IL-1 production by human thymic dendritic cells: Studies on the interrelation with DC accessory function

Lafontaine, Marielle; Landry, Diane; Blanc-Brunat, N; Pelletier, Micheline; Montplaisir, S

doi:10.1016/0008-8749(91)90288-m

Cited by 15 publications

(1 citation statement)

References 36 publications

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“…Human thymic DC can produce IL-1 upon LPS stimulation (Lafontaine et al, 1991). However, this production is weak compared to peripheral blood monocytes.…”

Section: Functional Studies Of Dendritic Cellsmentioning

confidence: 99%

Human thymic dendritic cells

Lafontaine

Landry

Montplaisir

1997

Microsc. Res. Tech.

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Human thymic dendritic cells (DC) represent a member of the bone marrow–derived dendritic cell family. They have a dendritic shape and are found in small numbers mainly at the corticomedullary border and in medullary regions of the thymus. Human thymic DC were isolated by density gradient separation, followed by treatment with CD2, CD7, CD1, and CD11b mAb and immunobeads magnetic separation. The resulting population contains 60–75% brightly HLA‐DR+ cells which present the morphological characteristics of DC observed in situ. Extensive phenotypic analysis confirmed that they are of mesenchymal origin and that some express CD11a and CD54 molecules. Freshly isolated DC do not stain with a wide variety of anti‐T‐B and ‐monocyte or ‐macrophage mAb. However, they acquire the CD1 molecule after a few days in culture. By using a cell sorter we obtained 90–95% of purified human thymic DC. Functional studies have shown that human thymic DC are potent activators in mixed lymphocyte reactions, act as accessory cells in mitogenic thymocyte proliferation, increase the thymocyte proliferative response to a toxin signal, and produce IL‐1. They also formed spontaneous physical associations with thymocytes, which raises questions about the implication of DC in differentiation and/or maturation processes of thymocytes. Microsc. Res. Tech. 38:267–275, 1997. © 1997 Wiley‐Liss, Inc.

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“…Human thymic DC can produce IL-1 upon LPS stimulation (Lafontaine et al, 1991). However, this production is weak compared to peripheral blood monocytes.…”

Section: Functional Studies Of Dendritic Cellsmentioning

confidence: 99%