IGF‐I, IGF‐II, and IGF‐receptor‐1 transcript and IGF‐II protein expression in myostatin knockout mice tissues

Kocamiş, Hakan; Gahr, Scott A.; Batelli, Lori; Hubbs, Ann F.; Killefer, J.

doi:10.1002/mus.10160

Cited by 29 publications

(23 citation statements)

References 26 publications

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“…It is worth noting that IGF-II immunoreactivity in skeletal and heart muscle, shown in this study, matches our results with respect to the cellular localization of myostatin in fish (Radaelli et al 2003b), which is known as a negative regulator of muscle growth in mammals. The reciprocal changes in the expression of myostatin and IGF-II and IGF-IR have been suggested in myostatin-knockout mice (Kocamis et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Expression and cellular localization of insulin-like growth factor-II protein and mRNA in Sparus aurata during development

Radaelli¹,

Patruno²,

Maccatrozzo³

et al. 2003

Journal of Endocrinology

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The spatial localization of IGF-II protein and mRNA was investigated during larval and postlarval developmental stages of the gilthead sea bream (Sparus aurata) by immunohistochemistry and in situ hybridization, using specific antisera and riboprobes. Steady-state levels of IGF-II mRNA in larvae were determined by Northern blot analysis and were found to be increased. Immunoreactivity towards IGF-II was found in larval skin, muscle, gills, gut, olfactory epithelium and kidney. After metamorphosis, the strongest immunoreactivity was found in red skeletal muscle. Positive reaction with IGF-II antibodies was also found in the olfactory epithelium and in the epithelia of pharynx, oesophagus, stomach and kidney. In the adult, the most intense signal was observed in the red and pink musculature and in heart musculature. Immunostaining was also found in saccus vasculosus, thymus, spleen and ovary. IGF-II mRNA was detected by in situ hybridization in the brain, olfactory epithelium, eye, pharynx, skeletal musculature and liver. The spatial distribution of IGF-II shown in this study is consistent with previous findings on the cellular localization of IGF type 1 receptor in the sea bream and supports a role for IGF-II during development and growth of sea bream. Furthermore, these results suggest that IGF-II acts in an autocrine/paracrine manner.

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Section: Discussionmentioning

confidence: 99%

Expression and cellular localization of insulin-like growth factor-II protein and mRNA in Sparus aurata during development

Radaelli¹,

Patruno²,

Maccatrozzo³

et al. 2003

Journal of Endocrinology

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“…IGF-1 gene expression is similar between control and myostatin knockout mice (Kocamis et al, 2002). Recent evidence indicates that myostatin expression in C2C12 cells is induced by IGF-1 (Yang et al, 2007).…”

Section: Introductionmentioning

confidence: 95%

Myostatin and MyoD family expression in skeletal muscle of IGF‐1 knockout mice

Miyake

Hayashi

Satō

et al. 2007

Cell Biology International

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Insulin-like growth factor-1 (IGF-1) is a positive regulator in proliferation and differentiation of skeletal muscle cells, while myostatin (MSTN) is a member of transforming growth factor beta superfamily that acts as a negative regulator of skeletal muscle mass. The present study was performed to detail whether a correlation exists between MSTN and IGF-1 in skeletal muscle of IGF-1 knockout mice (IGF-1(-/-)) and their wild type (WT; i.e., IGF-1(+/+)) littermates. The body weight of IGF-1(-/-) animals was 32% that of WT littermates. The fiber cross-sectional areas (CSA) and number of fibers in M. rectus femoris of IGF-1(-/-) animals were 49 and 59% those of WT animals, respectively. Thus, muscle hypoplasia of IGF-1(-/-) undoubtedly was confirmed. Myostatin mRNA levels and protein levels were similar between M. gastrocnemius of IGF-1(-/-) and WT animals. Myostatin immunoreactivity was similarly localized in muscle fibers of both IGF-1(-/-) and WT M. rectus femoris. The mRNA levels of MyoD family (Myf5, MyoD, MRF4, myogenin) were differentially expressed in IGF-1(-/-)M. gastrocnemius, in which the mRNA expression of MRF4 and myogenin was significantly lower, whereas there were no changes in the mRNA expression of Myf5 and MyoD. These findings first describe that myostatin expression is not influenced by intrinsic failure of IGF-1, although MRF4 and myogenin are downregulated.

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“…In vitro, exogenous Mstn decreased Igf2 but not Igf1 in primary myoblasts [16]. Myostatin null (MN) mice have been previously used to determine how loss of Mstn affected IGF family member expression [16,17].…”

Section: Introductionmentioning

confidence: 99%

Elevated insulin-like growth factor 2 expression may contribute to the hypermuscular phenotype of myostatin null mice

Clark

Hogan

et al. 2015

Growth Hormone & IGF Research

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IGF‐I, IGF‐II, and IGF‐receptor‐1 transcript and IGF‐II protein expression in myostatin knockout mice tissues

Cited by 29 publications

References 26 publications

Expression and cellular localization of insulin-like growth factor-II protein and mRNA in Sparus aurata during development

Expression and cellular localization of insulin-like growth factor-II protein and mRNA in Sparus aurata during development

Myostatin and MyoD family expression in skeletal muscle of IGF‐1 knockout mice

Elevated insulin-like growth factor 2 expression may contribute to the hypermuscular phenotype of myostatin null mice

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