IGF-I enhances survival of embryonic chick ciliary ganglion neurons in a calcium-dependent way

Barale, E.; Torre, Marina; Haimann, C.; Lovisolo, Davide

doi:10.1097/00001756-199808030-00016

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Two hours after plating, neurons were clearly distinguishable from nonneuronal cells by their phase‐bright, rounded cell bodies and short processes. We have previously shown that all birefringent, round cells display electrophysiological properties of differentiated neurons (Distasi et al, 1998) and are positive for neuronal markers (Barale et al, 1998). Each experiment was made in duplicate; three or four independent experiments were performed for each individual treatment.…”

Section: Methodsmentioning

confidence: 97%

Specificity of the second messenger pathways involved in basic fibroblast growth factor‐induced survival and neurite growth in chick ciliary ganglion neurons

Gilardino

Farcito

Zamburlin

et al. 2009

J of Neuroscience Research

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Basic fibroblast growth factor (bFGF) exerts multiple neurotrophic actions on cultured neurons from the ciliary ganglion of chick embryo, among them promotion of neuronal survival and of neurite outgrowth. To understand the specificity of the signal transduction cascades involved in the control of these processes, we used pharmacological inhibitors of the three main effectors known to act downstream of the bFGF receptor (FGFR): phospholipase Cgamma (PLCgamma), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3-K). Neuronal survival was assessed at 24 and 48 hr; neurite growth was analyzed both on dissociated neurons and on explants of whole ganglia. Our data show that only the PI3-K pathway is involved in the survival-promoting effect of bFGF; on the other hand, all three effectors converge on the enhancement of neurite outgrowth, both on isolated neurons and in whole ganglia.

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Section: Methodsmentioning

confidence: 97%

Specificity of the second messenger pathways involved in basic fibroblast growth factor‐induced survival and neurite growth in chick ciliary ganglion neurons

Gilardino

Farcito

Zamburlin

et al. 2009

J of Neuroscience Research

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“…In several mice model of ASD, IGF-II has been suggested as a potential novel therapeutic target for ASD [10]. IGFs have been shown to play important role in the survival of neurons [11]. Many studies in both animal and human ASD models have proven IGF1 as one of the most promising ASD therapeutic interventions to date.…”

Section: Introductionmentioning

confidence: 99%

Comparison of serum IGF1, IGF2 and IGFBP1-6 concentration in the children with different stages of autism spectrum disorders

Mashayekhi¹,

Shabani²,

Salehi³

2022

Arch Psych Psych

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Aim of the studyAutism spectrum disorder (ASD) is a neurodevelopmental condition considered by early-onset difficulties in social communication. Insulin-like growth factors (IGFs) play crucial roles in synapse formation. Most of circulating IGFs are bound to IGF-binding proteins (IGFBPs) that modify IGF action. IGFBPs prolong the plasma half-life of IGFs. In this project we studied the association of IGF1/2 and IGFBP1-6 serum concentration with the severity of ASD (Levels 1-3; Mild, Moderate and severe, respectively).Subject or material and methodsA hundred and eighty patients with ASD (Mild; n=69, Moderate; n=58 and Severe; n=53) and 118 controls age matched were used in this project and IGF1/2 and IGFBP1-6 serum concentration were measured by ELISA.ResultsThe results demonstrated that IGF1, IGF2 and IGFBP1-6 were present in all serum samples. The results showed that the concentration of IGF1 and IGF2 was significantly higher in ASD patients when compared to controls, starting from stages I to III ASD, a significant increase of IGF1 and IGF2 serum concentration was observed. Results obtained also showed that IGFBP1-6 concentration in the ASD group were lower when compared to controls and low serum IGFBP1-6 concentration is associated with advanced stages of ASD.DiscussionIGFs plays important role in synapse formation and changes in IGFs expression may be important in the pathogenesis of ASD.ConclusionsIt is suggested that IGFs and IGFBPs may be involved in the pathogenesis of ASD. Therefore, the detection of serum soluble IGFs and IGFBPs may be useful in classifying ASD.

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Potentiation of Neuronal L Calcium Channels by IGF-1 Requires Phosphorylation of the α1 Subunit on a Specific Tyrosine Residue

Bence

Marshall

Blair

2000

Neuron

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Insulin-like growth factor 1 (IGF-1) rapidly potentiates N and L calcium channel currents in cerebellar granule neurons by an unknown mechanism. Here, we show that the L channel alpha1C subunit is tyrosine phosphorylated in response to IGF-1. Moreover, expression of kinase-dead c-Src in neurons or acute block of Src family kinases with a cell-permeable inhibitor specifically blocks L channel potentiation. Purified Src kinase phosphorylates tyrosine residue Y2122 of the C terminus of neuronal alpha1C in vitro, and c- and v-Src directly bind the C terminus. When expressed in neuroblastoma cells, point mutation of Y2122 prevents both tyrosine phosphorylation of alpha1C and IGF-1 potentiation. Our data provide a biochemical mechanism whereby phosphorylation of a single specific tyrosine residue rapidly modifies ion channel physiology.

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IGF-I enhances survival of embryonic chick ciliary ganglion neurons in a calcium-dependent way

Cited by 3 publications

References 14 publications

Specificity of the second messenger pathways involved in basic fibroblast growth factor‐induced survival and neurite growth in chick ciliary ganglion neurons

Specificity of the second messenger pathways involved in basic fibroblast growth factor‐induced survival and neurite growth in chick ciliary ganglion neurons

Comparison of serum IGF1, IGF2 and IGFBP1-6 concentration in the children with different stages of autism spectrum disorders

Potentiation of Neuronal L Calcium Channels by IGF-1 Requires Phosphorylation of the α1 Subunit on a Specific Tyrosine Residue

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