IgE-binding and mast cell–activating capacity of the homologue of the major birch pollen allergen and profilin from Cannabis sativa

Ebo, Didier G.; Decuyper, Ine I.; Rihs, Hans‐Peter; Mertens, Christel; Gasse, Athina L. Van; Poorten, Marie-Line M. van der; Puysseleyr, Leander De; Faber, Margaretha A.; Hagendorens, Margo M.; Bridts, Chris H.; Sabato, Vito; Elst, Jessy

doi:10.1016/j.jaip.2021.02.012

Cited by 16 publications

(21 citation statements)

References 8 publications

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“…Recently, and as shown in Figure 4, we applied pMAT to identify Can s 2 (profilin) and Can s 5 (a Bet v 1 homologue) as new allergens of Cannabis sativa. 114 As indicated in the introductory paragraph, basophil and MC activation/degranulation can result from distinct IgEdependent and IgE-independent mechanisms. Therefore, it is likely that comparative analyses of dMAT and pMAT experiments, along with BATs, 109 might advance our understandings in the mechanisms that govern effector cell activation and degranulation.…”

Section: Passive Mast Cell Activation Tests (Pmats) In Ige-mediated Allergy: More Than a Diagnosticmentioning

confidence: 99%

Mast cell activation tests by flow cytometry: A new diagnostic asset?

Elst

Poorten

Gasse

et al. 2021

Clin Experimental Allergy

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Since the late nineties, evidence has accumulated that flow-assisted basophil activation test (BAT) might be an accessible and reliable method to explore the mechanisms governing basophil degranulation and diagnostic allowing correct prediction of the clinical outcome following exposure to the offending allergen(s) and cross-reactive structures for different IgE-dependent allergies and particular forms of autoimmune urticaria. Although the BAT offers many advantages over mediator release tests, it is left with some weaknesses that hinder a wider application. It is preferable to perform the BAT analysis within 4 h of collection, and the technique does not advance diagnosis in patients with non-responsive cells. Besides, the BAT is difficult to standardize mainly because of the difficulty to perform large batch analyses that might span over several days. This article reviews the status of flow cytometric mast cell activation test (MAT) using passively sensitized mast cells (MCs) with patients' sera or plasma (henceforth indicated as passive MAT; pMAT) using both MC lines and cultured MCs in the diagnosis of IgE-dependent allergies. In addition, this paper provides guidance for generating human MCs from peripheral blood CD34 + progenitor cells (PBCMCs) and correct interpretation of flow cytometric analyses of activated and/or degranulating cells. With the recent recognition of the mas-related G protein-coupled receptor X2 (MRGPRX2) occupation as a putative mechanism of immediate drug hypersensitivity reactions (IDHRs), we also speculate how direct activation of MCs (dMAT)-that

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Section: Passive Mast Cell Activation Tests (Pmats) In Ige-mediated Allergy: More Than a Diagnosticmentioning

confidence: 99%

Mast cell activation tests by flow cytometry: A new diagnostic asset?

Elst

Poorten

Gasse

et al. 2021

Clin Experimental Allergy

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“…24 Three new profilins received official designations, Pop n 2 from the black poplar (Populus nigra), 25 Amb t 8 from giant ragweed (Ambrosia trifida) pollen, 5 and Can s 2 from hemp (Cannabis sativa). 26 The profilin from sawtooth oak, Que ac 2, was also included in the ANDB. PR-10 proteins are conserved ligand-binding plant proteins.…”

Section: Plant-derivedmentioning

confidence: 99%

“…As profilins, ubiquitous cross‐reactive plant proteins, are significantly associated with allergy to grass pollen and to Cucurbitaceae fruits, 23 the clinical impact of profilin sensitizations is being continuously updated and expanded 24 . Three new profilins received official designations, Pop n 2 from the black poplar ( Populus nigra ), 25 Amb t 8 from giant ragweed ( Ambrosia trifida ) pollen, 5 and Can s 2 from hemp ( Cannabis sativa ) 26 . The profilin from sawtooth oak, Que ac 2, was also included in the ANDB.…”

Section: Respiratory Allergensmentioning

confidence: 99%

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Newly defined allergens in the WHO/IUIS Allergen Nomenclature Database during 01/2019‐03/2021

Sudharson

Kalic

Häfner

et al. 2021

Allergy

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Every scientific discipline relies on unambiguously naming and describing the molecules, cells, and organisms that are the objects of their studies. These are the tasks of the nomenclature committees that enable effective communication among the various research areas.For example, the International Union of Pure and Applied Chemistry 1 is the universally recognized authority on chemical nomenclature.The complexity of the immune system requires worldwide efforts on nomenclature issues. 2 These include consensus nomenclatures

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“…Negative sIgE values were excluded, as these cannot benefit from such an adjustment. Patients were selected from our previously published data 3,4 ; cannabis sativa allergic patients (CSA), controls with a pollen but no nsLTP sensitization (P+LTP‐), and controls with both pollen and nsLTP sensitizations (P+LTP+) were enrolled.…”

Section: Sige Hemp‐to‐tige Sige Rcan S 5‐to‐tige <002 ≥002 <0005 ≥000...mentioning

confidence: 99%

In search of the golden ratio for cannabis allergy: Utility of specific allergen‐to‐total IgE ratios

Decuyper

Rihs

Mertens

et al. 2021

Allergy

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LETTERS resulting from hypersensitivity. However, there is no feasible way to discern the type of immunologic pathway or causative agent. 2 If the cases are T-cell-mediated reactions against the SARS-CoV-2 spike glycoprotein, it is unclear if the magnitude of local reaction is related to systemic reaction in the context of infection. The morbilliform rash reported both as an adverse reaction of the BNT162b2 vaccine and as a manifestation of COVID-19 infection suggest the spike protein as a shared trigger of immune reactions. 7 The recurrence of a similar reaction after the second dose also needs to be carefully monitored. Among the mRNA-1273-related large local reactions, the recurrence rate was 50% and half of them were less severe than the initial reactions. 5 Considering the rarity and long time-to-onset (more than a week) interval of delayed local reaction, it might be regarded as irrelevant to vaccination or diagnosed as cellulitis, leading to unnecessary antibiotic treatment. This report might be the first to describe delayed local reaction resulting from ChAdOx1 nCoV-19 vaccination. Given the speed of mass vaccination, clinicians need to be aware of potential adverse reactions and prepared to provide appropriate management.

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IgE-binding and mast cell–activating capacity of the homologue of the major birch pollen allergen and profilin from Cannabis sativa

Cited by 16 publications

References 8 publications

Mast cell activation tests by flow cytometry: A new diagnostic asset?

Mast cell activation tests by flow cytometry: A new diagnostic asset?

Newly defined allergens in the WHO/IUIS Allergen Nomenclature Database during 01/2019‐03/2021

In search of the golden ratio for cannabis allergy: Utility of specific allergen‐to‐total IgE ratios

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