IgE‐expressing memory B cells and plasmablasts are increased in blood of children with asthma, food allergy, and atopic dermatitis

Heeringa, Jorn J.; Rijvers, Liza; Arends, Nicolette; Pasmans, Suzanne G.M.A.; Dongen, Jacques J. M. van; Jongste, Johan C. de; Zelm, Menno C. van

doi:10.1111/all.13421

Cited by 62 publications

(93 citation statements)

References 27 publications

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“…B cells play a crucial role in the sensitization and adaptive immune response of asthma (Table ) . Admyre et al concluded that B cell–derived exosomes play a role in stimulating the allergic immune response.…”

Section: Resultsmentioning

confidence: 99%

Extracellular vesicles and asthma: A review of the literature

Sangaphunchai

Todd

Fairclough

2020

Clin Experimental Allergy

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Asthma is a chronic, recurrent and incurable allergy‐related respiratory disease characterized by inflammation, bronchial hyperresponsiveness and narrowing of the airways. Extracellular vesicles (EVs) are a universal feature of cellular function and can be detected in different bodily fluids. Recent evidence has shown the possibility of using EVs in understanding the pathogenesis of asthma, including their potential as diagnostic and therapeutic tools. Studies have reported that EVs released from key cells involved in asthma can induce priming and activation of other asthma‐associated cells. A literature review was conducted on all current research regarding the role and function of EVs in the pathogenesis of asthma via the PRISMA statement method. An electronic search was performed using EMBASE and PubMed through to November 2018. The EMBASE search returned 76 papers, while the PubMed search returned 211 papers. Following duplicate removal, titles and abstracts were screened for eligibility with a total of 34 studies included in the final qualitative analysis. The review found evidence of association between the presence of EVs and physiological changes characteristic of asthma, suggesting that EVs are involved in the pathogenesis, with the weight of evidence presently favouring deleterious effects of EVs in asthma. Numerous studies highlighted differences in exosomal contents between EVs of healthy and asthmatic individuals, which could be employed as potential diagnostic markers. In some circumstances, EVs were also found to be suppressive to disease, but more often promote inflammation and airway remodelling. In conclusion, EVs hold immense potential in understanding the pathophysiology of asthma, and as diagnostic and therapeutic markers. While more research is needed for definitive conclusions and their application in medical practice, the literature presented in this review should encourage further research and discovery within the field of EVs and asthma.

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Section: Resultsmentioning

confidence: 99%

Extracellular vesicles and asthma: A review of the literature

Sangaphunchai

Todd

Fairclough

2020

Clin Experimental Allergy

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“…Exponential advances in microarrays, time-of-flight mass cytometry (CyTOF), basophil activation tests, next-generation gene sequencing, and RNA-seq are generating huge enabling datasets. 15,115,116,[119][120][121][122][123] The risk that small populations of highly pathogenic cells might be masked by abundant signatures of more frequent or more active cells is combated by the emergence of enhanced single B-and T-cell immunophenotyping using flow cytometry-based assays. This allows longitudinal immunoprofiling of relevant cell subsets in individuals before and during AIT.…”

Section: Precision Medicine and Immune Monitoring Of Allergic Diseasesmentioning

confidence: 99%

Future research trends in understanding the mechanisms underlying allergic diseases for improved patient care

Breiteneder

Diamant

Eiwegger

et al. 2019

Allergy

101

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This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. AbstractThe specialties of allergy and clinical immunology have entered the era of precision medicine with the stratification of diseases into distinct disease subsets, specific diagnoses, and targeted treatment options, including biologicals and small molecules.This article reviews recent developments in research and patient care and future trends in the discipline. The section on basic mechanisms of allergic diseases summarizes the current status and defines research needs in structural biology, type 2 inflammation, immune tolerance, neuroimmune mechanisms, role of the microbiome and diet, environmental factors, and respiratory viral infections. In the section on diagnostic challenges, clinical trials, precision medicine and immune monitoring Zuzana Diamanthttps://orcid.org/0000-0003-0133-0100Thomas Eiwegger https://orcid.org/0000-0002-2914-7829Wytske J. Fokkens https://orcid.org/0000-0003-4852-229X

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“…The CD4 + CD25+CD127‐ phenotype specifically distinguishes human Treg, and these cells can be quantified in allergic patients with increases following SLIT for grass pollen and AIT in HDM‐allergic individuals . Furthermore, chemokine receptor expression patterns correlate strongly with Th cell function and can be used to identify Th1 (CCR6‐CXCR3 + CCR4‐), Th2 (CCR6‐CXCR3‐CCR4+) and Th17 (CCR6 + CXCR3‐CCR4+) subsets within CD4 memory cell populations (CD4 + CD45RA‐) . Grass pollen immunotherapy was shown to reduce the percentage of CCR3 + CD4+ cells in fresh PBMC of allergic rhinitis patients to levels similar to those in normal individuals .…”

Section: Candidate Biomarkers For Immune Monitoring In Allergen Immunmentioning

confidence: 99%

“…With the advances in multi‐parameter flow cytometry and the availability of many fluorochromes with high staining intensity, it has become possible to detect IgE‐expressing B cells following exclusion of naïve B cells that have bound IgE through surface CD23 as well as memory B cells expressing other Ig isotypes . This approach is technically demanding, and high cell numbers and an optimized flow cytometry panel are required for reliable detection . Similar to total and specific IgE in serum, blood IgE‐expressing B cells are increased in adults with atopic dermatitis, and in children with asthma, food allergy and atopic dermatitis .…”

Section: Candidate Biomarkers For Immune Monitoring In Allergen Immunmentioning

confidence: 99%

Recent developments and highlights in immune monitoring of allergen immunotherapy

Zelm

McKenzie

Varese

et al. 2019

Allergy

Self Cite

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Allergic diseases are the most common chronic immune-mediated disorders and can manifest with an enormous diversity in clinical severity and symptoms. Underlying mechanisms for the adverse immune response to allergens and its downregulation by treatment are still being revealed. As a result, there have been, and still are, major challenges in diagnosis, prediction of disease progression/evolution and treatment.Currently, the only corrective treatment available is allergen immunotherapy (AIT).AIT modifies the immune response through long-term repeated exposure to defined doses of allergen. However, as the treatment usually needs to be continued for several years to be effective, and can be accompanied by adverse reactions, many patients face difficulties completing their schedule. Long-term therapy also potentially incurs high costs. Therefore, there is a great need for objective markers to predict or to monitor individual patient's beneficial changes in immune response during therapy so that efficacy can be identified as early as possible. In this review, we specifically address recent technical developments that have generated new insights into allergic disease pathogenesis, and how these could potentially be translated into routine laboratory assays for disease monitoring during AIT that are relatively inexpensive, robust and scalable. K E Y W O R D Sallergen immunotherapy, allergy, biomarker, immune monitoring | 2343 ZELM Et aL.

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IgE‐expressing memory B cells and plasmablasts are increased in blood of children with asthma, food allergy, and atopic dermatitis

Cited by 62 publications

References 27 publications

Extracellular vesicles and asthma: A review of the literature

Extracellular vesicles and asthma: A review of the literature

Future research trends in understanding the mechanisms underlying allergic diseases for improved patient care

Recent developments and highlights in immune monitoring of allergen immunotherapy

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