IgG subclasses in human periodontal disease

Mackler, Bruce; Waldrop, Thomas C.; Schur, Peter H.; Robertson, Paul B.; Levy, Barnet M.

doi:10.1111/j.1600-0765.1978.tb00159.x

Cited by 80 publications

(1 citation statement)

References 31 publications

(19 reference statements)

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“…In addition, these LPS preparations induced in vitro and in vivo direct PFC responses to SRBC ( Table 5). It has been reported that established lesions of chronic inflammatory periodontal disease in humans are primarily infiltrated with B cell lesions (17,28). Therefore, polyclonal activation of B cells induced by B. gingivalis LPS may participate in the development of periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli

Kinoshita¹,

Nishihara²,

Fujiwara³

et al. 1985

Infect Immun

167

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Lipopolysaccharides (LPSs) were isolated from Bacteroides gingivalis and Escherichia coli by the phenolwater and butanol-water procedures. The phenol-water-extracted LPS from B. gingivalis 381 was composed of 46% carbohydrate, 23% hexosamine, 18% fatty acid, and 5% protein. The major component sugars of this preparation were glucose, glucosamine, rhamnose, galactose, galactosamine, and mannose, and their molecular ratio was 1:0.9:0.7:0.6:0.6:0.4, respectively. Neither heptose nor 2-keto-3-deoxyoctonate was detected. the butanol-water-extracted LPS from this strain was composed of 76% glucose, 7% fatty acid, and 13% protein, and it was associated with a number of polypeptides (13 to 56 kilodaltons). The main fatty acid of both LPS preparations was palmitic acid. It was found that biological activities of LPS from B. gingivalis were comparable to those of LPS from E. coli in terms of activation of the clotting enzyme of Limulus amebocyte lysate, mitogenicity, polyclonal B cell activation, and stimulation of interleukin 1 production in BALB/c mice. Furthermore, LPS-nonresponsive C3H/HeJ spleen cells were found to yield good mitogenic responses to both phenol-water-extracted LPS and butanol-water-extracted LPS from B. gingivalis or butanol-water-extracted LPS from E. coli. On the other hand, spleen cells from LPS-responsive C3H/HeN mice responded well to all these LPS preparations. * Corresponding author. MATERIALS AND METHODS Bacterial strains and growth conditions. B. gingivalis 381 (originally isolated by S. Sockransky, Forsyth Dental Center, Boston, Mass.) and E. coli strain K235 (19) were obtained from Y.

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Section: Discussionmentioning

confidence: 99%

Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli

Kinoshita¹,

Nishihara²,

Fujiwara³

et al. 1985

Infect Immun

167

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Chemotaxis of germinal centers B cells in response to C5a

Kupp¹,

Kosco

Schenkein³

et al. 1991

Eur J Immunol

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An infiltrate of B cells and plasma cells is characteristic of certain chronic inflammatory lesions. However, mechanisms involved in the local accumulation of these cells have not been established. Efforts to demonstrate that B cells from normal animals can migrate in response to inflammation-induced chemoattractants have been inconclusive. The objective of this study was to determine if murine germinal center (GC) B cells could respond chemotactically to a C5a gradient. On successive days after secondary immunization, draining lymph nodes were harvested and the activated GC B cells isolated. These GC B cells were placed in modified Boyden chambers, incubated for 3 h and the distance the leading front of cells migrated through the filters was determined. The results show that GC B cells migrated to factors in zymosan- and lipopolysaccharide-activated serum. The migratory response demonstrated distinct kinetics. Cells isolated between 2 to 4 days after secondary immunization migrated, whereas cells isolated at day 0 and beyond day 6 did not. Checkerboard analysis revealed that the migratory response was attributable to both chemokinesis and chemotaxis. Anti-C5 inhibited the migration of day-3 GC B cells implicating C5 in the migration mechanism. Studies using recombinant C5a established that this C5 fragment was chemotactically active. In conclusion, GC B cells generally were not chemotactically active. However, at a particular stage of maturation B cells in the GC become responsive to C5a as a chemotactic agent. Thus, B cells from normal animals may respond chemotactically, and C5a may play a role in recruitment of recently activated B cells into inflammatory sites.

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Update on B Cell Response in Periodontitis

Demoersman¹,

Pers

2022

Periodontitis

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IgG subclasses in human periodontal disease

Cited by 80 publications

References 31 publications

Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli

Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli

Chemotaxis of germinal centers B cells in response to C5a

Update on B Cell Response in Periodontitis

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