Ikdc

Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases

“…For example, our data show that CD56+ CD7+ cells expressing CD11c can phosphorylate p38 MAPK and express IFN- γ . Several studies have also demonstrated that murine B220+ CD11c+ NK1.1+ populations, originally described as dendritic cells or NK/DCs with both killing and antigen presenting cell capabilities, may actually represent activated NK cells [62–67]. Meticulous assessment of these features may provide a better understanding of the induction of autoimmunity.…”

Interferon-producing killer dendritic cells (IKDC)

“…Similarly to NK cells, IKDC differentiation is dependant on inhibitor of DNA binding (Id) 2 and they express GATA binding protein 3 (GATA3) but not the transcription factor PU.1 and the Ets factor Spi-B (Caminschi et al, 2007;Spits and Lanier, 2007;Welner et al, 2007), both of the latter factors being indispensable for the development of DCs and PDCs, respectively (Nutt et al, 2005;Schotte et al, 2003) IKDCs express an extensive arsenal of NK receptors, inhibitory or activating (reviewed above). Since IKDCs comply with the recently established definition of NK cells as a CD3  NKp46  subset, these cells were ultimately assigned to the NK lineage (Coulombel and Bensussan, 2008;Robbins et al, 2008;Walzer et al, 2007). Finally, genome-wide expression profiling performed on microarray available for a variety of DC subsets or NK cells in different species identified a transcriptional signature that is evolutionarily conserved and specific for each subset (Robbins et al, 2008).…”

Cellules souches pluripotentes de rat :so what ?

“…Deux publications viennent de changer la donne et rapportent la dérivation de cellules souches embryonnaires de rat et montrent la possibilité d'obtenir des rats chiméri-ques avec transmission germinale du patrimoine des ES. C'est l'équipe d'Austin Smith qui a apporté la solution du problème en établissant début 2008 de nouvelles conditions de culture des cellules ES murines, basées sur la combinaison de trois inhibiteurs chimiques 1 appelée « 3i » [4,5] (➜). Alors que les conditions de dérivation « classiques » des ES de souris utilisant un feeder de fibroblastes embryonnaires et le facteur de croissance LIF (leukemia inhibiting factor) n'avaient pas permis l'obtention d'ES de rat, les 3i se sont avérés plus efficaces, entraînant l'expansion en culture de la masse cellulaire interne des Animaux knock-out via la technologie iPS Le développement d'une autre technologie, celle des cellules souches pluripotentes induites (iPS) [8] pourrait également aboutir à la création de rats KO.…”