IGF1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa

Selvaraju, Sellappan; Krishnan, Binsila B.; Archana, Santhanahalli Siddalingappa; Ravindra, J.P.

doi:10.1016/j.cryobiol.2016.05.012

Cited by 34 publications

(21 citation statements)

References 32 publications

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“…Addition of IGF-I before freezing to ram semen also increased PLM (PADILHA et al, 2012) while the addition of IGF-I to cooled ram (0 to 5°C for 48 h; MAKAREVICH et al, 2014) and boar (15°C for 72 h; MENDEZ et al, 2013) semen increased TM. IGF-I improves spermatozoa motility by reducing oxidative stress (SELVARAJU et al, 2016). Thus, the addition of IGF-I in combination with a PUFA is important to avoid increased lipid peroxidation, although Kiernan et al (2013) found detrimental effects on PLM after the addition of 10 to 100 µM of IGF-I to liquid stored bull semen.…”

Section: Discussionmentioning

confidence: 99%

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Silva

Holden²,

Souza³

et al. 2019

R. Agrogeoambiental

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High seminal quality is important to achieve acceptable pregnancy rate following artificial insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C and ressuspended to 100 x 10 6 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C, packed into 0.5 mL straws, frozen and stored under liquid nitrogen at-196 °C. Straws were thawed at 37 °C for 30 seconds, semen was diluted to 25 x 10 6 spermatozoa/mL and split in four treatments adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1 + IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).

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Section: Discussionmentioning

confidence: 99%

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Silva

Holden²,

Souza³

et al. 2019

R. Agrogeoambiental

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“…An ejaculate having less percentage of total motile spermatozoa may fail to reach the site of fertilization due to lack of motility regulating factors secreted in epididymis (Fu-Jun & Xiao-Fang, 2012;Yoon et al, 2016) and proteins such as CatSper, IGF1, and ODF1 (Tamburrino et al, 2014;Cabrillana et al, 2017;Selvaraju et al, 2016b) BSP1 (Somashekar et al, 2015), and TIP39 (Selvaraju et al, 2016a). An ejaculate having less percentage of total motile spermatozoa may fail to reach the site of fertilization due to lack of motility regulating factors secreted in epididymis (Fu-Jun & Xiao-Fang, 2012;Yoon et al, 2016) and proteins such as CatSper, IGF1, and ODF1 (Tamburrino et al, 2014;Cabrillana et al, 2017;Selvaraju et al, 2016b) BSP1 (Somashekar et al, 2015), and TIP39 (Selvaraju et al, 2016a).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, total sperm motility, percentage of type-A sperm, sperm acrosome reaction, sperm fructose uptake, and sperm intracellular calcium signaling were significantly higher in fertile group than the low fertile and infertile groups. An ejaculate having less percentage of total motile spermatozoa may fail to reach the site of fertilization due to lack of motility regulating factors secreted in epididymis (Fu-Jun & Xiao-Fang, 2012;Yoon et al, 2016) and proteins such as CatSper, IGF1, and ODF1 (Tamburrino et al, 2014;Cabrillana et al, 2017;Selvaraju et al, 2016b) BSP1 (Somashekar et al, 2015), and TIP39 (Selvaraju et al, 2016a). The low percentages of acrosomal reacted spermatozoa of low and infertile groups might suggest the inability of spermatozoa to undergo acrosome exocytosis and lack of calcium-mediated signaling cascades further affects penetration of zona pellucida (Tranter et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Comparative sperm protein profiling in bulls differing in fertility and identification of phosphatidylethanolamine‐binding protein 4, a potential fertility marker

