IgE Reactivity of Recombinant Pac c 3 from the Asian Needle Ant &lt;b&gt;&lt;i&gt;(Pachycondyla chinensis)&lt;/i&gt;&lt;/b&gt;

Precision allergy molecular diagnostic applications (PAMD@) is increasingly entering routine care. Currently, more than 130 allergenic molecules from more than 50 allergy sources are commercially available for in vitro specific immunoglobulin E (sIgE) testing. Since the last publication of this consensus document, a great deal of new information has become available regarding this topic, with over 100 publications in the last year alone. It thus seems quite reasonable to publish an update. It is imperative that clinicians and immunologists specifically trained in allergology keep abreast of the new and rapidly evolving evidence available for PAMD@. PAMD@ may initially appear complex to interpret; however, with increasing experience, the information gained provides relevant information for the allergist. This is especially true for food allergy, Hymenoptera allergy, and for the selection of allergen immunotherapy. Nevertheless, all sIgE tests, including PAMD@, should be evaluated within the framework of a patient's clinical history, because allergen sensitization does not necessarily imply clinical relevant allergies.

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“…Jeong et al recently recombinantly expressed Pac c 3 from needle ants and showed its cross-binding with Ves v 5 by ImmunoCAP 263. …”

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confidence: 99%

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Ansotegui¹,

Melioli²,

Canonica³

et al. 2020

World Allergy Organization Journal

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“…Neste sentido, cabe salientar que a reação de anticorpos bovinos específicos evidenciou uma maior reatividade à rBm86-CG obtida com sulfato de amônio em comparação a acetona e metanol. Além disso, a precipitação por sulfato de amônio permite que a amostra seja concentrada e submetida à purificação por cromatografia sem prejuízos a sua imunogenicidade (JEONG et al, 2016;KWON, 2012;LI et al, 2015;MA, 2013).…”

Section: Resultados E Discussão Precipitaçãounclassified

Avaliação De Métodos Para Obtenção De Proteínas Recombinantes

Santos¹,

Piraine²,

Cunha³

et al. 2017

SCI. ANIM. HEALTH

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RESUMOutilização de Pichia pastoris para a produção de proteínas recombinantes apresenta inúmeras vantagens que esse sistema agrega, destacando-se a exportação das moléculas para o meio extracelular. Isto permite que a obtenção das proteínas possa ser realizada por métodos de precipitação, com uso de sais neutros ou solventes orgânicos, sem trazer prejuízos. O objetivo deste trabalho foi avaliar a utilização de sulfato de amônio, acetona e metanol para obtenção da proteína recombinante Bm86 do carrapato Rhipicephalus microplus expressa por P. pastoris. A ação provocada pelos solventes orgânicos ocasionou uma solubilidade parcial das proteínas. Na quantificação por densitometria da proteína rBm86-CG, verificou-se que utilizando acetona (1:2) foi obtido 30,3 mg/L, metanol (1:2) 60,5 mg/L e com sulfato de amônio a 70% (83,5 mg/L). A manutenção das características antigênicas após a precipitação foi verificada pela técnica de Western Blot, sendo que a proteína rBm86-CG obtida pelos três métodos foi reconhecida por anticorpos específicos. Os resultados sugerem que a precipitação com sais neutros e solventes orgânicos pode ser utilizada para obtenção de proteínas recombinantes como a rBm86-CG, sem trazer prejuízos a sua antigenicidade.Palavras-chave: Pichia pastoris. Sais neutros. Solventes orgânicos. Antigenicidade.A

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“…Na-ASP-2, a candidate hookworm vaccine, belongs to this family and its administration was associated with generalized urticarial reactions in several volunteers [26]. Cross-reactivity has been reported between Ag5/CAP family members with moderate sequence conservation; this is the case of Pac c 3, a Chinese ant allergen, and Ves v 5, a relevant wasp allergen, which share 50% sequence identity [27]. It is worth mentioning that a lack of cross-reactivity has also been reported for Sol i 3 and Ves v 5, which share 44% sequence identity, but with limited conservation of surface chemical properties and topology [28].…”

Section: Discussionmentioning

confidence: 99%

“…For most Ag-5 related members, production in eukaryotic hosts has been preferred due to their high content of disulfide bridges [27, 28]. It is possible that sensitization rates are underestimated; however, since PU is considered a cellular mediated disease, it is expected that the repertoire of linear T-cell epitopes of this recombinant product is completely representative of the natural molecule.…”

Section: Discussionmentioning

confidence: 99%

Cellular and Humoral Responses to Cte f 2, a Cat Flea Allergen, in Children with Papular Urticaria

Sabogal

Lozano

Mercado

et al. 2019

Int Arch Allergy Immunol

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Background: Papular urticaria (PU) is a common insect bite skin hypersensitivity in tropical countries. In order to gain insight into its causal allergens, we aimed to evaluate cellular and humoral immune responses to the recombinant salivary antigen Cte f 2 from the cat flea Ctenocephalides felis. Method: Sixty patients with PU and 27 healthy controls were included in this study. Specific IgE, IgG, IgG1, and IgG4 against Cte f 2 and C. felis extract were determined by ELISA. The T-cell response was analyzed using a carboxyfluorescein succinimidyl ester (CFSE)-based dilution assay and Th1/Th2/Th17 cytokine measurements. In addition, a proteomic analysis of IgG and IgE reactive spots of C. felis extract was performed. Results: The frequency of IgE sensitization to Cte f 2 was similar between patients (36.7%) and controls (40.7%). The specific IgE, IgG1, and IgG4 responses to Cte f 2 and C. felis extract were not significantly different between patients and controls. Among the 3 conditions (i.e., Cte f 2, C. felis extract, and only medium) Cte f 2 was the strongest inducer of CD3+CD4+ proliferation in the patients; however, the mean response was not significantly different from those in controls (Cte f 2: 4.5 vs. 2.5%; p = 0.46). No salivary proteins were identified in C. felis, and most of the spots were identified as muscle-skeletal components (tropomyosin, actin, myosin, and ankirin). Conclusions: Cte f 2 induces IgE and IgG production as well as T-cell proliferation in children living in a geographical area where PU induced by a flea bite is common. The use of C. felis extract is not recommended for the study of bite-induced hypersensitivity disease since salivary antigens are not well represented.

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IgE Reactivity of Recombinant Pac c 3 from the Asian Needle Ant <b><i>(Pachycondyla chinensis)</i></b>

Cited by 20 publications

References 27 publications

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Avaliação De Métodos Para Obtenção De Proteínas Recombinantes

Cellular and Humoral Responses to Cte f 2, a Cat Flea Allergen, in Children with Papular Urticaria

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