IgE reactivity to fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs

Imanishi, Ichiro; Uchiyama, Jumpei; Mizukami, Keijiro; Kamiie, Junichi; Kurata, K.; Fujimura, Masato; Shimakura, Kuniyoshi; Nishifuji, Koji; Sakaguchi, Masahiro

doi:10.21203/rs.2.19058/v2

Cited by 3 publications

(8 citation statements)

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“…The grocery store or the surrounding environment might be the source of these allergies [25]. In this study, 84 % were attributable to tuna, mackerel, and codfish allergies as well as in previous studies [26,27], seafood and animals are substantial sources of allergens for dogs. Interestingly, crab/shrimp is unlikely to cause allergies (Figure 5).…”

Section: Discussionsupporting

confidence: 67%

Detecting common allergens in dogs with atopic dermatitis in South Korean Provinces using a serological immunoglobulin E-specific allergen test

Adam

Park²,

Cho³

et al. 2022

Vet World

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Background and Aim: Canine atopic dermatitis (CAD) is a hereditary susceptibility to the development of allergic symptoms in response to repeated exposure to generally innocuous substances known as "allergens." Allergens can be plants, animals, mold, mites, or milk. At present, serological enzyme-linked immunoassay (ELISA) kits are used for immunoglobulin E (IgE)-specific allergen detection due to their simplicity and accuracy. This study aimed to detect allergens in dogs with CAD and determine how they differ according to season, breed, age, and sex using a serological test in six provinces in South Korea for 12 months. This will allow practitioners to easily understand the risk factors related to CAD. Materials and Methods: In this study, IgE allergen-specific ELISA kits were used. The allergens were detected in serum samples collected from different regions considering season, sex, breed, and age. Allergens were divided into the following Ten categories: 1. Dairy, yeast, and egg, 2. grains, 3. vegetables, 4. meat, 5. seafood, 6. animals, 7. mold, 8. insects, 9. mites, and 10. trees. Results: The percentage of allergens detected in males (54.8%) was higher than that of females (45.2%); 54.2% of allergens occurred in 3-year-old dogs or older. Moreover, regarding frequency, 65.6% of overall allergens occur during autumn; Chungcheongnam-do and Jeollabuk-do showed 20.7% and 20.9%, respectively. Additionally, among allergens categories, notable allergen occurrence was as follows: 38.3% corn; 28.7% potatoes; 22.7% duck; 24.4%,codfish; 31.2% animal wool; 95.6% Aspergillus fumigatus; 31.9% flea; 41.8% oak; and 25.0% sheep's sorrel grass. Conclusion: This study showcases the frequency of 60 allergens in six provinces detected in dogs with CAD; most likely from food or the environment using serological ELISA kits. Environmental sensitizer results can be considered for humans suffering from allergies to avoid a similar environment. A large-scale study can be performed to evaluate the allergens in the state. However, neither a skin test nor feed analysis was conducted, which is a limitation of this study.

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Section: Discussionsupporting

confidence: 67%

Detecting common allergens in dogs with atopic dermatitis in South Korean Provinces using a serological immunoglobulin E-specific allergen test

Adam

Park²,

Cho³

et al. 2022

Vet World

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“…Finally, we calculated A-RISC indices for the two chains of TPM, a well-known panallergen for humans and a recently found fish allergen in dogs. 8 As can be seen in Figure S1, the high-value indices suggested a substantial risk of cross-reactivity between mammal, poultry and fish TPMs. This risk also was important between the TPMs of these species and two inhalant allergens, Bla g 7 and Der f 10, the latter being a minor mite allergen for dogs.…”

Section: Resultsmentioning

confidence: 87%

“…Finally, we calculated A‐RISC indices for the two chains of TPM, a well‐known panallergen for humans and a recently found fish allergen in dogs 8 . As can be seen in Figure S1, the high‐value indices suggested a substantial risk of cross‐reactivity between mammal, poultry and fish TPMs.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of the theoretical risk of cross‐reactivity among recently identified food allergens for dogs

