IgE to recombinant allergens <scp>A</scp>pi m 1, <scp>V</scp>es v 1, and <scp>V</scp>es v 5 distinguish double sensitization from crossreaction in venom allergy

Müller, Ulrich; Schmid‐Grendelmeier, Peter; Hausmann, Oliver; Helbling, Arthur

doi:10.1111/j.1398-9995.2012.02847.x

Cited by 113 publications

(107 citation statements)

References 23 publications

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“…This most likely reflects difficulties in identification of the insects [18] as well as the fact that some of the reactions were not allergic ones. IgE to the major venom allergens rApi m 1, rVes v 1 and rVes v 5 have been shown to have a very high sensitivity and specificity when investigating DS patients [19,20,21,22]. Our study supports the value of adding IgE to major allergens in such patients, since double sensitization could only be confirmed in 10%.…”

Section: Discussionsupporting

confidence: 69%

Insect Sting Reactions and Specific IgE to Venom and Major Allergens in a General Population

Mosbech

Tang²,

Linneberg³

2016

Int Arch Allergy Immunol

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Background: Insect sting reactions are frequently reported, but population studies documenting the frequency and the relation to IgE-sensitization and serum tryptase are scarce. Methods: Questionnaire data and results from measurements of specific IgE against venom, major allergens and cross-reacting carbohydrate determinants (CCDs) were collected from 2,090 adult participants in a cross-sectional survey. Results: 13% of the population reported symptoms of sting reactions and about half were systemic in nature. In all, 15% were sensitized to venom but only 31% of these had reacted to stings and only 38% of those with reactions had IgE to venom. In addition, 12% with IgE to venom were double-sensitized (DS), i.e. to both bee and wasp venom. Among DS IgE to major venom allergens, rApi m 1, rVes v 1 and rVes v 5 were negative and of no help in 31%, but 59% could be identified as likely sensitized to bee or wasp. IgE to CCDs occurred in only 0.7%, but 80% of these were DS. Finally, 36% with IgE to CCDs had had symptoms, mostly local. Serum tryptase was not associated with a history of sting reactions. Conclusions: In a temperate climate, self-reported insect sting reactions and sensitization to venom are frequent, but in most cases, these are not seen in the same individual. In DS individuals, measurements of IgE to major allergens can be helpful in some but not all cases and additional analyses are needed. IgE to CCDs may have some clinical relevance.

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Section: Discussionsupporting

confidence: 69%

Insect Sting Reactions and Specific IgE to Venom and Major Allergens in a General Population

Mosbech

Tang²,

Linneberg³

2016

Int Arch Allergy Immunol

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“…Only 47% of 76 patients with double positivity to whole bee and wasp (yellow jacket) venoms reacted to recombinant species-specific major allergens (rSSMA) from both of these species. The specificity of IgE to rSSMA was excellent, especially for wasp venom [31]. In another study, component-resolved diagnosis with wasp-specific recombinant allergen components Ves v I and Ves v 5 was a reliable method of diagnosing wasp/yellow jacket allergy [32].…”

Section: Triggers and Mechanismsmentioning

confidence: 99%

World Allergy Organization Anaphylaxis Guidelines: 2013 Update of the Evidence Base

Simons

Ardusso

Dimov

et al. 2013

Int Arch Allergy Immunol

244

181

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The World Allergy Organization (WAO) Guidelines for the assessment and management of anaphylaxis are a widely disseminated and used resource for information about anaphylaxis. They focus on patients at risk, triggers, clinical diagnosis, treatment in health care settings, self-treatment in the community, and prevention of recurrences. Their unique strengths include a global perspective informed by prior research on the global availability of essentials for anaphylaxis assessment and management and a global agenda for anaphylaxis research. Additionally, detailed colored illustrations are linked to key concepts in the text [Simons et al.: J Allergy Clin Immunol 2011;127:593.e1-e22]. The recommendations in the original WAO Anaphylaxis Guidelines for management of anaphylaxis in health care settings and community settings were based on evidence published in peer-reviewed, indexed medical journals to the end of 2010. These recommendations remain unchanged and clinically relevant. An update of the evidence base was published in 2012 [Simons et al.: Curr Opin Allergy Clin Immunol 2012;12:389-399]. In 2012 and early 2013, major advances were reported in the following areas: further characterization of patient phenotypes; development of in vitro tests (for some allergens) that help distinguish clinical risk of anaphylaxis from asymptomatic sensitization; epinephrine (adrenaline) research, including studies of a new epinephrine auto-injector for use in community settings, and randomized controlled trials of immunotherapy to prevent food-induced anaphylaxis. Despite these advances, the need for additional prospective studies, including randomized controlled trials of interventions in anaphylaxis is increasingly apparent. This 2013 Update highlights publications from 2012 and 2013 that further contribute to the evidence base for the recommendations made in the original WAO Anaphylaxis Guidelines. Ideally, it should be used in conjunction with these Guidelines and with the 2012 Guidelines Update.

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“…On the other hand, studies in patients allergic to bee venom demonstrated that phospholipase A 2 , phosphomonoesterase and hyaluronidase are the major allergens present in apitoxin (Müller et al, 2012). Thus, the removal of phospholipase A 2 and other high molecular weight proteins could improve the product safety and allow the treatment of many diseases at low risk.…”

Section: Introductionmentioning

confidence: 99%

Fractionation of Apis mellifera venom by means of ultrafiltration: removal of phospholipase A 2

Brito

Bastos

Heneine

et al. 2018

Braz. J. Chem. Eng.

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-The fractionation of apitoxin (bee venom) by means of a commercial 10 kDa ultrafiltration membrane was investigated aiming at the removal of phospholipase A 2 , the main allergenic substance. The feed content was varied from 1 to 50 g apitoxin/L, in deionized water, and caused changes in membrane flux and rejection, due to concentration polarization. The increase in pressure difference and stirring rate improved the flux through the membrane. The best result was achieved for 1 g apitoxin/L in feed stream, with a pressure difference of 220 kPa, and 750 rpm, with a permeate flux of 103 kg/m 2 h. The use of ultrafiltration was efficient to improve the permeate safety since biological tests revealed that the remaining enzyme lost its ability to catalyze the hydrolysis of phospholipids.

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IgE to recombinant allergens Api m 1, Ves v 1, and Ves v 5 distinguish double sensitization from crossreaction in venom allergy

Abstract: Specificity of sIgE to rSSMA of both species is excellent. Sensitivity of sIgE to rSSMA was optimal for wasp venom. Sensitivity of bee venom Api m 1 could be increased by adding rSSMA of other important bee venom allergens.

Cited by 113 publications

References 23 publications

Insect Sting Reactions and Specific IgE to Venom and Major Allergens in a General Population

Insect Sting Reactions and Specific IgE to Venom and Major Allergens in a General Population

World Allergy Organization Anaphylaxis Guidelines: 2013 Update of the Evidence Base

Fractionation of Apis mellifera venom by means of ultrafiltration: removal of phospholipase A 2

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