et al. 2017

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This study aimed to identify sperm proteomic signatures regulating sperm functions and fertility by: (i) comparing the sperm electrophoretic protein profiles and identifying the differentially abundant proteins among breeding bulls differing in fertility status and (ii) elucidating the possible role of one of the identified novel proteins, PEBP4 on sperm function and fertility. The grouping of bulls as fertile (n = 6) and low fertile (n = 6) was performed based on bull fertility index and infertile (n = 6) based on semen rejection rate (>33%). The sperm motility, fructolysis index, acrosomal reaction, intracellular calcium levels, and seminal plasma fructose and calcium levels were studied among fertility groups. The differentially expressed sperm proteins observed in single- and two-dimensional gel electrophoresis (2DE) were identified using Nano-LC-MS/MS. In the fertile bulls, the expression levels of calmodulin (CALM1), spermadhesinZ13 (SPADH2), and phosphatidylethanolamine-binding protein 4 (PEBP4) were significantly (p < 0.05) higher than in other fertility groups. In bovine, expression of PEBP4 a novel seminal protein was not observed in spermatozoa of infertile bulls. When the bulls were grouped based on the presence (n = 8) or absence (n = 10) of PEBP4 protein in spermatozoa, a positive significant (p < 0.05) association of this protein with the percentage of motile, type-A spermatozoa, and sperm fructose uptake was observed. Further, PEBP4 was localized in elongated spermatids, Leydig cells, excurrent duct system, and principal piece of spermatozoa. These findings suggest a crucial role for the PEBP4 protein in spermiogenesis, epididymal sperm maturation, and sperm motility. This first study in bovine indicates the positive association of PEBP4 in regulating sperm maturation, functions, and fertility and could be a potential marker for predicting semen quality and fertility.

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“…The significant improvement in sperm parameters may be attributed to the numerous secreted bioactive factors mainly growth factors VEGF, granulocyte-colony stimulating factor (G-CSF), IGF-1, HGF, NGF, and FGF (13), anti- inflammatory cytokines IL-10 and transforming Growth Factor- β (TGF- β ) (13), and antioxidant defense constituents, more particularly superoxide dismutase (SOD) and catalase (20). Actually it was reported that VEGF, HGF, FGF2, NGF and IGF-1 were capable of maintaining and improving sperm motility by stabilizing sperm membrane proteins (28–31). In addition, NGF was proven to decrease sperm DNA fragmentation in both normal spermatozoa (32) and asthenozoospermic samples (33), whereas G-CSF was found to have a protective effect on sperm by decreasing DNA damage and oxidative stress (34).…”

Section: Discussionmentioning

confidence: 99%

Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect

Bader

Ibrahim

Mourad

et al. 2019

IJSC

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Background and ObjectivesOxidative stress (OS) is known to be an important factor of male infertility. Adipose-derived mesenchymal stem cells (AD-MSCs) are known to have immune-modulatory and anti-oxidant effects through their secretions, hence raising the idea of their potential benefit to improve sperm parameters. This study aims at investigating the effect of AD-MSCs conditioned medium (CM) on human sperm parameters in the presence and absence of H2O2-induced OS.Methods and ResultsSperm samples were collected from 30 healthy men and divided into two groups: non-stressed and H2O2-stressed. Isolated AD-MSCs from healthy donors undergoing liposuction were cultured and CM was collected at 24, 48 and 72 h. Both sperm groups were cultured with CM and a time course was performed followed by an evaluation of sperm parameters. The incubation of non-stressed and stressed sperm samples with AD-MSCs-CM for 24 h was found to have the optimum impact on sperm vacuolization, DNA fragmentation and OS levels in comparison to other incubation timings, while preserving motility, viability and morphology of cells. Incubation with CM improved all sperm parameters except morphology in comparison to the non-treated group, with the best effect noted with CM collected at 24 h rather than 48 or 72 h for sperm vacuolization and DNA fragmentation. When compared to fresh semen parameters (T0), samples cultured with CM 24 h showed a significant decrease in sperm vacuolization and DNA fragmentation while keeping other parameters stable.ConclusionsAD-MSCSs-CM improves sperm quality, and hence can be used in treating infertility and subsequently enhancing IVF outcomes.

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IGF1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa

Cited by 34 publications

References 32 publications

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Comparative sperm protein profiling in bulls differing in fertility and identification of phosphatidylethanolamine‐binding protein 4, a potential fertility marker

Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect

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