Identity of molecules bearing murine ia alloantigenic specificities Ia.7 and Ia.22: Evidence for a single type of I-E molecule in homozygotes

Cullen, Susan E.; Shreffler, Donald C.; Kindle, Cathy S.; David, Chella S.

doi:10.1007/bf01567823

Cited by 13 publications

(4 citation statements)

References 23 publications

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“…The different glycosylation of the Q1O gene product by L cells and liver is not surprising as other proteins are known to be glycosylated differently in different cell types, e.g., class II molecules (Cullen et al, 1981). The serum form and the L cell secreted Q1O gene product are identical in size after endo F treatment.…”

Section: Discussionmentioning

confidence: 99%

Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.

Devlin¹,

Lew²,

Flavell³

et al. 1985

The EMBO Journal

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The DNA sequence of the Q10 genes appears to be highly conserved amongst strains of mice and has only been found to be transcribed in the liver. An examination of the nucleotide sequence of the exon that normally encodes the transmembrane domain of class I molecules suggested that the Q10 gene encodes a secreted protein. We have established this by showing that L cells transformed with an expression vector containing the Q10 gene secrete a class I molecule which was identified with an antiserum raised against a peptide predicted by the Q10 transmembrane exon. Both the L cell‐derived Q10 molecule and a class I protein immunoprecipitated from serum with this anti‐peptide antiserum have mol. wts. of approximately 38 000; the Q10 molecule secreted by L cells is heterogeneous in mol. wt. This heterogeneity was drastically reduced after endoglycosidase F treatment, suggesting that Q10 molecules secreted into the serum by the liver may be glycosylated differently from those secreted by L cells. Endoglycosidase F treatment of both the L cell and serum forms of the soluble molecule yielded two products with mol. wts. of approximately 32 000 and 35 000; this is consistent with the observation that the predicted Q10 protein sequence has two potential glycosylation sites. In contrast to previous published results, the Q10 molecule reacted with rabbit anti‐H‐2 antisera which is consistent with its greater than 80% homology to the classical transplantation antigens.

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Section: Discussionmentioning

confidence: 99%

Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.

Devlin¹,

Lew²,

Flavell³

et al. 1985

The EMBO Journal

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show abstract

“…The above evidence is not conclusive, but nevertheless is suggestive of the homology between DC and 1-A. The possibility that in the mouse a third subset of Ia molecules exists is controversial; earlier reports suggested that I-E molecules can be subdivided into two subsets (Delovitch & Barber 1979), while a more recent report denies such a possibility (Cullen et al 1980).…”

Section: Dr Locus Br Locus DC Locusmentioning

confidence: 93%

The Genetic Control of Human Ia Alloantigens: A Three‐Loci Model Derived from the Immunochemical Analysis of ‘Supertypic’ Specificities

Tanigaki

Tosi

1982

Immunological Reviews

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“…Ia specificity 2, which was acquired by schistosomula recovered from C3H/Crgl donors, has been previously shown to map to the I-A subregion (David 1976). Specificity 7, also found on the tegumental surface of C3H/Crgl derived larvae, maps to the I-E subregion (David & Cullen 1978, Cullen et al 1980. Alloantiserum identifying the Ia.8 specificity, which is under I-A control (David 1976), reacted strongly with worms recovered from the lungs of B10 mice.…”

Section: Acquisition Of K a N D D Gene Productsmentioning

confidence: 99%

Murine alloantigen acquisition by schistosomula of Schistosoma mansoni: Further evidence for the presence of K, D, and I region gene products on the tegumental surface

Gitter

Damian

1982

Parasite Immunology

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Previous studies have demonstrated that shistosomula, recovered from the lungs of Schistosoma mansoni-infected mice, acquire H-2Kk and both Ik and Is gene products. We confirmed and extended those observations by identifying several individual antigenic specificities mapping not only to H-2Kk and I-Ak (Sher, Hall & Vadas 1978), but to H-2Dk, I-Ek, H-2Kb, H-2Db and I-Ab as well. Private H-2 specificities 23 (H-2Kk) and 32 (H-2Dk) were identified on the tegumental surface of schistosomula isolated from C3H/Crgl mice (H-2k). Larvae isolated from C57Bl/10Sn (B10) mice (H-2b) were shown to acquire private specificities 33 and 2, which map to H-2Kb and H-2Db respectively. I region associated (Ia) antigens coded for by genes mapping in the I-A subregion (Ia.2 and 3) and I-E subregion (Ia.7) were also acquired from C3H/Crgl mice. Schistosomula from B10 donors were shown to acquire Ia specificities 3 and 8, which are under I-A subregion control. Evidence that monoclonal antibodies recognize H-2 antigens on the larval surface is also demonstrated.

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Identity of molecules bearing murine ia alloantigenic specificities Ia.7 and Ia.22: Evidence for a single type of I-E molecule in homozygotes

Cited by 13 publications

References 23 publications

Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.

Secretion of a soluble class I molecule encoded by the Q10 gene of the C57BL/10 mouse.

The Genetic Control of Human Ia Alloantigens: A Three‐Loci Model Derived from the Immunochemical Analysis of ‘Supertypic’ Specificities

Murine alloantigen acquisition by schistosomula of Schistosoma mansoni: Further evidence for the presence of K, D, and I region gene products on the tegumental surface

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