IgE‐reactivity profiles to allergen molecules in Russian children with and without symptoms of allergy revealed by micro‐array analysis

Abstract

Background: The analysis of longitudinal birth cohorts with micro-arrayed allergen molecules has provided interesting information about the evolution of IgE sensitization in children. However, so far no cross-sectional study has been performed comparing IgE sensitization profiles in children with and without symptoms of allergy. Furthermore, no data are available regarding molecular IgE sensitization profiles in children from Russia. Methods: We recruited two groups of age-and gender-matched children, one (Group 1: n = 103; 12.24 ± 2.23 years; male/female: 58/45) with symptoms and a second (Group 2: n = 97; 12.78 ± 2.23 years; male/female: 53/44), without symptoms of allergy according to international ISAAC questionnaire. Children were further studied regarding symptoms of allergy (rhinitis, asthma, atopic dermatitis) according to international guidelines, and skin prick testing with a panel of aeroallergen extracts was performed before sera were analyzed in an investigator-blinded manner for IgE specific to more than 160 micro-arrayed allergen molecules using ImmunoCAP ISAC technology. Results: IgE sensitization = or >0.3 ISU to at least one of the micro-arrayed allergen molecules was found in 100% of the symptomatic children and in 36% of the asymptomatic children. Symptomatic and asymptomatic children showed a comparable IgE sensitization profile; however, frequencies of IgE sensitization and IgE levels to the individual allergen molecules were higher in the symptomatic children. Aeroallergen sensitization was dominated by sensitization to major birch pollen allergen, Bet v 1, and major cat allergen, Fel d 1. Food allergen sensitization was due to cross-sensitization to PR10 pollen and food allergens whereas genuine peanut sensitization was absent.

The Molecular Allergen Recognition Profile in China as Basis for Allergen-Specific Immunotherapy

“…Of note, allergic patients may suffer from isolated allergic symptoms in certain organs (i.e., monomorbidity) or from various allergic comorbidities affecting several different organs at the same time and thus present with different allergic phenotypes which may also vary over time (2). The detailed analysis of the evolution of allergic sensitization from early childhood to adolescence suggests that allergic sensitizations occur early in life and, depending on genetic and environmental factors, in particular in response to repeated allergen contact, progress from clinically silent forms of IgE sensitizations which are characterized by the presence of low levels of allergen-specific IgE antibodies in the blood without accompanying symptoms towards symptomatic allergy (3,4). The analysis of molecular IgE sensitization profiles in birth cohorts has shown that asymptomatic IgE sensitization frequently precedes symptomatic allergy which then starts with mild symptoms such as allergic rhinitis and consecutively progresses to severe forms such as allergic asthma (5)(6)(7).…”

From Allergen Molecules to Molecular Immunotherapy of Nut Allergy: A Hard Nut to Crack

“…The sensitization to class II food allergens is initially caused by pollen allergens and results in IgE and T cell cross-reactivity with the related food allergens ( 35 , 36 ). IgE sensitization to class II food allergens is highly prevalent in countries with high exposure to the cross-reactive pollen allergens ( 37 , 38 ). Accordingly, diagnostics including the measurement of IgE against the originally sensitizing pollen allergens ( 39 ) and allergen-specific immunotherapy to the cross-reactive pollen allergens can improve not only pollen allergy but also the associated food allergy to some extent ( 40 , 41 ).…”

Comments on vitamin D and sensitization, asthma treatment, and lung function development

“…The role of dietary factors in allergy has been reported in several recent publications in PAI 5–7 ; in addition, the reader will find in recent publications investigations on the effect of vitamin D on the immune system, as well as on allergic diseases 8–10 . Additionally, you may find some interest to look at sensitization patterns reported recently in Northern Europe, and how they may differ from the Spanish study 11 …”

“…8-10 Additionally, you may find some interest to look at sensitization patterns reported recently in Northern Europe, and how they may differ from the Spanish study. 11…”