IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets

Alustiza, Fabrisio; Bellingeri, Romina Valeria; Picco, Natalia; Motta, Carlos; Grosso, M.; Barbero, César Alberto; Acevedo, Diego F.; Vivas, Adriana

doi:10.1016/j.vaccine.2016.05.004

Cited by 27 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prophylaxis of bacterial infections in animals for consumption, using IgY, was also considered. Specific IgY incorporated into hydrogel added to carbon nanotubes and chitosan (H-CNT) showed protective activity against enterotoxigenic Escherichia coli (ETEC) in piglets [75]. Thus, Anti-ETEC IgY incorporated into H-CNT and orally applied could be used to prevent the intestinal infection in these animals.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

108

125

View full text Add to dashboard Cite

A B S T R A C TEgg yolk constitutes a relevant alternative source of antibodies. It presents some advantages over mammalian serum immunoglobulins regarding productivity, animal welfare and specificity. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. Moreover, due to structural differences and phylogenetic distance, IgY is more suitable for diagnostic purposes than mammalian antibodies, since it does not react with certain components of the human immune system and displays greater avidity for mammalian conserved proteins. IgY has been extensively used in health researches, as both therapeutic and diagnostic tool. This article aims to review its applications in both human and veterinary health.

show abstract

Section: Antibacterial Activitymentioning

confidence: 99%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

108

125

View full text Add to dashboard Cite

show abstract

“…Furthermore, they used whole bacteria; so all virulence factors were contained within the vaccine. In our research, we used E. coli F4 lysate, a strain with lower pathogenicity for birds because it is a strain owned from pigs (4). …”

Section: Discussionmentioning

confidence: 99%

“…The immunogen was made using an enterotoxigenic E. coli (ETEC) ( E. coli F 4 ) strain with the following virulent factors: F 4 , LT, and STb, originally isolated from piglets with neonatal diarrhea on a farm in Córdoba Province, Argentina (4). …”

Section: Methodsmentioning

confidence: 99%

“…The disabled ETEC’s strain was pelleted by centrifugation (4,000 × g for 5 min), and the pellet was washed five times with sterile phosphate buffer saline (PBS). This was used as the immunogen (bacterin), a concentration of 10 9 UFC/mL mixed with aluminum hydroxide 7% (employed as adjuvant) and stored at 4°C until needed for analyses (4). …”

Section: Methodsmentioning

confidence: 99%

“…This is known as IgY biotechnology (1–3) and has been shown to be effective in the inhibition of several enteric pathogens including Escherichia coli ( E. coli ) in pigs (3–6) and neonatal calves (3). Consequently, antibodies (Ab) developed against, for example, E. coli F 4 could be suitable alternatives for use in newborn piglets, to diminish or eliminate fatal diarrhea infections (4). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4

et al. 2017

Self Cite

View full text Add to dashboard Cite

Immunoglobulin Y (IgY) is the predominant antibody found in hen’s (Gallus domesticus) egg yolk. This antibody, developed against several microorganisms in hen egg yolk, has been successfully used as an alternative to immunoglobulins from mammals for use in immunodiagnostics and immunotherapy. Enteropathogenic Escherichia coli (E.coli) F4 is the main etiological agent associated with swine neonatal diarrhea, and it causes notable economic losses in swine production. The aim of the present study was to evaluate the relationship between humoral immune response and the activation of gut-associated lymphoid tissue (GALT) in laying hens intramuscularly immunized with E. coli F4. Adult laying Shaver hens were immunized with a bacterin based on an inactivated lysate E. coli F4 strain that was originally isolated from neonatal piglet diarrhea, following a recommended schedule. The percentage of B lymphocytes in blood and spleen homogenates was determined by flow cytometry. Villi histomorphometry and the size of germinal centers (GC) activated in GALT and the spleen were measured in histological samples either stained with hematoxylin/eosin or through immunofluorescence. Antibody and isotype-specific antibodies in serum and egg yolk were measured using indirect enzyme-linked immunosorbent assay (ELISA). Secretory and serum immunoglobulin A (IgA) were measured by ELISA tests. Laying hen with intramuscular immunization with E. coli F4 lysate, activated both mucosal and systemic protection. Mucosal protection was provided through B lymphocytes, and most of them were activated on Peyer’s patches and esophageal tonsils, in GALT. Furthermore, increased B lymphocyte number in the lamina propria of the gut, and increased intraepithelial plasmatic cell number, produced high levels of mucosal IgA. Activated B lymphocytes interacted with absorptive cells, immune cells, and microbiota in the gut, producing signals that were translated into a powerful physical defense by producing a greater volume of mucin from an increased number of goblet cells. Systemic protection was provided through B lymphocyte activation of spleen GC, which produced hugely specific IgY serum levels. One week later, this specific IgY was deposited in the yolk. This suggests that GALT is a key immunologic tissue inside the mucosal immune system, acting as the “command center” for humoral reaction.

show abstract

Advances in Carbon Nanotubes–Hydrogel Hybrids in Nanomedicine for Therapeutics

Vashist

Kaushik

Vashist

et al. 2018

Adv Healthcare Materials

151

View full text Add to dashboard Cite

In spite of significant advancement in hydrogel technology, low mechanical strength and lack of electrical conductivity have limited their next-level biomedical applications for skeletal muscles, cardiac and neural cells. Host-guest chemistry based hybrid nanocomposites systems have gained attention as they completely overcome these pitfalls and generate bioscaffolds with tunable electrical and mechanical characteristics. In recent years, carbon nanotube (CNT)-based hybrid hydrogels have emerged as innovative candidates with diverse applications in regenerative medicines, tissue engineering, drug delivery devices, implantable devices, biosensing, and biorobotics. This article is an attempt to recapitulate the advancement in synthesis and characterization of hybrid hydrogels and provide deep insights toward their functioning and success as biomedical devices. The improved comparative performance and biocompatibility of CNT-hydrogels hybrids systems developed for targeted biomedical applications are addressed here. Recent updates toward diverse applications and limitations of CNT hybrid hydrogels is the strength of the review. This will provide a holistic approach toward understanding of CNT-based hydrogels and their applications in nanotheranostics.

show abstract

IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets

Cited by 27 publications

References 32 publications

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Gut-Associated Lymphoid Tissue: A Key Tissue Inside the Mucosal Immune System of Hens Immunized with Escherichia coli F4

Advances in Carbon Nanotubes–Hydrogel Hybrids in Nanomedicine for Therapeutics

Contact Info

Product

Resources

About