IFNγ/TNFα specific-cells and effector memory phenotype associate with active tuberculosis

Beyond the IFN- horizon: biomarkers for immunodiagnosis of infection with Mycobacterium tuberculosis

“…Several recent reports suggest that LTBI and infection control are dominated by central memory T-cells with potential of IL-2 and optionally IFN-c co-secretion; whereas active tuberculosis is characterised by loss of IL-2 production and T-cells with effector memory T-cell phenotype [101,104,139,141,[146][147][148]. Along these lines, HARARI et al [149] evaluated CD4 T-cells producing TNF-a, IFN-c and IL-2 by flow cytometry in subjects with active tuberculosis or LTBI.…”

Measurement of Phenotype and Absolute Number of Circulating Heparin-Binding Hemagglutinin, ESAT-6 and CFP-10, and Purified Protein Derivative Antigen-Specific CD4 T Cells Can Discriminate Active from Latent Tuberculosis Infection

“…tuberculosis antigen-specific CD4 T cells between active and latent TB populations but saw a significant increase in the TNF-␣ ϩ IFN-␥ ϩ IL-2 Ϫ frequency in active TB patents (8). Another study that measured a range of cytokines found no differences at all (16).…”

“…Another approach has been to measure cell surface proteins associated with particular states of the memory response on M. tuberculosis antigen-specific CD4 T cells. Markers that have been suggested to differentiate the two groups include CD27 (6, 7), CD45RA and CCR7 (8), and CD127 (9). Finally, another possible avenue of discrimination has been the identification of particular M. tuberculosis antigens that appear to have stronger T cell responses in the latent population (10).…”

Functional and Phenotypic Analysis of Two-Day Monocyte-Derived Dendritic Cells Suitable for Immunotherapy Purposes

“…For Th1 T cell differentiation, we used Mtb. peptides previously described to induce IFN-γ secretion in immune TB donors [26,57,58]. In the present study, we compared the capacity of peptidepulsed DCs to induce a Th1 phenotype with that of latent-infected donor cells cultured with purified CD4 T cells.…”