IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients

Daeki, A. O.; Craig, Philip S.; Shambesh, Mohamed

doi:10.1080/00034983.2000.11813546

Cited by 59 publications

(65 citation statements)

References 27 publications

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“…We did not study the specifi c IgG subclasses in relation to cyst types, but their quantifi cation may be important for understanding the natural history of hydatid cyst. IgG 4 antibody response is associated with development, growth, and progression (CE1 to CE3); IgG 1 , IgG 2 , and IgG 3 occur predominantly when cysts became infi ltrated or degenerated (CE4 and CE5) (40).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Cystic Echinococcosis, Central Peruvian Highlands

Gavidia

González

Zhang

et al. 2008

Emerg. Infect. Dis.

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We evaluated prevalence of cystic echinococcosis (CE) in a central Peruvian Highland district by using 4 diagnostic methods: ultrasonography for 949 persons, radiography for 829, and 2 serologic tests for 929 (2 immunoblot formats using bovine hydatid cyst fl uid [IBCF] and recombinant EpC1 glutathione S-transferase [rEpC1-GST] antigens). For the IBCF and rEpC1-GST testing, prevalence of liver and pulmonary CE was 4.7% and 1.1% and seropositivity was 8.9% and 19.7%, respectively. Frequency of seropositive results for IBCF and rEpC1-GST testing was 35.7% and 16.7% (all hepatic cysts), 47.1% and 29.4% (hepatic calcifi cations excluded), and 22.2% and 33.3% (lung cysts), respectively. Weak immune response against lung cysts, calcifi ed cysts, small cysts, and cysts in sites other than lung and liver might explain the poor performance of the serodiagnostic tests. We confi rm that CE is highly endemic to Peru and emphasize the limited performance of available serologic assays in the fi eld.

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Section: Discussionmentioning

confidence: 99%

Diagnosis of Cystic Echinococcosis, Central Peruvian Highlands

Gavidia

González

Zhang

et al. 2008

Emerg. Infect. Dis.

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“…In the chronic phase of CE, elevated antibody levels, particularly IgG, IgM and IgE, occur in humans (Khabiri et al, 2006;Daeki et al, 2000;Pinon et al, 1987;Craig, 1986;Dessaint et al, 1975) (Fig. 2), with IgG1 and IgG4 being predominant (Daeki et al, 2000;Sterla et al, 1999;Wen and Craig, 1994). In murine models of AE infection, IgG and IgM were significantly increased after 9 weeks post-infection (p.i) with eggs (Matsumoto et al, 2010).…”

Section: Antibody Responsesmentioning

confidence: 99%

“…When CE cysts grow, IgG1 and IgG4 levels are elevated, whereas the concentrations of specific IgG1 and IgG4 decline in cases characterized by cyst infiltration or calcification. This indicates that the IgG4 antibody response is also associated with cystic development, growth and disease progression, whereas the IgG1, IgG2 and IgG3 responses occur predominantly when cysts become infiltrated or are destroyed by the host (Daeki et al, 2000).…”

Section: Correlation Of Cytokines With Antibody Productionmentioning

confidence: 99%

“…Anti-oncospheral antibodies play a major role in parasite killing and are central to the protective immune response against E. granulosus (Read et al, 2009). In the chronic phase of CE, elevated antibody levels, particularly IgG, IgM and IgE, occur in humans (Khabiri et al, 2006;Daeki et al, 2000;Pinon et al, 1987;Craig, 1986;Dessaint et al, 1975) (Fig. 2), with IgG1 and IgG4 being predominant (Daeki et al, 2000;Sterla et al, 1999;Wen and Craig, 1994).…”

Section: Antibody Responsesmentioning

confidence: 99%

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Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Zhang¹,

Li²,

Lin³

et al. 2012

Serological Diagnosis of Certain Human, Animal and Plant Diseases

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“…In Egypt, G6 involvement has been shown by a number of studies [13,16,17] . Hydatid cyst fluid (HCF) is antigenically rich, consisting of a complex mixture of glycol-and lipoproteins, carbohydrates and salts, and is therefore considered the main antigenic source for immunodiagnosis of human CE [18] .On the other hand, in the chronic phase of CE, there is frequent occurrence of elevated antibody levels, particularly IgG, IgM, and IgE with predominance of IgG1 and IgG4 subclasses [20] . However, the sensitivity of serological tests is affected by many factors including site, integrity of hydatid cysts and quality of antigen used [21] .…”

Section: Introductionmentioning

confidence: 99%

Antigenic and genotypic characterization of Echinococcus granulosus larval isolates from Egypt

Tonsy

Fikry

Aminou

et al. 2018

Parasitologists United Journal

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Background: Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, is a global zoonotic infection of economic importance constituting a threat to public health in many countries. E. granulosus exists as a complex of different strains that have an impact on the epidemiology and control of CE, the most important of which are G1 and G6 strains. In Egypt, some studies confirm the predominance of G1 strain while others demonstrated the involvement of camel G6 strain in causing human infection. Objective: To study the diagnostic potential of purified antigenic yields of hydatid cyst fluid (HCF) from Egyptian CE patients and DNA corresponding to different recorded genotypes, in addition to the characterization of E. granulosus genotype in human and animal isolates in Egypt. Subjects and Methods: Crude HCF antigens from 30 patients were extracted and fractionated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and probed by enzyme immunoelectrotransfer blot (EITB) against sera from CE patients and 50 controls. HCF from human and animal isolates was obtained and prepared for DNA extraction and the amplification of the predominant genotype bands at 254 bp. Results: PCR applied to HCF protoscolices of human and camels showed a typical 254 bp band of 12S fragment of mitochondrial gene belonging to G6 genotype (camel strain). SDS-PAGE fractionation of crude HCF antigen gave a protein profile composed of 11 bands. Immunoblotting assay showed that anti-E. granulosus IgG of patients' sera recognized 9 antigenic bands, varying in molecular weight from 12-110 kDa. The 48 and 12 kDa bands detected in all patients' sera disappeared after treatment. Conclusion: This study confirmed the predominance of G6 genotype (camel strain) of cystic echinococcosis in Egypt. PCR using the amplification primers of G6 genotype is a promising tool in the diagnosis of CE using either patients' HCF or sera. The use of EITB in the diagnosis and post-treatment follow up of G6 genotype CE patients proved of high sensitivity and specificity. The recognition of 48 and 12 kDa antigenic proteins in 100% of CE cases' sera and their disappearance after treatment marks their usefulness in diagnosis and follow up of CE cases.

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IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients

Cited by 59 publications

References 27 publications

Diagnosis of Cystic Echinococcosis, Central Peruvian Highlands

Diagnosis of Cystic Echinococcosis, Central Peruvian Highlands

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Antigenic and genotypic characterization of Echinococcus granulosus larval isolates from Egypt

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