IgE‐binding components of baker's yeast (Saccharomyces cerevisiae) recognized by immunoblotting analysis. Simultaneous IgE binding to mannan and 46–48 kD allergens of Saccharomyces cerevisiae and Candida albicans

Kortekangas-Savolainen, Outi; Kalimo, Kirsti; Lammintausta, Kaija; Savolainen, J.

doi:10.1111/j.1365-2222.1993.tb00879.x

Cited by 57 publications

(46 citation statements)

References 19 publications

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“…It has also been found in the cell wall fraction and in the culture supernatant of C. albicans [29, 30]. It has been identified as a highly conserved allergens [23]in many fungal species including S. cerevisiae [31, 32], C. albicans [32, 33, 34], C. herbarum [25]and A. alternata [23, 25]. It appears to be the most important protein allergen of S. cerevisiae and C. albicans [31, 32, 33, 34].…”

Section: Discussionmentioning

confidence: 99%

“…It has been identified as a highly conserved allergens [23]in many fungal species including S. cerevisiae [31, 32], C. albicans [32, 33, 34], C. herbarum [25]and A. alternata [23, 25]. It appears to be the most important protein allergen of S. cerevisiae and C. albicans [31, 32, 33, 34]. It was also recognized as an important allergen of C. herbarum and A. alternata [23, 35, 36].…”

Section: Discussionmentioning

confidence: 99%

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cDNA Cloning and Immunological Characterization of a Newly Identified Enolase Allergen from Penicillium citrinum and Aspergillus fumigatus

Lai¹,

Tam

Tang

et al. 2002

Int Arch Allergy Immunol

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Background:Penicillium citrinum and Aspergillus fumigatus are prevalent indoor airborne fungal species that have been implicated in human respiratory allergic disorders. It is important to understand the allergenic profile of these fungal species. The purpose of the present study is to characterize a newly identified enolase allergen from P. citrinum and A. fumigatus. Methods: Fungal proteins were separated by two-dimensional (2D) gel electrophoresis and blotted onto polyvinylidene difluoride membranes. Protein spots that reacted with IgE antibodies in serum samples from asthmatic patients were identified and the N-terminal amino acid sequences were determined by Edman degradation. The peptide sequences obtained were utilized in cloning the cDNA of the allergen genes by reverse transcriptase-polymerase chain reaction and the 5′- and 3′-rapid amplification cDNA end reactions. Results: Our results from 2D immunoblotting identified a 47-kD IgE-reactive component in the extracts of P. citrinum and A. fumigatus. The N-terminal amino acid sequences of the 47-kD proteins are homologous to those of fungal enolases. The corresponding enolase cDNA from P. citrinum contains 1,552 bp and encodes a protein of 438 residues. In A. fumigatus, the isolated enolase cDNA has 1,649 bp and contains a 438-amino acid open reading frame. The deduced amino acid sequences of these two enolases have 94% identity. These enolases from P. citrinum and A. fumigatus were expressed in Escherichia coli as a His-tagged protein and designated as rPen c 22 and rAsp f 22, respectively. Sera from 7 (30%) of the 23 Penicillium-sensitized asthmatic patients showed IgE binding to the 47-kD P. citrinum component (Pen c 22) and rPen c 22. In addition, six of seven Pen c 22-positive serum samples have IgE immunoblot reactivity to the 47-kD A. fumigatus component (Asp f 22) and rAsp f 22. A polyclonal rabbit antiserum generated against the N-terminal peptide of Pen c 22 can react with Pen c 22, rPen c 22, Asp f 22 and rAsp f 22. In addition, the presence of IgE cross-reactivity between rPen c 22 and rAsp f 22 and between enolases from A. fumigatus and Alternaria alternata was also detected by immunoblot inhibition. Conclusions: These results demonstrated that a novel enolase allergen from P. citrinum (Pen c 22) and A. fumigatus (Asp f 22) was identified. In addition, IgE cross-reactivity between enolase allergens from A. fumigatus and P. citrinum and between enolases from A. fumigatus and A. alternata was also detected. Results obtained provide more information on fungal enolase allergens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

cDNA Cloning and Immunological Characterization of a Newly Identified Enolase Allergen from Penicillium citrinum and Aspergillus fumigatus

Lai¹,

Tam

Tang

et al. 2002

Int Arch Allergy Immunol

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“…Kortekangas-Savolainen et al have studied the SPT results to an extract of S. cerevisiae in 226 patients with AD, 50 patients with allergic rhinitis and/or asthma, and 173 nonatopic controls (81). A positive SPT result was seen in 94% of patients with severe AD, 76% of patients with moderate AD, and 25% of patients with mild AD.…”

Section: Atopic Dermatitis and The Candida Yeastsmentioning

confidence: 99%

“…Kortekangas-Savolainen et al have tried to characterize the IgE binding components of S. cerevisiae (81). The found that the most frequent staining was found with a 48-kDa band.…”

Section: Atopic Dermatitis and The Candida Yeastsmentioning

confidence: 99%

Atopic Dermatitis and Fungi

Faergemann

2002

Clin Microbiol Rev

150

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Atopic dermatitis (AD) is a chronic, itching, inflammatory skin disease which is associated with asthma and/or hay fever and a familial occurrence of these conditions. Genetic factors are important in the development of AD, but the exact hereditary pathway is still unknown. Dry skin and the weakened barrier function in patients with AD is very important for the patient's reactions to irritants and other external trigger factors including microorganisms. The standard treatments are topical corticosteroids, topical immunomodulating agents, and emollients. If AD cannot be controlled by this type of treatment, systemic immunomodulating agents may be used. UVB, UVA, or psoralen-UVA may also be used for widespread severe lesions. However, some patients do not respond to these standard treatment, and then it is important to consider the role of microorganisms, house dust mites or food. The role of the Malassezia yeasts in AD, especially AD located to the head and neck region, is now documented in several papers. There are also several papers indicating the role of Candida as an aggravating factor in AD. Patients with AD also develop chronic dermatophyte infections more easily, and patients with AD and chronic dermatophyte infections may show improvement in their AD when treated with antifungal drugs

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“…Urticaria nicht bestätigen konnten oder eine Kreuzreaktivität zwischen Candida albicans und Pityrosporum ovale bzw. orbiculare sowie Saccharomyces cerevisiae zeigten [183,184,185,186]. Leider wurde in einer Studie von Middleton et al [134] …”

Section: Kann Eine Intestinale Candidabesiedelung Beim Menschen Zu Eiunclassified

Pathogenetische Bedeutung der intestinalen Candidabesiedelung

2004

Bundesgesundheitsbl - Gesundheitsforsch - Gesundheitsschutz

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IgE‐binding components of baker's yeast (Saccharomyces cerevisiae) recognized by immunoblotting analysis. Simultaneous IgE binding to mannan and 46–48 kD allergens of Saccharomyces cerevisiae and Candida albicans

Cited by 57 publications

References 19 publications

cDNA Cloning and Immunological Characterization of a Newly Identified Enolase Allergen from <i>Penicillium citrinum</i> and <i>Aspergillus fumigatus</i>

cDNA Cloning and Immunological Characterization of a Newly Identified Enolase Allergen from <i>Penicillium citrinum</i> and <i>Aspergillus fumigatus</i>

Atopic Dermatitis and Fungi

Pathogenetische Bedeutung der intestinalen Candidabesiedelung

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