IgE, IgG1, and IgG4 antibody responses to <i>Blomia tropicalis</i> in atopic patients

Pereira, E. A. L.; Silva, D. A. O.; Cunha-Júnior, Jair P.; Almeida, Karine Cristine de; Alves, Ronaldo; Sung, Sun‐Sang J.; Taketomi, Ernesto Akio

doi:10.1111/j.1398-9995.2005.00738.x

Cited by 42 publications

(50 citation statements)

References 15 publications

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“…These results were similar to those of our previous studies carried out in another group of AR patients from the same region (8), confirming that the high rate of this concurrent sensitization has certainly complicated the evaluation of the role of B. tropicalis in these patients, particularly in tropical and subtropical regions where both mites coexist.…”

Section: Discussionsupporting

confidence: 82%

“…In the present study, we demonstrated that there was an important IgE cross-reactivity with the Dp extract even when using the Bt-Con-A extract, suggesting that high-molecular weight allergens could also be involved in this cross-reactivity. Accordingly, in our previous studies (8), AR patients appeared to be preferentially sensi-tized to B. tropicalis high-molecular weight allergenic components as compared to lowmolecular weight allergens. Immunoblotting inhibition studies should be performed using the Bt-total and Bt-Con-A extracts to better characterize the components that are crossreactive and non-cross-reactive with Dp.…”

Section: Discussionmentioning

confidence: 99%

“…To evaluate the degree of cross-reactivity between Bt and Dp extracts, competitive inhibition assays were performed as previously described (8). Briefly, Bt-total, BtCon-A and Dp-total extracts were serially diluted 10-fold (10 2 to 10 -4 µg/mL) and preincubated overnight at 4ºC with six pooled sera (diluted 1:5) obtained from patients with high levels of specific antibodies to B. tropicalis.…”

Section: Competitive Inhibition Elisamentioning

confidence: 99%

“…IgE-, IgG1-, and IgG4-ELISA were performed as described elsewhere (8). Briefly, microtiter plates were coated with 1 µg/well Bt-total or Bt-Con-A extracts, blocked with PBS containing 0.05% Tween 20 and 1% bovine serum albumin, and then incubated with serum samples diluted 1:2 (IgE) or 1:5 (IgG1 and IgG4).…”

Section: Measurement Of Ige Igg1 and Igg4 By Elisamentioning

confidence: 99%

“…Total extracts of Bt and Dp were obtained from mite bodies provided by Dr. Federico Montealegre, Ponce School of Medicine, Porto Rico, USA, as described elsewhere (8). Briefly, 5 g dried mite powder was triturated in liquid nitrogen to disrupt the mites and allergens were extracted overnight at 4ºC in 50 mL 5 mM borate-buffered saline, pH 8.0, containing protease inhibitors (50 µg/mL leupeptin, 1.6 mM phenylmethylsulfonyl fluoride, 1 mM benzamidine, and 10 µg/mL aprotinin).…”

Section: Mite Extractsmentioning

confidence: 99%

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Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients

