IgE, IgG1, and IgG4 Reactivity to<i>Dermatophagoides pteronyssinus</i>Glycosylated Extract in Allergic Patients

Resende, Rafael de Oliveira; Ynoue, Leandro Hideki; Miranda, Juliana Silva; Almeida, Karine Cristine de; Silva, Deise Aparecida de Oliveira; Sopelete, Mônica Camargo; Alves, Ronaldo; Gennari-Cardoso, Margareth Leitão; Taketomi, Ernesto Akio

doi:10.1155/2019/9840890

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…To evaluate the inhibition of S2 against extract S1, competitive inhibition ELISA was conducted as previously described ( 23 ). High-affinity microtiter plates were loaded with extract S1 (1 μg/well) in carbonate-bicarbonate buffer, and plates were then blocked with PBS-T containing 1% BSA for 1 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients

Correa,

Miranda,

Oliveira

et al. 2023

Braz J Med Biol Res

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Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethyl-ligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2-specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non-atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed that carboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy.

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Section: Methodsmentioning

confidence: 99%

Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients

Correa,

Miranda,

Oliveira

et al. 2023

Braz J Med Biol Res

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“…A wheal diameter ≥ 3 mm after 15 min was considered positive. Serum samples were used to detect D. farinae-specific IgE by an indirect ELISA [30]. Briefly, high-affinity microplates were coated with 40 µg/mL D. farinae extract for 18 h at 4 • C and blocked with phosphate-buffered saline/0.05% Tween (PBS-T) with 1% Bovine Serum Albumin (BSA).…”

Section: Spt With Airborne Allergens and D Farinae-specific Ige Elisamentioning

confidence: 99%

Distinct Adverse Reactions to mRNA, Inactivated Virus, and Adenovirus Vector COVID-19 Vaccines: Insights from a Cohort Study on Atopic and Non-Atopic Subjects in Brazil

Oliveira,

Correa,

de Jesus

et al. 2024

Vaccines

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The emergence of COVID-19 caused by SARS-CoV-2 prompted an unprecedented global response to develop vaccines at an accelerated pace. Messenger RNA (mRNA) and adenovirus vector vaccines emerged as the frontrunners in global immunization efforts, significantly reducing hospitalization, severity, and mortality, supplemented by inactivated virus-based vaccines in developing countries. However, concerns regarding adverse effects, including allergic reactions, have been raised. This study aimed to investigate the adverse effects following COVID-19 vaccination, particularly in atopic and non-atopic individuals. A cohort of 305 volunteers receiving BNT162, ChAdOx1, or CoronaVac vaccines were assessed based on a Skin Prick Test (SPT), specific IgE levels, and clinical history of asthma and rhinitis. Adverse effects were self-reported and scored across the different vaccination shots. The results indicated a notable presence of mild adverse effects following the first and third doses, regardless of vaccine type. ChAdOx1 recipients experienced more adverse effects compared to those receiving BNT162 and CoronaVac, including headaches, muscle pain, fever, chills, nausea, and flu-like symptoms. Atopic individuals receiving ChAdOx1 reported more adverse effects, such as muscle pain, fever, and chills, compared to non-atopic individuals. Conversely, headaches were more frequently reported in non-atopic individuals receiving BNT162 compared to atopic individuals. No anaphylaxis or allergic reactions were reported, indicating valuable evidence supporting the safety of COVID-19 vaccination in individuals with respiratory allergies. This study highlights the importance of understanding vaccine-related adverse effects, particularly in vulnerable populations, to inform vaccination strategies and address safety concerns in global immunization campaigns.

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“…Dermatophagoides pteronyssinus (DP), a widely popular dominant house dust mite (HDM) which generally known as strong inducers of IgE antibody response (Liao et al 2015), has been identified to have many IgE-binding components in the allergen extract (Resende et al 2019). Allergy is mainly caused by Der p 1 and Der p 2 allergens and these allergens are recognized by more than 80% of atopic individuals (Cui et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus

et al. 2020

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Abstract. Adji A, Niode NJ, Memah VV, Posangi J, Wahongan GJP, Tallei TE. 2021. Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus. Biodiversitas 22: 72-78. Gene polymorphisms in Dermatophagoides pteronyssinus (DP) mite can affect the immune response that plays a role in atopic disease. This study aimed to determine the polymorphisms of Der p 1 gene from DP. House dust containing DP was used as a source of DNA containing the Der p 1 gene. The gene was amplified using specific primers DP Der 1 P 108F and DP Der 1 P 1509R. The amplified fragments were cloned using the Toyobo pTA2 vector and sorted then compared with the Gene Bank database. The result shows that there are 8 polymorphism sites in the Der p 1 gene positioned at 190 (GR), 463 (CT), 645 (AR), 751 (CY, CT), 787 (CG), 827 (TA; TW), and 1084 (CG). In the Der p 1 gene, there are 5 silent mutations and 3 nonsynonymous substitutions, resulting in amino acid polymorphisms as follows: Histidine H146 to Tyrosine Y146, Alanine A220 to Valine V220 and amino acid X220, and Tyrosine T232 to Serine S232. Polymorphisms in these amino acids lead to a polarity change, from polar to polar and non-polar to non-polar. These dynamics in polarity does not change the structure or function of the allergen protein. So that they can be used for vaccine design strategies.

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IgE, IgG1, and IgG4 Reactivity toDermatophagoides pteronyssinusGlycosylated Extract in Allergic Patients

Cited by 4 publications

References 40 publications

Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients

Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients

Distinct Adverse Reactions to mRNA, Inactivated Virus, and Adenovirus Vector COVID-19 Vaccines: Insights from a Cohort Study on Atopic and Non-Atopic Subjects in Brazil

Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus

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