IgM-specific serodiagnosis of acute human cytomegalovirus infection using recombinant autologous fusion proteins

Vornhagen, Rolf; Hinderer, Walter; Sonneborn, H.‐H.; Bein, Gregor; Matter, L; The, T. H.; Enders, Gisela; Jahn, Gerhard; Plachter, Bodo

doi:10.1016/0166-0934(96)02047-2

Cited by 14 publications

(24 citation statements)

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“…The p52 recombinant protein is the full ppUL44 protein sequence of HCMV, expressed in Escherichia coli. The CM2 antigen is a combination of two C-terminal sequence portions of ppUL44 and the central sequence of pUL57 (20). IgM antibodies are highly reactive against ppUL44 (p52) and pUL57 (p130) antigens (5,7,8,9,19,20).…”

Section: Discussionmentioning

confidence: 99%

“…The CM2 antigen is a combination of two C-terminal sequence portions of ppUL44 and the central sequence of pUL57 (20). IgM antibodies are highly reactive against ppUL44 (p52) and pUL57 (p130) antigens (5,7,8,9,19,20). p52 and CM2 reactivities are considered early markers of acute HCMV infection (6,8,9,19).…”

Section: Discussionmentioning

confidence: 99%

“…Recombinant proteins, instead of naturally occurring HCMV antigens, are increasingly used to distinguish a primary infection from an established infection (4,5,9,13,18,20). Several attempts have been made to make the best possible antigen combination to ensure a sensitive and specific detection of primary infection (7,8,11,17,19).…”

Section: Discussionmentioning

confidence: 99%

“…Besides classical IgM detection through indirect or capture assays, an alternative could be to detect antibodies against some antigenic targets. It has been shown that reactivity against the recombinant p52 (ppUL44) and CM2 (a recombinant protein of ppUL44 and pUL57) antigens is associated with primary infection (4,5,6,18,20). Reactivity against the p52 and CM2 antigens increases during primary infection.…”

mentioning

confidence: 99%

“…Reactivity against the p52 and CM2 antigens increases during primary infection. A few months after onset, reactivity against p52 sharply falls, while reactivity against CM2 can be detected for several more months in immunocompetent patients (13,20). We evaluated the CMV Multiplex Copalis assay, which is an automated qualitative test that uses coupled light scattering technology to discriminate between recent or past infection.…”

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confidence: 99%

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Reactivity to p52 and CM2 Recombinant Proteins in Primary Human Cytomegalovirus Infection with a Microparticle Agglutination Assay

Nulens

Bodéus

Bonelli³

et al. 2000

Clin Diagn Lab Immunol

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We evaluated the reactivities of sera against p52 and CM2 recombinant antigens of human cytomegalovirus (HCMV), coated on microparticles, for the differentiation of primary HCMV infection from an established infection. Two different test formats of the CMV Multiplex Copalis assay were evaluated. The 214 serum samples tested were immunoglobulin M (IgM) positive or equivocal by our reference assay. Reactivities against p52 and CM2 antigens were tested for sera from 37 patients with a well-documented seroconversion within the preceding 3 months (119 serum specimens), 31 patients known to have had a seroconversion at least 8 months earlier (31 serum specimens), and 57 patients without a documented seroconversion (64 serum specimens). The assay had a sensitivity for the detection of a primary infection of 70 or 86% by the first test format and a sensitivity of 88 or 94% by the second test format, according to the criteria used to indicate a primary infection by this test. A good correlation of the results of the assay with our in-house avidity index was found. The specificity of the assay warrants further evaluation. With IgM-positive sera, the assay was not sufficiently specific to make a distinction between a primary infection and an established infection.Human cytomegalovirus (HCMV) causes morbidity in immunocompromised patients and the fetus in case of pregnancy (2,3,10,12,21). The distinction between a primary infection, reactivation, or convalescence is not easy by serological assays alone, because immunoglobulin M (IgM) antibodies can be present in sera over a long period and can reappear during reactivation (1,13,14). Besides classical IgM detection through indirect or capture assays, an alternative could be to detect antibodies against some antigenic targets. It has been shown that reactivity against the recombinant p52 (ppUL44) and CM2 (a recombinant protein of ppUL44 and pUL57) antigens is associated with primary infection (4,5,6,18,20). Reactivity against the p52 and CM2 antigens increases during primary infection. A few months after onset, reactivity against p52 sharply falls, while reactivity against CM2 can be detected for several more months in immunocompetent patients (13,20). We evaluated the CMV Multiplex Copalis assay, which is an automated qualitative test that uses coupled light scattering technology to discriminate between recent or past infection. It allows the simultaneous detection of antibody reactivity against p52, CM2, and whole-virion protein (VP). MATERIALS AND METHODSHCMV IgM serology. All sera were tested for the presence of HCMV IgM by an indirect enzyme immunoassay (EIA; Enzygnost CMV-IgM; Behring AG, Marburg, Germany). The procedures and interpretation of the results (positive, equivocal, or negative) were as recommended by the manufacturer. This included an absorption of IgG and rheumatoid factor before testing.Patients. In this evaluation 214 serum specimens obtained from 125 patients were tested. All the sera were positive or equivocal for HCMV IgM, as tested by our reference EI...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%