IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis

Abstract

Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients.Objective:To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS).Material and Methods:Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-γ R (IFN-γR1 and IFN-γR2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-γ was measured by the ELISA technique.Results:No significant differences were found in the IFN-γR1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-γR2 expression it was found that IFN-γR2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-γ concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-γ levels and the expression of IFN-γR1 and IFN-γR2 was found. However, a positive correlation between IFN-γ levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found.Conclusion:The study of IFN-γR expression in gingival tissue samples from patients with CP showed an increase only in the IFN-γR2 chain in endothelial cells when compared to HS.

Inverse behavior of IL-23R and IL-17RA in chronic and aggressive periodontitis

“…Clinical parameters (PD, CAL, and %BoP) were measures at 6 sites per tooth, and periapical radiographs were obtained by the same researcher using the long-cone paralleling technique with the same radiographic equipment, film, exposure, and development conditions for all subjects to obtain the diagnosis [ 1 30 31 ].…”

“…This group included 16 patients (15 women and 1 man; median age, 40 years; interquartile range [IQR], 12 years) who attended the clinic for dental esthetic surgery (crown lengthening procedures) without clinical inflammation, or BoP, with ≤3 mm PD, and with no evidence of CAL, or radiographic bone loss [ 1 30 31 ].…”

“…This study group included 23 patients (19 and 4 men; median age, 46 years; IQR, 21 years) who presented pockets in more than 30% of the oral cavity, including ≥5 mm CAL and ≥6 mm PD with radiographic evidence of alveolar bone loss [ 1 30 31 ].…”

“…This group comprised 14 GAP patients (10 women and 4 men; median range, 32 years; IQR, 5.75 years) who had a family history of ≥1 family member with a history of severe periodontal problems. Showing CAL in more than 3 teeth further than the incisors and first molars, with a minimum of ≥5 mm CAL, ≥6 mm PD and radiographic evidence of advanced alveolar bone loss [ 1 30 31 ].…”

ILC1s and ILC3s Exhibit Inflammatory Phenotype in Periodontal Ligament of Periodontitis Patients

“…In addition, IL-17A can synergize with other inflammatory cytokines to induce inflammatory responses, which result in destruction of periodontal tissues and alveolar bone (34). Similar to IL-17A, IFN-g is also increased in periodontitis patients (35). IFN-g has been shown not only to enhance the inflammation through induction of chemokines production by epithelial cells (36) but also to promote the destruction of periodontal tissues by inducing T cells to produce TNF-a and RANKL (37).…”