IgE Mediated Histamine Release: &lt;i&gt;in vitro&lt;/i&gt; Separation into two Phases

Lichtenstein, Lawrence M.; Raymundo, Bernardo

doi:10.1159/000230488

Cited by 26 publications

(12 citation statements)

References 5 publications

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“…This self-limiting process can be demonstrated in another way. Stimulation of the basophil in the absence of extracellular calcium results in no mediator secretion [11]. If this condition is maintained for 45–60 min and calcium is returned to the reaction, no secretion occurs (compared to simply incubating the cells in the absence of extracellular calcium).…”

Section: Ige-mediated Signalingmentioning

confidence: 99%

Self-Termination/Anergic Mechanisms in Human Basophils and Mast Cells

MacGlashan¹

2009

Int Arch Allergy Immunol

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All cellular reactions include feedback loops, both positive and negative. Following all receptor-mediated stimulation there are events that activate the cell response and events that dampen the receptor response back to resting conditions. IgE-mediated activation of mast cells and basophils necessarily includes a variety of signaling events that serve to terminate the activation processes. Some of these negative feedback loops are active even in resting cells and likely serve to establish a set point for the cell’s response to IgE-mediated stimulation. But many negative feedback loops become active only after the cell is stimulated. By and large, it will be these processes that will be discussed in this review.

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Section: Ige-mediated Signalingmentioning

confidence: 99%

Self-Termination/Anergic Mechanisms in Human Basophils and Mast Cells

MacGlashan¹

2009

Int Arch Allergy Immunol

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“…When the two stages of histamine release were studied, cells were incubated with A g E or a-IgE for 2 min in the absence of C a 2+ and M g2*, washed twice with cold P A , and resuspended in P A C M as pre viously described [12,17], Histamine was assayed by an automated fluorometric technique devel oped by Siraganian [26].…”

Section: Histamine Releasementioning

confidence: 99%

IgE-Mediated Histamine Release from Human Basophils: Differences between Antigen E- and Anti-IgE-Induced Secretion

Marone¹,

Kagey‐Sobotka²,

Lichtenstein³

1981

Int Arch Allergy Immunol

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The mechanism of the IgE-mediated release of histamine from human basophils differs when the process is initiated by antigen as compared to anti-IgE (a-IgE). Antigen causes release from the basophils of different individuals over a wide range of concentrations, while release to a-IgE occurs over a limited concentration range; antigen excess inhibition is also relatively slight as compared to that observed with a-IgE. Release by antigen occurs with a shorter lag period and a more rapid rate than seen with a-IgE· Agonists which inhibit release by acting through adenylate cyclase (dimaprit, adenosine, isoproterenol) are significantly more potent with respect to antigen than to a-IgE-induced release. Other agonists (3-isobutyl-1-methylxanthine, dibutyryl cyclic AMP) which increase cyclic AMP by different mechanisms are equally effective against antigen and a-IgE, suggesting that the receptor-adenylate cyclase interactions differ between the two stimuli. The cyclooxygenase inhibitor, indomethacin, consistently enhances antigen- but not a-IgE-induced release while cytochalasin B, which acts in part on microfilaments, enhances both processes equally. These data indicate that cross-linking of surface antibody IgE by antigen initiates a release process different from that caused by a-IgE, perhaps because the former is a multivalent ligand and the latter is functionally bivalent.

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“…It was demonstrated in the early 1970s that it was possible to accomplish this outcome by excluding extracellular calcium during a period of exposure to antigen. 17 This form of desensitization has been studied many different ways during the intervening years. A second method has been to perform the experiment under normal secretory conditions but to titrate in the antigen slowly, beginning with concentrations that induce only very weak secretion.…”

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confidence: 99%