IgM+CD27+ B cells possessed regulatory function and represented the main source of B cell-derived IL-10 in the synovial fluid of osteoarthritis patients

Sun, Huaqiang; Zhang, Yeyong; Song, Weiguo; Yin, Luxu; Wang, Gongteng; Yu, Dejia; Zhang, Qian; Yan, Xinfeng; Li, Shufeng

doi:10.1016/j.humimm.2019.02.007

Cited by 20 publications

(15 citation statements)

References 24 publications

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“…In addition, various proinflammatory factors such as IL-1, IL-1β, IL-6 and TNF-α secreted by the increased number of inflammatory cells, including M1 macrophages, promote the proinflammatory microenvironment of SF further to exacerbate OA. Previous studies have reported that mononuclear cells, including macrophages (7), B (8) and T cells (9), which aggregate during OA synovial tissues and fluid (4,(6)(7)(8), contribute to chondrocyte degradation, local inflammation and activation of innate immune cells. A number of studies have demonstrated that the elevated number of macrophages present in the inflamed synovium and SF serve a major role during OA progression (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

Cui

Wang

2020

Exp Ther Med

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Osteoarthritis (OA) is one of the most prevalent degenerative joint diseases that affects millions of individuals worldwide. During OA, proinflammatory factors (including IL-1, IL-6, IL-17 and TNF-α) are released from chondrocytes and proliferating synoviocytes potentiate the proinflammatory microenvironment of the synovial fluid (SF). The altered SF microenvironment affects the infiltration, polarization and apoptosis of macrophages, though the underlying mechanisms are not completely understood. In the present study, the hypothesis that the knee synovial fluid of patients with knee osteoarthritis (KOA SF) promotes the polarization of peripheral blood mononuclear cell (PBMC)-derived M1 macrophages and inhibits PBMC-derived macrophage apoptosis was investigated. KOA SF increased PBMC-derived macrophage M1 polarization via the microRNA (miR)-155-5p/suppressor of cytokine signaling 1 signaling pathway. Caspase-3 (CASP3) was identified as a novel target of miR-155-5p, where KOA SF inhibited macrophage apoptosis via the miR-155-5p/CASP3 signaling pathway. The results suggested that the proinflammatory environment of KOA SF promoted macrophage M1 polarization and reduced macrophage apoptosis via miR-155-5p. The results provided a potential explanation for the increased number of M1 macrophages observed in KOA SF during OA. In addition, the present study suggested that miR-155-5p may serve as a potential therapeutic target for KOA.

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Section: Introductionmentioning

confidence: 99%

miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

Cui

Wang

2020

Exp Ther Med

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“…In support of this idea are findings in osteoarthritis, in which disease severity is negatively correlated with the number of lesion-infiltrating IL-10 + Bregs (Sun et al, 2019). Thus, future studies are important to establish the potential relationship between the number of Bregs in lesional psoriasis skin and the level of disease activity (PASI).…”

Section: Short-term Deletion Of α4β1-integrin In B Cells Does Not Affmentioning

confidence: 88%

“…However, several studies of extracutaneous inflammation (i.e. CNS inflammation, colitis, and osteoarthritis) also support a localized role for Bregs and link the suppressive function of IL-10 + Bregs to the site of inflammation (Lehmann-Horn et al, 2016, Mishima et al, 2019, Sun et al, 2019.…”

Section: Short-term Deletion Of α4β1-integrin In B Cells Does Not Affmentioning

confidence: 99%

Skin-Homing Regulatory B Cells Required for Suppression of Cutaneous Inflammation

Aira

Debes

2021

Journal of Investigative Dermatology

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“…Although γδ T and CD8 + cells play important roles in protection, CD4 + T cells capable of producing and activating macrophage antimycobacterial functions are thought to be the major immune regulator of MTB [ 15 – 17 ]. Recently, researchers focused their attention on techniques that could more accurately characterize and measure the CD4 + T cell response, such as flow cytometry [ 18 , 19 ]. In this study, we mainly used flow cytometry to analyse the various molecular characteristics of PBMCs (peripheral blood mononuclear cells).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have verified some cytokine secretion profiles from CD4 + T cells in patients with tuberculosis, such as IFN-γ + , TNF-α, IL-2 and CD27 [ 18 , 20 , 21 ]. CD27 is a member of the TNF-receptor superfamily participating in the genesis and development of TB [ 19 , 22 , 23 ]. Nikitina et al suggested that CD27 − IFN-γ + cells play an important role in assessing TB activity, lung destruction, and tissue repair after TB therapy in the majority of smear-positive TB patients [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Zhang

et al. 2021

BMC Immunol

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Background There is a global focus on illness diagnosis in smear-negative and latent tuberculosis infectious populations (SN-TB and LTBI). CD27 has been suggested to play a direct role in active TB. Little is known about smear-negative individuals. Here, we tried to investigate whether it has a role in smear-negative populations. The expression of CD27 and MTB-specific CD27 in CD4+ T cells (“CD27−CD4+” and “CD27−IFN-γ+CD4+”) was evaluated in MTB-unexposed controls (HC), TB contacts (TB-C) and SN-TB individuals by flow cytometry. The sensitivity, specificity and AUC (area under curve) of “CD27−IFN-γ+CD4+” cells to distinguish SN-TBs from HCs and TB-Cs were determined by receiver operating characteristic (ROC) curve analysis. The clinical index was selected from the clinical laboratory and evaluated for correlation with “CD27−IFN-γ+CD4+” cells by Spearman statistical analysis. Results We observed that the percentages of “CD27−IFN-γ+CD4+” cells were significantly increased in the SN-TB group compared with the HC and TB-C groups (AUC was 0.88, sensitivity was 82.14%, specificity was 80.00%, and P < 0.0001). The percentage of “CD27−IFN-γ+CD4+” cells was negatively correlated with WBC (white blood cell count) (r = − 0.3019, P = 0.0182) and positively correlated with IgE (immunoglobulin E) (r = 0.2805, P = 0.0362). Furthermore, “CD27−IFN-γ+CD4+” cells were significantly decreased, especially in the > 50 years group, after clinical treatment. Conclusion The present results demonstrated that the percentage of “CD27−IFN-γ+CD4+” cells might be a conceivable molecular indicator in the diagnosis of SN-TB and was influenced by its outcome of therapy.

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IgM+CD27+ B cells possessed regulatory function and represented the main source of B cell-derived IL-10 in the synovial fluid of osteoarthritis patients

Cited by 20 publications

References 24 publications

miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

miR‑155‑5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis

Skin-Homing Regulatory B Cells Required for Suppression of Cutaneous Inflammation

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

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