IgE‐mediated antigen presentation

Mudde, Geert C.; Bheekha, Roy; Bruijnzeel-Koomen, Carla A.F.M.

doi:10.1111/j.1398-9995.1995.tb01132.x

Cited by 41 publications

(15 citation statements)

References 55 publications

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“…with fish, egg or milk. This finding supports the view that allergen-induced mast cell triggering may induce subsequent sensitization to antigens that happen to be near (Mudde et al, 1990;Aalberse, 1991;Santamaria et al, 1993;Mudde et al, 1995).…”

Section: Antigen or Allergensupporting

confidence: 86%

Food allergens

Aalberse

1997

Environmental Toxicology and Pharmacology

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A food allergen may be defined as a substance that reacts with IgE antibodies, induces allergic sensitisation or induces allergic reactions. Some allergens only induce allergic sensitisation but do not provoke symptoms, while others bind IgE but do not induce mast cell degranulation. There is no common structure that can predict whether a given antigen may be a strong food allergen. A complete food allergen, e.g. fish parvalbumin, is capable of stimulating the immune system to produce IgE antibodies, and degranulate mast cells upon subsequent contact. The reason(s) for why some patients with IgE to ovalbumin tolerate eggs, and why some react on one occasion but not on another, are mostly unclear, but may be related to changes in gut permeability induced by other food substances or by gastro-intestinal inflammation prior to the allergen contact. IgE antibodies to fruit or vegetables often show cross-reactivity, due to carbohydrate structures. These cross-reactive glycans have been designated cross-reactive carbohydrate determinants (CCD). Anti-CCD antibodies are highly cross-reactive. The antibodies do not have clinical significant because CCD-containing foods are usually well-tolerated by patients with IgE antibodies to CCD. These IgE antibodies may cause confusion in relation to allergy diagnosis. © 1997 Elsevier Science B.V.

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Section: Antigen or Allergensupporting

confidence: 86%

Food allergens

Aalberse

1997

Environmental Toxicology and Pharmacology

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“…We considered the possibility that these cells might contribute to the observed differences of the proliferative response between the native allergen and allergoid. It has been reported that antigen presentation via allergen–specific IgE bound to FcεRI is involved in T cell stimulation [47, 48, 49, 50]. Since the PBMC in our study were obtained from allergic subjects, allergen uptake via IgE/FcεRI is highly probable.…”

Section: Discussionmentioning

confidence: 93%

Reactivity of T Cells with Grass Pollen Allergen Extract and Allergoid

Kähler¹,

Stüwe²,

Cromwell³

et al. 1999

Int Arch Allergy Immunol

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Background: Successful allergen–specific immunotherapy is achieved with progressively increasing doses of allergen or allergoid. In order to gain further insight into the mechanism of action of allergoids several in vitro investigations were conducted. Methods: Peripheral blood mononuclear cells (PBMC) from grass pollen allergic and nonallergic subjects were stimulated with either grass pollen extract or allergoid and the proliferation and cytokine production (IL–5, IFN–γ) were measured. Similar investigations were performed with Phl p 5–specific T cell lines (TCL) and clones (TCC). Dendritic cells and PBMC were compared in terms of their relative efficacies as antigen–presenting cells. Results: Both allergen and allergoid induced proliferation and Th2 and Th1 cytokine synthesis by PBMC of allergic subjects, whereas PBMC of nonallergic subjects did not produce IL–5. The maximum level of IL–5 was obtained with a lower concentration than was necessary for maximal IFN–γ production. Higher stimulation doses of allergen and allergoid shifted the cytokine profiles towards a Th1 phenotype. TCL and TCC clearly showed reactivity with both allergen and allergoid when using autologous PBMC for antigen presentation, but compared with the native allergen the reactivity of the allergoid was reduced with most of the TCC. Using dendritic cells for antigen presentation a pronounced increase of stimulation of the TCC especially for the allergoids becomes obvious. Conclusion: In common with grass pollen allergen the corresponding allergoids possess a strong allergen–specific T cell–stimulating capacity. However, the degree of T cell stimulation by the allergoid seems to be dependent on the type of the antigen–presenting cell. Both, allergen and allergoid, can modulate T cell responses in a dose–dependent manner.

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“…Such IgE-expressing dendritic cells are found in lesional skin biopsies of both dogs 26 and humans 30,31 with AD, and it is thought that IgE at the surface of dendritic cells are involved in both allergen capture and focused presentation. 32,33 Of interest is the lack of observation of IgE + dendritic cells at sites challenged with flea saliva in flea-sensitized dogs. This difference between mite and flea allergen challenges might be caused by the chemical composition of the allergen(s) used or, possibly, to a variable immune response to allergens that are naturally found on the skin surface (mites) or injected directly into the dermis (flea saliva).…”

Section: Patch Testing In Hypersensitive Beagle Dogsmentioning

confidence: 99%

Patch testing of experimentally sensitized beagle dogs: development of a model for skin lesions of atopic dermatitis

Olivry

Deangelo

Dunston

et al. 2006

Veterinary Dermatology

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In humans with atopic dermatitis (AD), the epicutaneous application of allergens (atopy patch tests or APT) to which the patients are sensitized often results in the development of inflammation resembling that of spontaneous skin lesions. Dogs are affected with a natural homologue of human AD, but information on the induction of positive patch testing reactions is limited. The objectives of this pilot study were to determine the nature and cellular dynamics of inflammation occurring after APT in dogs hypersensitive to house dust mite and flea allergens. Laboratory Beagles were sensitized experimentally to Dermatophagoides farinae house dust mites (two dogs), Ctenocephalides felis flea saliva (one dog) or both (two dogs). Two other dogs served as nonsensitized controls. Both allergens and saline were applied epicutaneously. Macroscopic evaluations and skin biopsies were performed at 4, 24, 48 and 96 h after starting allergenic challenge. Biopsies were evaluated histologically and immunohistochemically with a panel of monoclonal antibodies specific for canine leucocyte antigens. Positive macroscopic reactions consisted of erythema, oedema and induration, and they occurred between 24 and 96 h after allergen application. Macroscopic and microscopic APT reactions developed only whenever serum IgE was present against tested allergens. Microscopically, positive APT was associated with epidermal hyperplasia, Langerhans' cell hyperplasia, and eosinophil and lymphocyte epidermotropism. Dermal inflammation was mixed and arranged in a superficial perivascular to interstitial pattern. Numerous IgE+-CD1+ dendritic cells and gamma-delta T-lymphocytes were observed. Macroscopically and microscopically, APT reactions in these experimentally sensitized animals resembled those seen in lesional biopsy specimens of dogs and humans with spontaneous AD. Therefore, APT in hypersensitive dogs provides a relevant experimental model to investigate the pathogenesis and treatment of both canine and human AD skin lesions.

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IgE‐mediated antigen presentation

Cited by 41 publications

References 55 publications

Food allergens

Food allergens

Reactivity of T Cells with Grass Pollen Allergen Extract and Allergoid

Patch testing of experimentally sensitized beagle dogs: development of a model for skin lesions of atopic dermatitis

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