Idiopathic macular hole: history and status quo review

Samoylov, A.N.; Khaibrakhmanov, T.R.; Fazleeva, G A; Samoylova, P A

doi:10.17116/oftalma20171336131-137

Cited by 8 publications

(5 citation statements)

References 46 publications

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“…FTMH is a vital cause of central visual loss worldwide, with an estimated incidence of 7.8 persons/100,000 population per year [5]. Treatment strategies for FTMH previously consisting of relieved tractions by the thorough removal of the vitreous humor with gas tamponade (first described by Kelly and Wendel), now consist of ILM peeling and removal of proliferations at the retinal surface combined with indocyanine green injection [6, 28].…”

Section: Discussionmentioning

confidence: 99%

Nerve growth factor promotes the proliferation of Müller cells co-cultured with internal limiting membrane by regulating cell cycle via Trk-A/PI3K/Akt pathway

Zhang

Lin

et al. 2019

BMC Ophthalmol

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Background Nerve growth factor (NGF), produced by Müller cells, and internal limiting membrane (ILM) have fundamental roles in the development of full-thickness macular hole (FTMH). However, the potential crosstalk between NGF and ILM in FTMH is unclear. This study aimed to explore the mechanism and effects of NGF on the proliferation of Müller cells co-cultured with ILM. Methods Primary Müller cells and ILM from New Zealand rabbits were extracted and authenticated with specific staining. Müller cells co-cultured with or without ILM were exposed to NGF and then analysed. Müller cell viability was estimated using cell counting kit-8. Cell cycle analysis was performed by flow cytometry. The levels of cell cycle-related gene were detected using qRT-PCR. The TrK-A/Akt signal axis and downstream signaling cascades such as p21, CyclinE, CDK2, CyclinD1, and CDK4 were investigated by western blotting. Results ILM treatment alone induced the proliferation of Müller cells following the promotion of phosphorylated Akt, while growth of Müller cells was enhanced by activation of the Trk-A/Akt pathway under the stimulation of NGF or NGF + ILM. Additionally, the ratio of S-phase cells was increased, while G2-phase cells decreased upon the treatment with either ILM or NGF alone, or with NGF + ILM co-treatment. Cell cycle-related genes such as CyclinD1, CyclinE, CDK2, and CDK4 were all upregulated, but p21 expression was downregulated in the presence of NGF, ILM, or NGF + ILM. There was an additive effect on cell proliferation and cell cycle in the group of Müller cells exposed to NGF co-cultured with ILM compared with either NGF or ILM treatment alone. However, both K252ɑ (inhibitors of Trk-A) and LY294002 (inhibitor for Akt) counteracted the effect of NGF or NGF + ILM on the protein levels of Trk-A, Akt, CyclinD1, CyclinE, CDK2, and p21. Conclusions Müller cells co-cultured with ILM or NGF promoted cell proliferation by regulating cell cycle-correlated proteins via the PI3K/Akt pathway. ILM + NGF further amplified the PI3K/Akt signaling pathway by binding to Trk-A, leading to more cell growth. This study provides new insight into the potential mechanism of NGF-mediated proliferation of Müller cells co-cultured with or without ILM, which may have considerable impact on therapies for FTMH. Electronic supplementary material The online version of this article (10.1186/s12886-019-1142-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Nerve growth factor promotes the proliferation of Müller cells co-cultured with internal limiting membrane by regulating cell cycle via Trk-A/PI3K/Akt pathway

Zhang

Lin

et al. 2019

BMC Ophthalmol

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“…At the same time (according to the classification), in all the cases this was a primary MH without vitreomacular traction [33,40].…”

Section: Clinical Study 41 Methods and Results Of The Application Of ...mentioning

confidence: 86%

“…They are biologically inert substances used to stain intraocular structures in different vitreoretinal pathologies. In order to choose the optimum vital dye, we carried out experimental staining of VRI by suspensions «Kenalog-40» and «Vitreocontrast», which made it possible to assess their main characteristics and to choose intraoperative staining of the eye posterior segment, to evaluate the results of the comparative staining by suspensions Kenalog-40 and Vitreocontrast in the PVD induction during the experimental study [27,[31][32][33].…”

