric patients were markedly increased (P=0.02), and were also associated with IL-1β (P=0.03). This correlation between corin and IL-1β was confirmed in vitro using 293 cells. As the IL-1β concentrations increased (0, 0.1, 1, 10 ng/ml), an elevation in the level of corin was observed in the culture medium (P<0.01); however, the amount of corin was not markedly altered in the cell lysate (P>0.05). In addition, when TNF-α reached 10 ng/ml, the level of corin in the medium increased significantly when compared with the control group (0 ng/ml; P=0.02), however, no significant difference in corin levels was detected in the cell lysate. The results suggest that the cytokines IL-1β and TNF-α may increase urinary corin in patients with primary proteinuric kidney diseases. Cytokines may accelerate corin shedding from the cell membrane of renal tubule epithelial cells. These findings indicate that corin may be associated with kidney inflammation and injury.

Corin is regulated by circ-0012397/miR-200a-3p and inhibits the oxygen-glucose deprivation-induced apoptosis of SHSY5Y neuroblastoma cells

“…Corin has been reported to have anti-inflammatory role in natriuretic peptides treating Congestive heart failure ( 32 ). IL-1β and TNF-α could increase the level of urinary corin in patients with primary proteinuric kidney diseases ( 33 ). Recent studies have reported that corin participates in regulating several physiological signal transduction pathways, such as the MAPK, PI3K/Akt, and NF-κB pathways ( 4 , 5 , 28 ).…”

Corin Is Downregulated in Renal Ischemia/Reperfusion Injury and Is Associated with Delayed Graft Function after Kidney Transplantation

“…Renal Corin and both the mRNA and protein levels were found to be negatively related with chronic kidney disease, supporting that the decrease of renal Corin might be a novel biomarker of kidney injury. Another group reported that IL-1 β increases urinary Corin levels in primary proteinuric kidney diseases [7]. However, none of these studies separately analyzed the roles of renal and cardiac Corin.…”

“…Recent studies have indicated that Corin is also present in renal tissue [5], and the decrease in renal Corin is associated with chronic kidney disease [6]. Another group reported that proinflammation factors such as interleukin-1 β (IL-1 β ) and tumor necrosis factor- α (TNF- α ) upregulate Corin expression in patients with primary proteinuric kidney diseases [7]. However, the role of Corin in renal IR injury remains unclear.…”