II-VI Quantum Dots as Fluorescent Probes for Studying Trypanosomatides

Fontes, Adriana; Santos, Beate S.; Chaves, Claudilene R.; Figueiredo, Regina Célia Bressan Queiroz de

doi:10.5772/36175

Cited by 2 publications

(3 citation statements)

References 33 publications

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“…The results showed that there was no significant difference in the parasite growth and viability that could be observed at 24 hours of incubation with QDs (at any tested concentration). However, the number of cells decreases in a dose-dependent manner after 48 hours (Fontes et al 2012).…”

Section: Evaluation Of Biological Toxicity Of Cdte Qds In Tcruzimentioning

confidence: 95%

“…In view of the results obtained from the growth curve, the 72-hour time were standardized for tests that can clarify the toxicity mechanism of CdTe QD in T. cruzi. Research, Society andDevelopment, v. 9, n. 12, e34391211274, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i12.11274 Similarly, Fontes et al (2012) assessed the potential toxicity of CdS / Cd (OH)2 QDs in T. cruzi cells. The results showed that there was no significant difference in the parasite growth and viability that could be observed at 24 hours of incubation with QDs (at any tested concentration).…”

Section: Evaluation Of Biological Toxicity Of Cdte Qds In Tcruzimentioning

confidence: 99%

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Evaluation of Biological Toxicity of CdTe Quantum Dots in Trypanosoma cruzi

Martins

Gomes

Louro

et al. 2020

RSD

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Luminescent semiconductor nanocrystals or quantum dots (QDs) emerge as important fluorescent probes for in vitro and in vivo Trypanosoma cruzi cells studies. However, to ensure applicability to living organisms, several tests still need to be done. Since several toxic events are caused by QDs, such as loss of mitochondrial membrane potential, ROS generation, DNA damage and cell death by autophagy. We performed a review of the literature on mechanisms of cellular uptake, internalization and citotoxicity of nanoparticles including our results about the evaluation of biological toxicity in T. cruzi. We evaluated the possible effects on parasite growth curves in a time - scale of control and incubated cells with different concentrations of CdTe – QDs (0.2; 2.0; 20 and 200µM) to determine the development cells changes. In addition, intracellular ROS were measured by Electron Paramagnetic Resonance Spectroscopy (EPR) technique. According our results, we can infer that the toxic effects of QDs in T. cruzi are dose-dependent and that high levels of ROS are involved in cellular toxicity promoted by higher concentrations of QDs. In summary, parasites labeled with low concentrations of nanoparticles are suitable and can be used as bioimaging tools for living parasites. However, more studies on QDs cytotoxicity need to be carried out.

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Section: Evaluation Of Biological Toxicity Of Cdte Qds In Tcruzimentioning

confidence: 95%

Section: Evaluation Of Biological Toxicity Of Cdte Qds In Tcruzimentioning

confidence: 99%

Evaluation of Biological Toxicity of CdTe Quantum Dots in Trypanosoma cruzi

Martins

Gomes

Louro

et al. 2020

RSD

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“…The QD size obtained by using absorption spectra is in good agreement with TEM and X-Ray diffraction analysis previously reported by us. 30,33 The original concentration was estimated to be 40 mM for these green QDs, using Peng's and collaborators equations. 34…”

Section: Quantum Dot Synthesis and Characterizationmentioning

confidence: 99%

Studies on intracellular delivery of carboxyl-coated CdTe quantum dots mediated by fusogenic liposomes

et al. 2013

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The use of Quantum Dots (QDs) as fluorescent probes for understanding biological functions has emerged as an advantageous alternative over application of conventional fluorescent dyes. Intracellular delivery of QDs is currently a specific field of research. When QDs are tracking a specific target in live cells, they are mostly applied for extracellular membrane labeling. In order to study intracellular molecules and structures it is necessary to deliver free QDs into the cell cytosol. In this work, we adapted the freeze and thaw method to encapsulate water dispersed carboxyl-coated CdTe QDs into liposomes of different compositions, including cationic liposomes with fusogenic properties. We showed that labeled liposomes were able to fuse with live human stem cells and red blood cells in an endocytic-independent way. We followed the interactions of liposomes containing QDs with the cells. The results were minutely discussed and showed that QDs were delivered, but they were not freely diffused in the cytosol of those cells. We believe that this approach has the potential to be applied as a general route for encapsulation and delivery of any membrane-impermeant material into living cells.

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II-VI Quantum Dots as Fluorescent Probes for Studying Trypanosomatides

Cited by 2 publications

References 33 publications

Evaluation of Biological Toxicity of CdTe Quantum Dots in Trypanosoma cruzi

Evaluation of Biological Toxicity of CdTe Quantum Dots in Trypanosoma cruzi

Studies on intracellular delivery of carboxyl-coated CdTe quantum dots mediated by fusogenic liposomes

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