IL-10 Measurement in Aqueous Humor for Screening Patients with Suspicion of Primary Intraocular Lymphoma

Abstract

Diagnosis of PIOL is often made months or years after the initial onset of ocular symptoms. Cytology remains the gold standard for diagnosis. However, measurement of IL-10 in the AH is a good screening test to reduce diagnostic delays.

Biopsy techniques and yields in diagnosing primary intraocular lymphoma

“…We [24,26,44,45] and others [27,46] have shown that PIOL can exhibit high IL-10 levels with IL-10:IL-6 ratios greater than 1.0 being suggestive of PIOL [47]. Cytokine levels and IL-10:IL-6 ratios are by no means diagnostic of PIOL, but they can be useful adjunctive tests in corroborating suspicion of PIOL and determining whether there is a durable response to treatment.…”

“…A standard three-port pars plana approach is employed. Because cytology [22] is standard and we frequently use molecular analysis with polymerase chain reaction amplification (PCR) [23][24][25] and cytokine-level analysis [26,27], a complete core vitrectomy is recommended. Undiluted vitreous is first obtained for cytological analysis.…”

The pathologist's perspective on vitreous opacities

“…17,18,20,22,25,35,49,51 Biochemical analysis of the vitreous specimen for interleukin ratios (IL10 : IL6) may also support the diagnosis of retinal lymphoma. [52][53][54] If possible, retinal lymphoma should be distinguished from other types of intraocular lymphoma, namely primary uveal lymphoma and secondary (metastatic) intraocular lymphoma. The reader is referred to detailed reviews outlining the morphological and immunocytological characteristics of these rare entities.…”

Primäre vitreoretinale Lymphome

“…Einige Zentren setzen sich für die Bestimmung der Zytokinlevel innerhalb der okulären Flüssigkeiten ein, um eine weitere diagnostische Hilfestellung zur Zytologie mit Nachweis oder Ausschluss der PVRL zu erhalten [2, 51,55,56,57,58,59,60]. Dies betrifft das Kammerwasser und die Glaskörperflüssigkeit.…”