IGF2-AS knockdown inhibits glycolysis and accelerates apoptosis of gastric cancer cells through targeting miR-195/CREB1 axis

Yan, Bing; Ren, Zheng; Sun, Jinping; Ding, Chunsheng; Yang, Dongyuan

doi:10.1016/j.biopha.2020.110600

Cited by 18 publications

(11 citation statements)

References 31 publications

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“…Collectively, these results indicated that IGF2-AS may serve as a tumor suppressor in HCC, a potential prognostic biomarker and therapeutic target for HCC patients. Our findings are in line with what have been reported in several other cancers including breast cancer [6], gastric cancer [7], CRC [8], and gastric adenocarcinoma [22].…”

Section: Discussionsupporting

confidence: 93%

“…The miRNAs are small endogenous noncoding RNAs that play essential roles in many biological processes, which are comprehensively dysregulated in cancer cells, contributing to cancer development via many different mechanisms, such as HCC [23,24] Increasing evidences have shown that lncRNAs may serve as ceRNAs of miRNAs to protect the target mRNAs from degradation [25][26][27]. IGF2-AS has been reported to play anti-cancer efficacy via ceRNA mechanism in some other cancers, such as in gastric cancer via miR-195/CREB1 axis [7] and in gastric adenocarcinoma via miR-503/SHOX2 axis [22]. These information implied that IGF2-AS may prevent the carcinogenesis of HCC as ceRNA for specific miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…Increasing studies have verified that long noncoding RNAs (lncRNAs) play key roles during the development of multiple human cancers [ 3–5 ]. Studies have reported that IGF2-AS is a biomarker for cancer diagnosis and prognosis, including as a tumor suppressor in breast cancer [ 6 ], a promotor of glycolysis and apoptosis of gastric cancer cells via targeting miR-195/CREB1 axis [ 7 ], a potential target for colorectal cancer (CRC) treatment [ 8 ], as well as the prognosis and metastasis of gastric adenocarcinoma via serving as a ceRNA of miR-503 to regulate SHOX2 9 . We identified that IGF2-AS was remarkably down-regulated in tumor tissues by analyzing the differentially expressed lncRNAs in HCC based on the RNA-seq dataset in TCGA database (14 tumor and 42 adjacent normal samples) ( Figure 1a ).…”

Section: Introductionmentioning

confidence: 99%

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Over-expression of long non-coding RNA insulin-like growth factor 2-antisense suppressed hepatocellular carcinoma cell proliferation and metastasis by regulating the microRNA-520h/cyclin-dependent kinase inhibitor 1A signaling pathway

Huang

et al. 2021

Bioengineered

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Over-expression of long non-coding RNA insulin-like growth factor 2-antisense suppressed hepatocellular carcinoma cell proliferation and metastasis by regulating the microRNA-520h/cyclin-dependent kinase inhibitor 1A signaling pathway

Huang

et al. 2021

Bioengineered

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“…IGF2, as a mitogen, is the first discovered parentally imprinted gene [ 24 ]. lncRNA IGF2-AS has been proved to play an important role in other diseases, including liver cancer [ 25 ], gastric cancer [ 26 ], and diabetic retinopathy [ 27 ], ect. However, there are still few reports on lncRNA IGF2-AS in sepsis.…”

