IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence

Bryche, Bertrand; Dewaele, Aurélie; Saint-Albin, Audrey; Schlegel, Claire; Congar, Patrice; Meunier, Nicolas

doi:10.1016/j.bbi.2019.02.012

Cited by 23 publications

(32 citation statements)

References 47 publications

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“…The immunohistochemistry analysis of the olfactory mucosa tissue sections was performed as described previously in mice (Bryche et al, 2019a). Briefly, the whole animal head was fixed for 3 days at room temperature in 4% paraformaldehyde PBS, then decalcified for one week (10% EDTA -pH 7.3 at 4°C).…”

Section: Immunohistochemistry Histological Stainings and Quantificatmentioning

confidence: 99%

“…OSNs have cilia in direct contact with the environment in order to detect odorants. They are therefore accessible to respiratory viruses and some of these viruses are able to infect the OSN and reach the central nervous system by following the olfactory nerves which project to the olfactory bulbs (Forrester et al, 2018;Bryche et al, 2019a). Interestingly, SARS-CoV-1 (Netland et al, 2008) was described as a virus able to use this olfactory pathway.…”

Section: Introductionmentioning

confidence: 99%

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Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters

Bryche

Deliot

Murri

et al. 2020

Preprint

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144

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Anosmia is one of the most prevalent symptoms of SARS-CoV-2 infection during the COVID-19 pandemic. However, the cellular mechanism behind the sudden loss of smell has not yet been investigated. The initial step of odour detection takes place in the pseudostratified olfactory epithelium (OE) mainly composed of olfactory sensory neurons surrounded by supporting cells known as sustentacular cells. The olfactory neurons project their axons to the olfactory bulb in the central nervous system offering a potential pathway for pathogens to enter the central nervous system by bypassing the blood brain barrier. In the present study, we explored the impact of SARS-COV-2 infection on the olfactory system in golden Syrian hamsters. We observed massive damage of the OE as early as 2 days post nasal instillation of SARS-CoV-2, resulting in a major loss of cilia necessary for odour detection. These damages were associated with infection of a large proportion of sustentacular cells but not of olfactory neurons, and we did not detect any presence of the virus in the olfactory bulbs.We observed massive infiltration of immune cells in the OE and lamina propria of infected animals, which may contribute to the desquamation of the OE. The OE was partially restored 14 days post infection. Anosmia observed in COVID-19 patient is therefore likely to be linked to a massive and fast desquamation of the OE following sustentacular cells infection with SARS-CoV-2 and subsequent recruitment of immune cells in the OE and lamina propria.

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Section: Immunohistochemistry Histological Stainings and Quantificatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters

Bryche

Deliot

Murri

et al. 2020

Preprint

Self Cite

144

View full text Add to dashboard Cite

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“…Tissue culture and mouse studies showed that epithelial-derived IL-17C mediates the expression of cytokines and chemokines in response to infection with Gramnegative bacteria and viruses, respectively, and the recruitment of neutrophils into the lung through the receptor IL-17RE (6,7,10,12,13,(15)(16)(17). Whether or not IL-17RE/IL-17C signaling plays a role during infection with Gram-positive bacteria is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence that IL-17C promotes the recruitment of neutrophils into the lung through IL-17RE. Tissue culture studies showed that bacteria, viruses, and inflammatory cytokines induce the expression of IL-17C in respiratory and alveolar epithelial cells and that the knockdown of IL-17RE or IL-17C in respiratory epithelial cells decreases the expression of inflammatory cytokines, chemokines, and neutrophil chemotaxis in response to viral and bacterial infection (6,7,10,(12)(13)(14). Moreover, administration of adenoviruses expressing IL-17C and application of recombinant IL-17C result in the recruitment of neutrophils into the lung (15,16).…”

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confidence: 99%

Interleukin 17 Receptor E (IL-17RE) and IL-17C Mediate the Recruitment of Neutrophils during Acute Streptococcus pneumoniae Pneumonia

et al. 2019

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Neutrophils contribute to lung injury in acute pneumococcal pneumonia. The interleukin 17 receptor E (IL-17RE) is the functional receptor for the epithelial-derived cytokine IL-17C, which is known to mediate innate immune functions. The aim of this study was to investigate the contribution of IL-17RE/IL-17C to pulmonary inflammation in a mouse model of acute Streptococcus pneumoniae pneumonia. Numbers of neutrophils and the expression levels of the cytokine granulocyte colony-stimulating factor (G-CSF) and tumor necrosis factor alpha (TNF-α) were decreased in lungs of IL-17RE-deﬁcient (Il-17re−/−) mice infected with S. pneumoniae. Numbers of alveolar macrophages rapidly declined in both wild-type (WT) and Il-17re−/− mice and recovered 72 h after infection. There were no clear differences in the elimination of bacteria and numbers of blood granulocytes between infected WT and Il-17re−/− mice. The fractions of granulocyte-monocyte progenitors (GMPs) were significantly reduced in infected Il-17re−/− mice. Numbers of neutrophils were significantly reduced in lungs of mice deficient for IL-17C 24 h after infection with S. pneumoniae. These data indicate that the IL-17C/IL-17RE axis promotes the recruitment of neutrophils without affecting the recovery of alveolar macrophages in the acute phase of S. pneumoniae lung infection.

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“…To compare the global responses of OSN from the 3 groups of mice, EOG recordings were made from the OE in an opened nasal cavity configuration as described earlier [12]. Mice were euthanized during the light phase 09:00-18:00 to allow continuous recordings during the working day.…”

Section: Electro-olfactogram Recording (Eog) Of the Olfactory Epitheliummentioning

confidence: 99%

First step of odorant detection in the olfactory epithelium and olfactory preferences differ according to the microbiota profile in mice

Naudon

François

Mariadassou

et al. 2020

Behavioural Brain Research

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We have previously provided the first evidence that the microbiota modulates the physiology of the olfactory epithelium using germfree mice. The extent to which changes to the olfactory system depend on the microbiota is still unknown. In the present work, we explored if different microbiota would differentially impact olfaction. We therefore studied the olfactory function of three groups of mice of the same genetic background, whose parents had been conventionalized before mating with microbiota from three different mouse strains. Caecal short chain fatty acids profiles and 16S rRNA gene sequencing ascertained that gut microbiota differed between the three groups. We then used a behavioural test to measure the attractiveness of various odorants and observed that the three groups of mice differed in their attraction towards odorants. Their olfactory epithelium properties, including electrophysiological responses recorded by electro-olfactograms and expression of genes related to the olfactory transduction pathway, also showed several differences. Overall, our data demonstrate that differences in gut microbiota profiles are associated with differences in olfactory preferences and in olfactory epithelium functioning.

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IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence

Cited by 23 publications

References 47 publications

Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters

Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters

Interleukin 17 Receptor E (IL-17RE) and IL-17C Mediate the Recruitment of Neutrophils during Acute Streptococcus pneumoniae Pneumonia

First step of odorant detection in the olfactory epithelium and olfactory preferences differ according to the microbiota profile in mice

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