IgE spécifiques et histamino-libération

Bernady, A.; Taytard, A; Lara, Jacinto

doi:10.1016/s0335-7457(96)80108-0

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…But HR is costly in terms of both materials (radioisotopes) and laboratory technician time. To date, the real benefit brought by HR remains controversial [3]. Thus, the aim of our study was to perform an easy flow cytometry test for activated basophil detection, without any previous cell separation steps, using a model consisting of whole blood without pretreatment (free of any additional mediators such as IL-3), with a short incubation time in order to provide a method with a reasonable degree of standardization which could be used in different laboratories.…”

Section: Discussionmentioning

confidence: 99%

“…Most in vitro tests are focused on basophils, as they are circulating cells (in contrast to mast cells). Nevertheless, technical difficulties such as the low number of circulating basophils, the necessity of leucocyte isolation and the difficulty of measuring very low levels of mediators make these methods unreliable and their diagnostic value controversial, as sensitivity and specificity remain to be clearly defined [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

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Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method

Monneret

Gutowski

Bienvenu

1999

Clinical and Experimental Immunology

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In the field of allergy diagnosis, most in vitro functional tests are focused on basophils. Nevertheless, the very small number of circulating basophils limits these experiments and their clinical benefit remains controversial. As flow cytometry is a valuable tool for identifying cell populations, even at low concentrations, we developed a tricolour flow cytometric method for the study of allergen-induced basophil activation. Identification of cells was based both on CD45 expression and on the presence of IgE on the cell surface, since basophils express high-affinity receptors for IgE (Fc epsilon RI). Cell activation upon allergen challenge was assessed by the expression of CD63 antigen on the plasma membrane. Basophil isolation and activation (with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine) were validated in 32 non-allergic patients. In 12 allergic patients, basophil stimulation by a relevant allergen was in most cases positive (10/12). Furthermore a concentration-dependent hook effect was observed. Of the allergic and non-allergic patients, none showed non-specific activation with an irrelevant allergen (specificity 100%). Overall, our preliminary results, even in a small population, suggest that this is a reliable and valuable method for the diagnosis of allergies complementing specific allergen IgE and skin test results. Obviously, additional clinical studies are needed to validate these first results.

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Section: Discussionmentioning

confidence: 99%