IgE antibodies to mountain cedar pollen predominantly recognize multiple conformational epitopes on Jun a 1

A single mouse monoclonal antibody, E58 modulates multiple IgE epitopes on group 1 cedar pollen allergens

“…Eleven adult subjects, who lived in the Austin, Texas area, where mountain cedar is a major part of the flora and produces a severe fall to wintertime allergic rhinitis, were recruited based on their diagnosis of mountain cedar allergy (cedar pollinosis) from Dr. van Bavel’s clinical research facility (Isis Clinical Research) (Goldblum et al , 2014). Ten and 14 subjects from France and Japan, where Italian cypress and Japanese cedar pollen cause similar symptoms, were recruited from Drs.…”

“…The reactivity of these antibodies to native and denatured Jun a 1 was analyzed by preincubating some of the Jun a 1 coated microtiter wells with 6 M Guanidine HCl for 30 min., before incubating both sets of wells with various concentration of E58 antibody. All wells were developed using HRP-anti mouse Ig (H&L) (Goldblum et al , 2014). …”

“…This makes Jun a 1 and its homologues excellent therapeutic targets. Our recently published data (Goldblum et al , 2014) suggests that human immune responses to Jun a 1 are predominantly (93–99%) directed against conformational epitopes.…”

“…Our laboratory has been exploring the molecular basis by which proteins from a relatively small number of protein families (pfams) induce allergic sensitization and subsequent reactions, using the dominant allergens from cedar pollens as models (Czerwinski et al , 2005; Goldblum et al , 2014; Ivanciuc et al , 2009). We identified and characterized two major allergens from the mountain cedar pollen (Midoro-Horiuti et al , 1999; Midoro-Horiuti et al , 2000), which dominate the landscape of the South Central US and are responsible for fall and winter pollinosis, commonly called “cedar fever.” Similar allergens were recognized for Japanese cedar and subsequently cypress trees in southern Europe.…”

“…We identified and characterized two major allergens from the mountain cedar pollen (Midoro-Horiuti et al , 1999; Midoro-Horiuti et al , 2000), which dominate the landscape of the South Central US and are responsible for fall and winter pollinosis, commonly called “cedar fever.” Similar allergens were recognized for Japanese cedar and subsequently cypress trees in southern Europe. Recent clinical studies from our lab, indicate that IgE antibodies against the single allergen Jun a 1, dominates (average of 93% of IgE reactivity) the allergic IgE antibody response to mountain cedar pollen (Goldblum et al , 2014). This makes Jun a 1 and its homologues excellent therapeutic targets.…”