Section: Discussionmentioning

confidence: 99%

lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients

Liang

Zeng

Gao

et al. 2022

Oxidative Medicine and Cellular Longevity

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Background. Sepsis is one of the major causes of death worldwide, and its high mortality and pathological complexity hinder early accurate diagnosis. We aimed to investigate lncRNA IGF2-AS and HMGA1 effects on pyroptosis of endothelial progenitor cells (EPCs) in sepsis patients and the mechanisms involved. Methods. Blood samples from sepsis patients and healthy subjects were collected, and EPCs were isolated and identified. We constructed cell lines that knocked down lncRNA IGF2-AS, HMGA1, and TYMS. Furthermore, lncRNA IGF2-AS was overexpressed. Subsequently, dNTP treatment with different concentrations was performed to investigate lncRNA IGF2-AS and HMGA1 effects on pyroptosis of EPCs in sepsis patients. Finally, exosomes were isolated from bone marrow mesenchymal stem cells (MSCs) to detect lncRNA IGF2-AS expression, and the influence of MSC-derived exosomal lncRNA IGF2-AS on sepsis was preliminarily discussed. Results. Compared with Healthy group, lncRNA IGF2-AS, HMGA1, and TYMS were highly expressed in Sepsis group. Compared with si-NC group, si-lncRNA IGF2-AS group had increased proliferation ability, decreased pyroptosis, decreased HMGA1, RRM2, TK1, and TYMS expressions. lncRNA IGF2-AS played a regulatory role by binding HMGA1. Compared with si-NC group, the proliferation ability of si-HMGA1 group increased, pyroptosis decreased, and RRM2, TK1, and TYMS expressions also decreased. Compared with si-NC group, pyroptosis in si-TYMS group was reduced. In addition, HMGA1 was related and bound to TYMS. After overexpressing lncRNA IGF2-AS, dNTP level decreased, while the proliferation increased and pyroptosis decreased with higher concentration of dNTP. In addition, we found that EPCs took up MSC-exosomes. Compared with supernatant group, lncRNA IGF2-AS was expressed in exosomes group. Compared with EPCs group, EPCs+exosomes group had increased lncRNA IGF2-AS expression and increased pyroptosis. Conclusions. lncRNA IGF2-AS regulated nucleotide metabolism by mediating HMGA1 to promote pyroptosis of EPCs in sepsis patients. This study provided important clues for finding new therapeutic targets for sepsis.

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“…CircPVT1 has been proved to locate mainly in cytoplasm, which meant that it might act as microRNA sponges to regulate downstream gene expression. MiR-195 is dysregulated in several tumors, such as colon cancer [ 56 ], non-small lung cancer [ 57 ], laryngeal cancer [ 58 , 59 ], gastric cancer [ 60 ], cervical cancer [ 61 ], colorectal cancer [ 62 ], breast cancer [ 63 ], lung adenocarcinoma [ 64 ] and osteosarcoma [ 65 ]. Moreover, miR-195 has been demonstrated that play a significant role in PTC [ 66–69 ].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression

Zeng¹,

Yuan²,

Zhou³

et al. 2021

Bioengineered

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Circular RNAs (circRNAs) have been reported to be involved in the progression of papillary thyroid carcinoma (PTC). However, the role of circular RNA Pvt1 oncogene ( circPVT1) in PTC has rarely been reported. In this study, we aimed to investigate the function and mechanism of circPVT1 in PTC. The expression level of circPVT1, miR-195 and VEGFA were determined by reverse transcription‑quantitative PCR (RT‑qPCR). Fisher’s exact test was used to analyze the correlation between circPVT1 expression and PTC clinical features. Cell Counting Kit-8 (CCK-8) and 5-Ethynyl-2ʹ-deoxyuridine (EdU) staining assay and transwell assay were conducted to evaluate the cell proliferation, migration and invasion ability. Dual-luciferase reporter and Western blot assay were conducted for evaluating the correlation between miR-195 and circPVT1 or VEGFA . The results of RT-PCR showed that the expression level of circPVT1 was significantly upregulated in PTC tissues and cell lines. After downregulating circPVT1 expression in PTC cells, the abilities of cell proliferation, migration, and invasion were obviously suppressed, and the Wnt/β-catenin signaling pathway was also repressed. Besides, miR-195 could both bind to PVT1 and VEGFA , while PVT1 could promote the expression of VEGFA by binding to miR-195 . Downregulation of VEGFA expression in PTC cells revealed weakened cell proliferation, migration, and invasion capacities, and restrained Wnt/β-catenin signaling pathway. Therefore, we demonstrated that circPVT1 could promote VEGFA expression by sponging miR-195. CircPVT1 could serve as a molecule sponge for miR-195 and mediate the ceRNA network to promote the expression of VEGFA , thus contributed to the malignant progression of PTC.

show abstract

IGF2-AS knockdown inhibits glycolysis and accelerates apoptosis of gastric cancer cells through targeting miR-195/CREB1 axis

Cited by 18 publications

References 31 publications

Over-expression of long non-coding RNA insulin-like growth factor 2-antisense suppressed hepatocellular carcinoma cell proliferation and metastasis by regulating the microRNA-520h/cyclin-dependent kinase inhibitor 1A signaling pathway

Over-expression of long non-coding RNA insulin-like growth factor 2-antisense suppressed hepatocellular carcinoma cell proliferation and metastasis by regulating the microRNA-520h/cyclin-dependent kinase inhibitor 1A signaling pathway

lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients

Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression

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