Olivry

O’Malley

Chruszcz

2022

Veterinary Dermatology

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Background: There is increasing evidence of cross-reactivity between allergens of close or distant species. The A-RISC (Allergens'-Relative Identity, Similarity and Cross-reactivity) index helps evaluate the risk of theoretical cross-reactivity between proteins of the same family among different species.Objectives: To report the A-RISC indices for several food allergens of dogs between multiple food sources. Materials and Methods:We selected several recently characterised food allergens for dogs from fish and chicken (ACTA1, ALDOA, CKM, ENO3, GAPDH, PKM and TPI1), fish (TPM1/2), beef/lamb (PGM1) and corn/potato (WAXY). When quality sequence data were available, A-RISC indices were calculated between multiple animal and plant species that can be used as food sources. For the TPM subunits, A-RISC indices also were calculated with the environmental allergens Bla g 4 and Der f 10, and the Toxocara canis nematode. Results:The A-RISC indices suggest a substantial theoretical risk of crossreactivity between species for all allergens considered. For TPM, this risk also extends to the environmental and nematode allergens. Conclusions and clinical relevance:There is a high theoretical risk of crossreactivity between allergens of different species used as food sources. The clinical relevance of these elevated A-RISC indices should be studied further.

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“…"sensitised dogs"). [11][12][13][14][15][16] Of all the allergens identified in the references above, only bovine serum albumin (ALB, Bos d 6) and three egg white proteins [ovomucoid (Gal d 1), ovalbumin (Gal d 2) and ovotransferrin (Gal d 3)] were identified in the serum isolated from a small number of dogs with food allergies proven by a positive oral challenge. Thus, there is a need to expand both this list of molecularly characterised food allergens and the number of sera collected from dogs with provocation-proven food allergies.…”

Section: Introductionmentioning

confidence: 99%

Identification of major and minor chicken allergens in dogs

Olivry

Pucheu‐Haston

Mayer³

et al. 2021

Veterinary Dermatology

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Background -Allergens targeted by serum-specific immunoglobulin E (sIgE) in dogs clinically allergic to chicken have not been reported.Objectives -To characterise the allergens targeted by sIgE in dogs sensitised and allergic to chicken.Animals -Sera from three dogs not sensitised to chicken, from 10 chicken sensitised dogs and from 12 chicken allergic dogs.Methods and materials -Enzyme-linked immunosorbent assay (ELISA) and immunoblotting with a commercial chicken extract were utilized. The bands identified on immunoblotting were sequenced by mass spectrometry for allergen characterization.Results -Using ELISA, we detected chicken-sIgE above the positive threshold in zero of three (0%) nonsensitised dogs, five of five (100%) chicken-sensitised dogs (a selection criterion), and in seven of 12 (58%) chickenallergic dogs. Immunoblotting performed with the same extract revealed IgE-bound protein bands in 100% of all chicken-sensitised and -allergic dogs, respectively. To identify the allergens, we excised the corresponding bands on the electrophoretic gel, and submitted them for sequencing by mass spectrometry. We conclusively identified seven major allergens (serum albumin, pyruvate kinase M, enolase 3, creatine kinase M, lactate dehydrogenase A, glyceraldehyde-3-phosphate dehydrogenase and triose-phosphate isomerase) and one minor allergen (troponin C), which are relevant to dogs.Conclusions and clinical relevance -We identified herein seven major chicken allergens for dogs, several of which are known to be cross-reactive allergens for humans. Based on their degree of sequence identity, these allergens exhibit the theoretical potential to be cross-reactive between poultry and mammalian meats; six of these allergens already are known to be cross-reactive between chicken and fish species. Future studies should address the clinical relevance and cross-reactivity potential of these chicken allergens in dogs.

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IgE reactivity to fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs

Cited by 3 publications

References 40 publications

Detecting common allergens in dogs with atopic dermatitis in South Korean Provinces using a serological immunoglobulin E-specific allergen test

Detecting common allergens in dogs with atopic dermatitis in South Korean Provinces using a serological immunoglobulin E-specific allergen test

Evaluation of the theoretical risk of cross‐reactivity among recently identified food allergens for dogs

Identification of major and minor chicken allergens in dogs

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