IL‐13 induced increases in nitrite levels are primarily driven by increases in inducible nitric oxide synthase as compared with effects on arginases in human primary bronchial epithelial cells

Chibana, Kazuyuki; Trudeau, John B.; Mustovitch, A. T.; Hu, Hongsheng; Zhao, Jiang; Balzar, Silvana; Chu, Hang; Wenzel, Sally E.

doi:10.1111/j.1365-2222.2008.02969.x

Cited by 186 publications

(115 citation statements)

References 42 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…IL-4 and IL-13 induce release of the granulocyte survival factor GMCSF [107] and IL-8 [108] from airway epithelium. IL-4 potentiates IFN-c induced [109], and IL-13 independently induces [110], iNOS production from primary bronchial epithelial cells. IL-4 and IL-13 cause eotaxin release from primary human airway smooth muscle cells (HASM); these responses synergize with IL-1b [111] or TNF-a [112].…”

Section: Inflammationmentioning

confidence: 97%

Strategies targeting the IL-4/IL-13 axes in disease

May¹,

Fung²

2015

Cytokine

144

108

View full text Add to dashboard Cite

Section: Inflammationmentioning

confidence: 97%

Strategies targeting the IL-4/IL-13 axes in disease

May¹,

Fung²

2015

Cytokine

144

108

View full text Add to dashboard Cite

“…Air-liquid culture of human primary bronchial epithelial cells was performed using Clonetics s B-ALI™ AirLiquid Interface (ALI) medium. The ALI state was maintained for 7-10 days; previous studies have shown this duration is required for mucociliary differentiation [28,29].…”

Section: Preparation Of Epithelial Cellsmentioning

confidence: 99%

Cigarette smoke augments MUC5AC production via the TLR3-EGFR pathway in airway epithelial cells

Kanai

Koarai

Shishikura

et al. 2015

Respiratory Investigation

View full text Add to dashboard Cite

“…IL-13 promotes NO-synthase activity and NO production, so elevated FeNO is a good index of TH2 inflammation and high levels of IL-13 in bronchial mucosa [50]. In addition, FeNO can also be used as a predictor of steroid responsiveness more consistently than other parameters.…”

Section: Biomarkersmentioning

confidence: 99%

A Critical Evaluation of Anti-IL-13 and Anti-IL-4 Strategies in Severe Asthma

Bagnasco

Ferrando

Varricchi

et al. 2016

Int Arch Allergy Immunol

185

126

View full text Add to dashboard Cite

Asthma is a high-prevalence disease, still accounting for mortality and high direct and indirect costs. It is now recognized that, despite the implementation of guidelines, a large proportion of cases remain not controlled. Certainly, adherence to therapy and the education of patients remain the primary objective, but the increasingly detailed knowledge about the pathogenic mechanisms and new biotechnologies offer the opportunity to better address and treat the disease. Interleukin (IL)-13 and IL-4 appear as the most suitable targets to treat the T helper 2 (TH2)-mediated forms (endotypes) of asthma. IL-13 and IL-4 partly share the same receptor and signaling pathways and both are deeply involved in immunoglobulin E (IgE) synthesis, eosinophil activation, mucus secretion and airways remodeling. Several anti-IL-13 strategies have been proposed (anrukinzumab, lebrikizunab and tralokinumab), with relevant clinical results reported with lebrikizumab. Such studies facilitate better definition of the possible predictive markers of response to a specific treatment (e.g. eosinophils, total IgE, fraction of exhaled nitric oxide and periostin). In parallel, anti-IL-4 strategies have been attempted (pascolizumab, pitakinra and dupilumab). So far, dupilumab was reported capable of reducing the severity of asthma and the rate of exacerbations. IL-13 and IL-4 are crucial in TH2-mediated inflammation in asthma, but it remains clear that only specific endotypes respond to these treatments. Although the use of anti-IL-14 and anti-IL-13 strategies is promising, the search for appropriate predictive biomarkers is urgently needed to better apply biological treatments.

show abstract

IL‐13 induced increases in nitrite levels are primarily driven by increases in inducible nitric oxide synthase as compared with effects on arginases in human primary bronchial epithelial cells

Abstract: These results contrast with many previous results to confirm that Th2 stimuli enhance iNOS expression and activity. While arginase 1 protein decreases in response to IL-13, neither arginase appears to substantially impact nitrite levels in this system.

Cited by 186 publications

References 42 publications

Strategies targeting the IL-4/IL-13 axes in disease

Strategies targeting the IL-4/IL-13 axes in disease

Cigarette smoke augments MUC5AC production via the TLR3-EGFR pathway in airway epithelial cells

A Critical Evaluation of Anti-IL-13 and Anti-IL-4 Strategies in Severe Asthma

Contact Info

Product

Resources

About