IGF-1 induces human myotube hypertrophy by increasing cell recruitment

Jacquemin, Virginie; Furling, Denis; Bigot, Anne; Butler-Browne, Gillian; Mouly, Vincent

doi:10.1016/j.yexcr.2004.05.023

Cited by 111 publications

(104 citation statements)

References 58 publications

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“…in which old serum was used on young cells and vice versa), eliminating the possibility for comparison between individual serum donors. Other studies have cultured human myoblasts, but these were all carried out in growth media containing foetal calf serum (Dellavalle et al 2007, Fulle et al 2005, Schäfer et al 2006, Jacquemin et al 2004.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Established myoblast cultures were passaged with trypsin (5mg/ml) after 4 days, replated, expanded for 5 population doublings (Jacquemin et al 2004), frozen in serum free medium (Cryo-SFM , Promocell, Germany) and stored in liquid N 2 . Cells were thawed, passaged once and plated in 96 well dishes at a density of 1250 cells/well for proliferation experiments and 5000 cells per well for differentiation experiments.…”

Section: Established Myoblast Culturesmentioning

confidence: 99%

“…Differentiation was also examined in serum free BM containing transferrin and insulin (SF; Jacquemin et al 2004). Two populations of cells were examined in these experiments from biopsies 5 and 6 (Table 1).…”

Section: Established Myoblast Culturesmentioning

confidence: 99%

“…There are several reports comparing the terminal differentiation (i.e. myotube formation and myosin heavy chain expression) in human myoblasts from young and old donors (Jacquemin et al 2004, Lorrenzon et al 2004, Beccaficco et al, 2007 that suggest impaired or delayed differentiation in old myoblasts. However, when comparing individual data from more than one young and one old donor these differences are less clear, with cells from many old individuals showing no reduction in myotube formation (Fulle et al, 2005, Mouly et al, 2005, Schäfer et al, 2006.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

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Sera from young and older humans equally sustain proliferation and differentiation of human myoblasts

George

Velloso

Alsharidah

et al. 2010

Experimental Gerontology

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Please cite this article as: George, Tomasz, Velloso, Cristiana P., Alsharidah, Mansour, Lazarus, Norman R., Harridge, Stephen D.R., Sera from young and older humans equally sustain proliferation and differentiation of human myoblasts, Experimental Gerontology (2010Gerontology ( ), doi: 10.1016Gerontology ( /j.exger.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Running title: Ageing serum and human satellite cell behaviour A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT AbstractUsing a human primary muscle cell culture model the behavior of myoblasts (satellite cells) cultured in human serum obtained from either young or elderly individuals was studied. Serum was obtained from a total of 13 young (7 male and 6 females aged, 23-36 years) and 9 elderly (4 male and 5 females aged 69-84 years) subjects and used in a number of experiments. Myoblasts were extracted from human muscle biopsy samples taken from the vastus lateralis. In the first experiment myoblasts were isolated immediately after extraction from the biopsy in media containing human sera to examine its effects on the onset and progression of Ki67 and desmin expression. No effect of the age of the serum was observed at 3, 5 or 7 days of proliferation. In addition, cells that had been expanded initially in optimum myoblast growth medium (GM, containing foetal calf serum and additional growth factors) prior to culture in medium containing 15% human serum were studied. The proportion of proliferating muscle cells co expressing desmin and Ki67 antigens after 46 hours was again similar in the young and old serum conditions. Culturing these myoblasts in media containing 2% human serum to study their fusion and differentiation also resulted in no difference between young and old serum conditions in terms of the percentage of nuclei inside myosin heavy chain positive myotubes. Despite the variability of different samples of myoblasts, the age of the serum has no affect on the expression of any measured index.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Established Myoblast Culturesmentioning

confidence: 99%

Section: Established Myoblast Culturesmentioning

confidence: 99%

Section: Accepted M Manuscriptmentioning

confidence: 99%

See 2 more Smart Citations

Sera from young and older humans equally sustain proliferation and differentiation of human myoblasts

George

Velloso

Alsharidah

et al. 2010

Experimental Gerontology

View full text Add to dashboard Cite

show abstract

“…10,11 The signals induced by IGF-I ultimately lead to increased proliferation, differentiation and fusion of myoblasts and myotube/myofiber hypertrophy. [12][13][14] Consequently, a basis exists for the administration of IGF-I to enhance muscle regeneration and promote functional recovery after injury. However, no study has directly compared different methods for increasing IGF-I levels during muscle regeneration.…”

Section: Introductionmentioning

confidence: 99%