Almeida¹,

Silva²,

Gennari-Cardoso³

et al. 2006

Braz J Med Biol Res

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Blomia tropicalis (Bt) and Dermatophagoides pteronyssinus (Dp) are the prevalent house dust mites in tropical countries and are associated with allergic diseases. Glycosylated antigens are highly immunogenic and involved in different pathologies. We evaluated the presence of IgE, IgG1, and IgG4 to concanavalin A-binding antigens (Bt-Con-A) isolated from Bt-total extract in sera of allergic and non-allergic subjects. Bt-total and Bt-Con-A extracts were evaluated by SDS-PAGE and ELISA for reacting with IgE, IgG1, and IgG4 in sera of 121 patients with allergic rhinitis and 36 non-allergic individuals. All subjects were skin prick tested with Bt-total extract and inhibition tests were performed for IgE, IgG1, and IgG4 using both extracts (Bttotal and Bt-Con-A). Skin prick test showed that 58% of the patients were sensitized to Bt (Bt+), with 52% reactive to both mites (Bt and Dp) and 6% to Bt only. A broad spectrum of proteins (14-152 kDa) was visualized in Bt-total and components >27 kDa for the Bt-Con-A extract. ELISA showed a similar profile of IgE, IgG1 and IgG4 levels in response to Bt-total and Bt-Con-A extracts in different groups, although Bt+ patients showed a lower IgG4 reactivity to Bt-Con-A extract. Specific IgG1 levels were higher in Bt+ patients than in control subjects, and IgG4 levels showed no significant difference among groups. ELISA inhibition showed a partial IgE and total IgG1 and IgG4 cross-reactivity with Dp extract for Bt-total and Bt-Con-A extracts. We conclude that Con-A-binding components isolated from Bt constitute major allergens and are involved in both allergen sensitization (IgE response) and homeostasis maintenance (IgG1 and IgG4 responses).

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Competitive Inhibition Elisamentioning

confidence: 99%

Section: Measurement Of Ige Igg1 and Igg4 By Elisamentioning

confidence: 99%

Section: Mite Extractsmentioning

confidence: 99%

See 3 more Smart Citations

Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients

Almeida¹,

Silva²,

Gennari-Cardoso³

et al. 2006

Braz J Med Biol Res

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TLR9 agonist adsorbed to alum adjuvant prevents asthma-like responses induced by Blomia tropicalis mite extract

Nunes¹,

Alberca²,

Gomes³

et al. 2019

Journal of Leukocyte Biology

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Blomia tropicalis mite is highly prevalent in tropical and subtropical regions and it is associated with allergic diseases such as rhinitis and asthma. By using an OVA‐model of allergic lung disease, we have previously shown that sensitization in the presence of toll like receptors (TLRs) agonists attenuates subsequent OVA‐induced allergic responses. Here, we evaluated the effect of CpG‐ODN, a specific synthetic TLR‐9 agonist, on the development of experimental asthma induced by Blomia tropicalis extract, a relevant source of aeroallergens. Among different protocols of Blomia tropicalis extract sensitization, the subcutaneous sensitization in the presence of alum adjuvant induced the highest Th2 responses, including high IgE levels. Adsorption of CpG to Blomia tropicalis extract/Alum attenuated the airway hyperreactivity, the infiltration of inflammatory cells including eosinophils, and the IL‐5 content in BAL. In addition, lung peribronchial inflammatory infiltrate, mucus production and IL‐5‐producing CD3+CD4+ T cells were significantly reduced in the Blomia tropicalis extract/Alum+CpG group. Importantly, CpG inhibited total IgE production as well as active systemic or cutaneous anaphylaxis reactions. Inhibition of pulmonary Th2 responses was associated with increased IL‐10 production but not with IFN‐γ production. Notably, in IL‐10‐deficient mice, sensitization with OVA/Alum+CpG resulted in intense lung neutrophilia and IFN‐γ production, indicating that IL‐10 is necessary to inhibit subsequent Th1 immunity. Our work highlights the mechanisms of allergy attenuation by CpG and it indicates the potential use of Alum‐based formulation with CpG to treat allergic processes.

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Identifications of inhibitors of IgE production by human lymphocytes isolated from ‘Cha Chuukanbohon Nou 6’ tea leaves

Honma

Tagashira

Kanda

et al. 2009

J. Sci. Food Agric.

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IgE, IgG1, and IgG4 antibody responses to Blomia tropicalis in atopic patients

Cited by 42 publications

References 15 publications

Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients

Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients

TLR9 agonist adsorbed to alum adjuvant prevents asthma-like responses induced by Blomia tropicalis mite extract

Identifications of inhibitors of IgE production by human lymphocytes isolated from ‘Cha Chuukanbohon Nou 6’ tea leaves

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