IL-10 Augments Antibody Production inin VitroImmunized Lymphocytes by Inducing a Th2-Type Response and B Cell Maturation

Propionibacterium AcnesActs as an Adjuvant inin VitroImmunization of Human Peripheral Blood Mononuclear Cells

“…2A), the size and number of which were correlated with the level of antibody production. 14) Although clusters were formed under all conditions, the size and number of clusters greatly increased in PBMCs cultured with P. acnes or CpG-ODN, suggesting that CpG-ODN and P. acnes exhibit a similar ability to activate cell-cell interaction in PBMCs, and possibly antibody production. We determined the antibody production of PBMCs immunized in vitro (Fig.…”

Generation of human monoclonal antibodies against Propionibacterium acnes by applying the phage display method to human peripheral blood mononuclear cells immunized in vitro

“…Human peripheral blood mononuclear cells (PBMCs) were obtained from a healthy donor and were separated by density-gradient centrifugation using a lymphocyte separation medium (LSM; Organon Teknika, Durham, NC, USA), as described previously (Xu et al 2004;Yamashita et al 2002). Experiments throughout this study were carried out in accordance with the principles of the Declaration of Helsinki and the regulations of the ethics committee of Kyushu University's Faculty of Agriculture.…”

Anti-Peptide Antibody Production Elicited byin VitroImmunization of Human Peripheral Blood Mononuclear Cells

“…8) A color development reaction was carried out for 30 min. All experiments were repeated at least 3 times, and reproducibility was confirmed.…”