IgG antibodies to human herpesvirus-6 in children and adults both in primary Epstein-Barr virus and cytomegalovirus infections

Linde, Annika; Dahl, Helena; Wahrén, Britta; Fridell, Eva; Salahuddin, Z; Biberfeld, Peter

doi:10.1016/0166-0934(88)90058-4

Cited by 75 publications

(34 citation statements)

References 5 publications

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“…[35][36][37] There is no correlation between antibody titers to different herpes viruses and HHV-6 by IFA, ELISA, or immunoblotting, 35,38,39 and the majority of individuals seronegative for CMV and EBV are HHV-6-seropositive. 31 Rising or elevated HHV-6 IgG titers have been described in patients with active CMV or EBV infection. 33,14 This was not a factor in our study group, because nobody had CMV, EBV, or HSV IgM antibodies in the presence of primary HHV-6 infection.…”

Section: Discussionmentioning

confidence: 99%

“…HHV-6 antibody testing was performed by a modification of the method reported by Linde et al 31 Briefly, CPSR-3 (Sigma Chemicals Co, St Louis, MO) adapted HSB-2 cells (ATCC, Rockville, MD) infected with HHV-6 strain U1102 (ATCC, Rockville, MD) were adhered and fixed in water-free acetone on 8-well glass slides. Cell preparations containing 40% infected cells gave a brilliant fluorescence using anti-HHV-6 IgG control serum at a dilution of 1:1280 and an anti-HHV-6 IgM control serum at a dilution of 1:520.…”

Section: Hhv-6 Indirect Immunofluorescent Assay (Ifa)mentioning

confidence: 99%

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Case-Control Study of Primary Human Herpesvirus 6 Infection in Children With Febrile Seizures

Hukin

Farrell²,

MacWilliam

et al. 1998

PEDIATRICS

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ABSTRACT. Rationale. Human herpesvirus 6 (HHV-6) has been demonstrated to be the causative agent in roseola infantum. It has been suggested that HHV-6 may have neurotropic properties and be involved in the pathogenesis of febrile seizures in infants. We describe a case-control study to examine the hypothesis that acute HHV-6 infection occurs more commonly in children with febrile seizures than in controls.Methods. Patients presenting with a first or second febrile seizure between 6 months and 2 years of age were entered in the study. Control patients did not have a seizure but had similar inclusion and exclusion criteria. Specimens were obtained for HHV-6 viral serology and polymerase chain reaction in the acute stage and approximately 2 weeks later. A diagnosis of HHV-6 infection was based on HHV-6-specific IgM and IgG serology and HHV-6 polymerase chain reaction of peripheral blood mononuclear cells and saliva.Results. Eighty-six patients (45 with febrile seizures; 41 controls) were enrolled. The HHV-6 infection status could be determined in only 68 patients (35 with febrile seizures; 33 controls). Acute HHV-6 infection was identified in 15 of 35 febrile seizure patients and in 15 of 33 controls. Evidence of past HHV-6 infection was demonstrated in 13 febrile seizure patients and in 8 controls.Conclusions. The incidence of primary HHV-6 infection is similar in patients with febrile seizures and agematched controls. HHV-6 does not seem to be a major factor in the pathogenesis of first and second febrile seizures. Pediatrics 1998;101(2). URL: http://www. pediatrics.org/cgi/content/full/101/2/e3; human herpesvirus 6, febrile seizures, case-control.ABBREVIATIONS. HHV-6, human herpesvirus 6; RI, roseola infantum; CSF, cerebrospinal fluid; PCR, polymerase chain reaction; PBMN, peripheral blood mononuclear cells; HSV, herpes simplex virus; CMV, cytomegalovirus; EBV, Epstein-Barr virus; ELISA, enzyme-linked immunosorbent assay; IFA, immunofluorescent assay. H uman herpesvirus 6 (HHV-6), a lymphotropic virus, is the causative agent of roseola infantum (RI).1,2 HHV-6 infection is more often associated with a nonspecific febrile illness between 6 months and 2 years of life.3 Seroprevalence studies in Japan, England, and the United States demonstrate that infection with HHV-6 is common.4 -6 HHV-6-specific IgG can be detected in almost all newborns, but the prevalence declines to less than 10% by 4 to 5 months of age, presumably as maternal antibody wanes. 4 The seroprevalence increases to 65% by 1 year of age 6 and to greater than 90% by 13 to 36 months of age. 4,6,7 Accumulating clinical and laboratory evidence suggests that HHV-6, like all other herpesviruses, may have neurotropic properties. RI may be complicated by seizures, encephalopathy, aseptic meningitis, meningoencephalitis, and hemiplegia. Febrile seizures are the commonest cause of seizures in early childhood and present before 3 years of age in more than 80% of patients. 21,22 A family history of seizures, usually febrile seizures, is observed in 30% to 50% o...

