Identity of hemolysins produced by Bacillus thuringiensis and Bacillus cereus

Honda, T.

doi:10.1016/0378-1097(91)90087-q

“…The monoclonal anti-thuringolysin antibody was provided by Professor Takeshi Honda (Research Institute for Microbial Diseases, Osaka University, Japan) who described the identity of hemolysin of Bt and B. cereus [13]. Ready to use preparation at 50 Wg Wl 31 was stored at +4³C.…”

Section: Monoclonal Anti-thuringolysin Antibodymentioning

confidence: 99%

Super-infection by Bacillus thuringiensis H34 or 3a3b can lead to death in mice infected with the influenza A virus

Hernandez

¹

2000

FEMS Immunology and Medical Microbiology

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Bacterial super-infections are the main cause of complication and mortality after influenza virus (IAV) infection. Since Bacillus thuringiensis (Bt) is considered non-pathogenic for humans and is widely sprayed in urban areas, the aim of this work was to evaluate the potential pathogenicity of a combined infection Bt-IAV in a mouse model of pneumonia. Bacteria used for super-infections were Bt serotype H34 isolated from human infection and the insecticidal strain 3a3b obtained from a commercial source. Virus strain was A/Scotland/20/74 (H3N2) adapted to BALB/c mice by serial lung passage. Combined infection with 4% of the viral lethal dose 50% (LD(50)) and 10(2) spores of Bt H34 killed 40% of the mice. Mortality rates increased up to 55% and 100% when combined infections were done with respectively 10(4) and 10(7) spores. The insecticidal strain Bt 3a3b was less pathogenic than Bt H34. A dose of 10(4) spores associated with 4% of IAV LD(50) killed 50% of the mice. This inoculum must be compared with the doses usually sprayed in agriculture: 10(11) spores m(-2). Total protection against super-infection was obtained when mice were treated with amantadine. Even if only a few cases of Bt human infection have been reported, these results suggest a possible risk for workers spraying Bt-based biopesticides during flu outbreaks.

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“…Since the obvious lesions were lung haemorrhagic suffusions, we supposed, as previously described in the mice cutaneous infectious model [1], that the secreted haemolysins of B. thuringiensis were the toxins involved in the pathogenicity. Two different haemolysins, closely related to those of Bacillus cereus , have been described [5–7]. While one, thiol‐dependent, is inhibited by cholesterol, the other is not.…”

Section: Resultsmentioning

confidence: 99%

Bacillus thuringiensisserotype H34 isolated from human and insecticidal strains serotypes 3a3b and H14 can lead to death of immunocompetent mice after pulmonary infection

Hernandez

¹

,

Ramisse²,

Cruel

³

et al. 1999

FEMS Immunology &Amp; Medical Microbiology

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In 1995, we isolated a strain of Bacillus thuringiensis serotype H34 from severe human tissue necrosis. This bacterium was able to induce myonecrosis in immunosuppressed mice after cutaneous infection. Its potential pathogenicity for immunocompetent hosts was investigated in a mouse model of pulmonary infection. Mice infected intranasally by a suspension containing 10(8) spores died within 8 h in a clinical toxic-shock syndrome. In the same conditions, infection with a mutant without crystalline toxin, with the supernatant from a culture containing 10(8) bacteria ml(-1) and by the insecticidal strain serotypes 3a3b or H14 led to identical results. Lower inocula simply induced a local inflammatory reaction with bacterial persistence observed during the course of 10 days.

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“…The monoclonal anti‐thuringolysin antibody was provided by Professor Takeshi Honda (Research Institute for Microbial Diseases, Osaka University, Japan) who described the identity of hemolysin of Bt and B. cereus [13]. Ready to use preparation at 50 μg μl −1 was stored at +4°C.…”

Section: Methodsmentioning

confidence: 99%

Super-infection byBacillus thuringiensisH34 or 3a3b can lead to death in mice infected with the influenza A virus

Hernandez

¹

,

Ramisse²,

Gros

³

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

View full text Add to dashboard Cite

Bacterial super-infections are the main cause of complication and mortality after influenza virus (IAV) infection. Since Bacillus thuringiensis (Bt) is considered non-pathogenic for humans and is widely sprayed in urban areas, the aim of this work was to evaluate the potential pathogenicity of a combined infection Bt-IAV in a mouse model of pneumonia. Bacteria used for super-infections were Bt serotype H34 isolated from human infection and the insecticidal strain 3a3b obtained from a commercial source. Virus strain was A/Scotland/20/74 (H3N2) adapted to BALB/c mice by serial lung passage. Combined infection with 4% of the viral lethal dose 50% (LD(50)) and 10(2) spores of Bt H34 killed 40% of the mice. Mortality rates increased up to 55% and 100% when combined infections were done with respectively 10(4) and 10(7) spores. The insecticidal strain Bt 3a3b was less pathogenic than Bt H34. A dose of 10(4) spores associated with 4% of IAV LD(50) killed 50% of the mice. This inoculum must be compared with the doses usually sprayed in agriculture: 10(11) spores m(-2). Total protection against super-infection was obtained when mice were treated with amantadine. Even if only a few cases of Bt human infection have been reported, these results suggest a possible risk for workers spraying Bt-based biopesticides during flu outbreaks.

show abstract

Identity of hemolysins produced by Bacillus thuringiensis and Bacillus cereus

Cited by 15 publications

References 1 publication

Super-infection by Bacillus thuringiensis H34 or 3a3b can lead to death in mice infected with the influenza A virus

Super-infection by Bacillus thuringiensis H34 or 3a3b can lead to death in mice infected with the influenza A virus

Bacillus thuringiensisserotype H34 isolated from human and insecticidal strains serotypes 3a3b and H14 can lead to death of immunocompetent mice after pulmonary infection

Super-infection byBacillus thuringiensisH34 or 3a3b can lead to death in mice infected with the influenza A virus

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