Section: Experimental Studymentioning

confidence: 99%

Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis and the Classification of Idiopathic Macular Holes

Kislitsyna¹,

Novikov²

2023

Medical and Surgical Retina - Recent Innovation, New Perspective, and Applications

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Application of the developed technology of vitreous body visualization in patients with macular holes (MH) of different sizes provides a principally new approach to clinical and diagnostic examination based on the development (depending on the stage of the pathological process) of basic classification anatomical and morphological signs (elongation of the vitreous cisterns in anteroposterior direction, violation of the integrity of the vitreous cistern wall and probability of staining composition exit beyond stained cavities, the degree of adhesion of the vitreous body on the internal limiting surface, etc). and characterized by advantages over traditional MH classifications, which in general (based on the outlined recommendations) makes it possible to significantly increase the clinical effectiveness of vitreoretinal surgical intervention in comparison with traditional classifications.

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“…При ИМР и рецидивах, если ранее не была удалена ВПМ, применяется повторная витрэктомия и техника перевернутого лоскута с модификациями, а также метод с использованием богатой тромбоцитами плазмы крови (БОТП) как наиболее простой и оптимальный для достижения повторного закрытия разрыва [3]. Появление оптической когерентной томографии (ОКТ) и развитие витреоретинальной хирургии значительно расширило возможности диагностики и лечения различной патологии сетчатки, включая макулярные разрывы [4]. Отмечены положительные результаты с улучшением остроты зрения у пациентов после использования методики закрытия ИМР с применением БОТП [5].…”

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Сlinical Case of Macular Hole Recurrence after Surgical Treatment with Platelet-Rich Plasma

Черепов¹,

Гозиев²

2020

Oftalʹmologiâ

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Purpose: presentation of a clinical case of an idiopathic macular hole (IMH) recurrence after its successful closure augmented with platelet-rich plasma (PRP) and the results of repeated surgical treatment.Methods. The patient underwent clinical examination and optical coherence tomography (OCT) of the retina before and after surgical treatment. А 2-stage surgical treatment of the left eye with an interval of 7 days: phacoemulsification (PE) followed by microinvasive vitrectomy with Internal limiting membrane peeling (ILM) and closure with the use of PRP. The patient was discharged with improved visual acuity and closure of the macular hole (MH). After 2 weeks vision deterioration was noted by the patient in the operated eye. Clinical examination revealed macular edema (ME) and recurrence of MH. Topical steroid eye drops were used to no avail, with increase of ME and MH via OCT control. Revision of the vitreal cavity augmented with PRP was performed.Results. In follow-up a complete closure of the MH, resolution of ME and improvement in visual acuity after repeated surgery was confirmed.Conclusions. A case of recurrent MH after primary closure with PRP is described. The case suggests a possibility of clinical improvement after repeated MH closure augmented with PRP. The causes and mechanisms of the recurrence of MH are still not clear. Despite the positive results of surgery subsequent OCT retina examination is necessary.

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Idiopathic macular hole: history and status quo review

Abstract: The article reviews the literature on one of the topical problems of vitreoretinal surgery - idiopathic macular holes. The history, concept, classification and diagnostics, as well as surgical and alternative treatment methods of macular holes are explored.

Cited by 8 publications

References 46 publications

Nerve growth factor promotes the proliferation of Müller cells co-cultured with internal limiting membrane by regulating cell cycle via Trk-A/PI3K/Akt pathway

Nerve growth factor promotes the proliferation of Müller cells co-cultured with internal limiting membrane by regulating cell cycle via Trk-A/PI3K/Akt pathway

Perspective Chapter: The Vitreous Body Visualization Technique in Diagnosis and the Classification of Idiopathic Macular Holes

Сlinical Case of Macular Hole Recurrence after Surgical Treatment with Platelet-Rich Plasma

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