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Section: Discussionmentioning

confidence: 99%

Section: Hhv-6 Indirect Immunofluorescent Assay (Ifa)mentioning

confidence: 99%

Case-Control Study of Primary Human Herpesvirus 6 Infection in Children With Febrile Seizures

Hukin

Farrell²,

MacWilliam

et al. 1998

PEDIATRICS

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“…Similar to other serological markers, a subclass-restricted response to antigens has been recently demonstrated (8,9,10,12,18); however, a limited amount of data is available on the virus-specific immunoglobulin G (IgG) subclass responses during the ordinary course of measles viral infection. Narita et al suggested that the IgG3 response could play a major role in acute-phase immunity during primary infection, while the IgG1 response could be related to maintenance of measles immunity (14).…”

mentioning

confidence: 95%

Measles Virus-Specific Immunoglobulin G Isotype Immune Response in Early and Late Infections

et al. 2001

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A total of 154 human serum samples (32 acute-phase and 22 convalescent-phase serum samples obtained within a week and between days 8 and 26 after the onset of rash, respectively, and 100 samples drawn from healthy immune adults) were processed by an immunofluorescence assay for the detection of immunoglobulin M (IgM), total immunoglobulin G (IgG), IgG1, IgG2, IgG3, and IgG4 measles virus-specific antibodies. In the acute phase, IgG1 was seen first, followed by IgG2, IgG3, and IgG4 responses, the mean seropositivity of which gradually increased during convalescence, reaching 100% (standard deviation [SD], 84 to 100%), 57% (SD, 34 to 80%), 86% (SD, 66 to 100%), and 86% (SD, 66 to 100%), respectively. IgG2 rose and fell in connection with Measles has been targeted for global eradication by the World Health Organization's Expanded Programme on Immunization; for the effective control and eventual eradication of measles, it is necessary to impair measles transmission by establishing herd immunity. To accomplish this aim, a sensitive surveillance system is essential to detect wild-virus circulation, as well as sensitive and specific diagnostic tests (4, 5). The diagnosis of measles infection is serologically confirmed by the presence of a fourfold rise in antibody titers for paired acuteand convalescent-phase sera or most often by detection of anti-measles virus immunoglobulin M (IgM) antibody. The performance of IgM detection for the differentiation of primary and secondary measles antibody responses depends upon (i) propagation of the virus within the community, (ii) characteristics of the individual immune response, (iii) time of specimen collection, and (iv) assay sensitivity.Similar to other serological markers, a subclass-restricted response to antigens has been recently demonstrated (8, 9, 10, 12, 18); however, a limited amount of data is available on the virus-specific immunoglobulin G (IgG) subclass responses during the ordinary course of measles viral infection. Narita et al. suggested that the IgG3 response could play a major role in acute-phase immunity during primary infection, while the IgG1 response could be related to maintenance of measles immunity (14).These data offer early support for the hypothesis that the IgG isotypic immune response could also be a useful serologic tool, in addition to specific measles IgM antibody detection, to eventually distinguish between early and late measles infection.The present study was undertaken to point out the specific antiviral IgG1, IgG2, IgG3, and IgG4 subclass response patterns elicited during natural infection (acute and convalescent phases) as well as in the long-lasting humoral immunity to measles virus.The aim of this paper is to contribute to the global understanding of antibody responses to measles virus infections. MATERIALS AND METHODSSerum specimens. A total of 154 positive human serum samples for measles antibodies were used in this study. Serum specimens were classified within two groups according to the characteristics of the measles cases from whic...

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“…However, the mode of transmission of HHV-6 remains actually unclear. Serological studies have revealed that HHV-6 infection is common in healthy adults and children (16,21,24,26,30,31). Most of those HHV-6 infections occur within the first year of life (4,12).…”

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confidence: 99%

Seroprevalence of Human Herpesvirus 6 Infection in Brazilian and Japanese Populations in the North‐East of Brazil

Linhares

Eizuru

Tateno

et al. 1991

Microbiology and Immunology

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The seroprevalence of human herpesvirus 6 (HHV-6) infection was examined for 434 Brazilians and 250 Japanese immigrants living in Recife and its vicinity, in the North-East of Brazil.A total of 684 sera from the healthy individuals were screened for IgG antibodies to HHV-6 by anticomplement immunofluorescence (ACIF) test. The seropositivity rate to HHV-6 showed little difference between the two groups: namely, it was 76.5% for Brazilians and 77.2% for Japanese immigrants. The seropositivity rate was constantly higher in females than in males. The high prevalence of anti-HHV-6antibodies among children indicates that HHV-6 infection occurs very early in life.

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IgG antibodies to human herpesvirus-6 in children and adults both in primary Epstein-Barr virus and cytomegalovirus infections

Cited by 75 publications

References 5 publications

Case-Control Study of Primary Human Herpesvirus 6 Infection in Children With Febrile Seizures

Case-Control Study of Primary Human Herpesvirus 6 Infection in Children With Febrile Seizures

Measles Virus-Specific Immunoglobulin G Isotype Immune Response in Early and Late Infections

Seroprevalence of Human Herpesvirus 6 Infection in Brazilian and Japanese Populations in the North‐East of Brazil

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