IgG receptors on foetal chick yolk sac

Linden, Carol D.; Roth, Thomas

doi:10.1242/jcs.33.1.317

Cited by 50 publications

(4 citation statements)

References 24 publications

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“…The protection of young broilers' GI tract against colonization of Campylobacter has been associated with Campylobacter-specific maternal antibodies (MAB) (Sahin et al, 2001;Vandeplas et al, 2010). MAB, predominantly immunoglobulin G (IgG) class, are transported from the egg yolk across the yolk sac membrane into the embryonic circulation to protect young broiler chickens from C. jejuni infections (Linden and Roth, 1978). However, MAB levels change with the increase in the broiler age.…”

Section: Primary Productionmentioning

confidence: 99%

Campylobacter spp. prevalence and mitigation strategies in the broiler production chain

Mota‐Gutierrez

Lis²,

Lasagabaster

et al. 2022

Food Microbiology

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Section: Primary Productionmentioning

confidence: 99%

Campylobacter spp. prevalence and mitigation strategies in the broiler production chain

Mota‐Gutierrez

Lis²,

Lasagabaster

et al. 2022

Food Microbiology

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“…Avian FcRY was first discovered in the yolk sac membrane during embryogenesis ( 4 , 9 ). The uptake of yolk-deposited IgY through the yolk sac membrane dramatically increases in the last few days before hatching ( 1 ), which coordinates enhanced FcRY gene expression in the yolk sac membrane (unpublished data), implying that the yolk-deposited IgY transport to embryonic circulation is a brief event associated with rapid uptake of IgY.…”

Section: Discussionmentioning

confidence: 99%

“…FcRY was isolated from the chicken yolk sac membrane as the receptor responsible for the second step ( 4 , 9 ). FcRY is a member of the mannose receptor family, with 180 kDa as its transmembrane form ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

FcRY is a key molecule controlling maternal blood IgY transfer to yolks during egg development in avian species

Okamoto,

Sasaki,

Ikeda

et al. 2024

Front. Immunol.

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Maternal immunoglobulin transfer plays a key role in conferring passive immunity to neonates. Maternal blood immunoglobulin Y (IgY) in avian species is transported to newly-hatched chicks in two steps: 1) IgY is transported from the maternal circulation to the yolk of maturing oocytes, 2) the IgY deposited in yolk is transported to the circulation of the embryo via the yolk sac membrane. An IgY-Fc receptor, FcRY, is involved in the second step, but the mechanism of the first step is still unclear. We determined whether FcRY was also the basis for maternal blood IgY transfer to the yolk in the first step during egg development. Immunohistochemistry revealed that FcRY was expressed in the capillary endothelial cells in the internal theca layer of the ovarian follicle. Substitution of the amino acid residue in Fc region of IgY substantially changed the transport efficiency of IgY into egg yolks when intravenously-injected into laying quail; the G365A mutant had a high transport efficiency, but the Y363A mutant lacked transport ability. Binding analyses of IgY mutants to FcRY indicated that the mutant with a high transport efficiency (G365A) had a strong binding activity to FcRY; the mutants with a low transport efficiency (G365D, N408A) had a weak binding activity to FcRY. One exception, the Y363A mutant had a remarkably strong binding affinity to FcRY, with a small dissociation rate. The injection of neutralizing FcRY antibodies in laying quail markedly reduced IgY uptake into egg yolks. The neutralization also showed that FcRY was engaged in prolongation of half-life of IgY in the blood; FcRY is therefore a multifunctional receptor that controls avian immunity. The pattern of the transport of the IgY mutants from the maternal blood to the egg yolk was found to be identical to that from the fertilized egg yolk to the newly-hatched chick blood circulation, via the yolk sac membrane. FcRY is therefore a critical IgY receptor that regulates the IgY uptake from the maternal blood circulation into the yolk of avian species, further indicating that the two steps of maternal–newly-hatched IgY transfer are controlled by a single receptor.

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“…Chicken IgG, kindly provided by Dr. John Soper, was purified from egg yolks by a modification of the method of Bernardi & Cook (1960) (Linden & Roth, 1978).…”

Section: Methodsmentioning

confidence: 99%

A specific subunit of vitellogenin that mediates receptor binding

Woods¹,

Roth²

1984

Biochemistry

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Vitellogenin, an estrogen-induced serum protein synthesized in the liver, is composed of two Mr 250K polypeptides. It is specifically transported by a receptor-mediated endocytic process into the developing oocytes of virtually all oviparious animals. Following endocytosis, in the chicken, vitellogenin is specifically processed to yield several smaller products including the phosvitins (PV) and the lipovitellins (LV). These products are then stored within the oocyte until they are degraded during embryogenesis to provide nutrients for the developing embryo. Direct binding studies using iodinated vitellogenin demonstrate that vitellogenin binds to isolated oocyte membranes with a KD of 2.5 microM. Competition studies indicate that PV is a competitive inhibitor of vitellogenin binding. This leads us to propose that the PV portion of the circulating vitellogenin molecule mediates binding and uptake. Direct binding studies using iodinated PV show that PV binds to isolated oocyte membranes with a KD of 2.4 microM. Competition studies also demonstrate that 3.1 microM vitellogenin inhibits 50% of control 125I-PV binding, but IgG and bovine serum albumin at concentrations up to 10 microM have no effect on 125I-PV binding. Another series of competition experiments using a constant amount of vitellogenin and increasing amounts of 125I-PV indicate that vitellogenin acts as a competitive inhibitor of PV binding and has a KI of 2-3 microM. These results support our hypothesis that the receptor which mediates vitellogenin binding and uptake recognizes determinants on the PV portion of the native vitellogenin molecule.

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IgG receptors on foetal chick yolk sac

Cited by 50 publications

References 24 publications

Campylobacter spp. prevalence and mitigation strategies in the broiler production chain

Campylobacter spp. prevalence and mitigation strategies in the broiler production chain

FcRY is a key molecule controlling maternal blood IgY transfer to yolks during egg development in avian species

A specific subunit of vitellogenin that mediates receptor binding

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