Identity, Diversity, and Molecular Phylogeny of the Endophytic Mycobiota in the Roots of Rare Wild Rice (
            <i>Oryza granulate</i>
            ) from a Nature Reserve in Yunnan, China

Youxiang, Zhuang; Zhang, Chulong; Lin, Fu‐Cheng; Kubicek, Christian P.

doi:10.1128/aem.01911-09

Cited by 120 publications

(64 citation statements)

References 64 publications

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“…The melanised hyphae, typical for DSE fungi, are considered to be of importance for the host to survive stress conditions because cell wall melanin can trap and eliminate oxygen radicals generated during abiotic stress (Yuan et al 2010). This gives a clear idea about the abiotic stress tolerance exhibited by the seagrass are accompanied by the role of endophytic fungi by trapping the free oxygen radicals.…”

Section: Discussionmentioning

confidence: 99%

Differential methods of localisation of fungal endophytes in the seagrasses

2016

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Sections of three seagrass species (Halophila ovalis, Cymodocea serrulata and Halodule pinifolia) were assessed for endophytes based on differential staining using light and fluorescence microscopy method. Acridine orange and aniline blue detected endophytic fungi in 20% and 10% of the segments, respectively, whereas lactophenol cotton blue was more sensitive to detect the fungal hyphae in 70% of the segments. Hyphae were the principal fungal structures generally observed under the cuticle, within the epidermal cells, mesophyll (Parenchyma) cells and occasionally within the vascular tissue that varied in type, size and location within the leaf tissue. Present study also recorded the sporulation for the first time from the seagrass endophytes. Successfully amplified products of the ITS region of endophytic fungal DNA, directly from seagrass tissue and also from culture-dependent fungal DNA clearly depicted the presence of endophytic fungi in H. ovalis with two banding patterns (903 and 1381 bp) confirming the presence of two dominant fungal genera. The fingerprinting of endophytic fungal community within the seagrass tissue was assessed using denaturing gradient gel electrophoresis (DGGE) that derived with multiple bands that clarified the presence of more than one taxon within the seagrass tissue.

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Section: Discussionmentioning

confidence: 99%

Differential methods of localisation of fungal endophytes in the seagrasses

2016

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“…Roots (Yuan et al, 2009) and stems (Yuan et al, 2010) have been used for the isolation of endophytes in rice plants. In the present study, seeds, roots, leaves and stems of both rice varieties were used and endophytes were isolated from all the parts used.…”

Section: Discussionmentioning

confidence: 99%

“…Due to their capability of colonizing internal tissues of plants and their ability to promote plant growth and to control plant diseases, endophytic microorganisms can be used in agriculture as a tool to improve crop performance (Yuan et al, 2009). In the past two decades, a great deal of information on the diversity and the role of endophytes have been collected (Yuan et al, 2009). …”

Section: June 2015mentioning

confidence: 99%

Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties

Atugala¹,

Deshappriya²

2015

J. Natn. Sci. Foundation Sri Lanka

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Traditional rice varieties, although low producers of yield, are more amenable to organic farming practices that cause less damage to the environment and human health. Improved growth and reduced disease incidence would produce higher yields, and endophytic fungi have proven to be effective in achieving these ends in wheat. With this in view, endophytic fungal assemblages of two traditional rice varieties of Sri Lanka i.e. Suwandel and Kaluheenati were evaluated for their effect on the growth and disease incidence of rice plants, because the endophytic mycoflora of these two traditional rice varieties and their effects have not been studied before. The most common and the highest occurring endophytes of both rice varieties i.e. Absidia and Cylindrocladium were tested to determine their effect on rice plant growth and the results showed that the plants inoculated with both fungal isolates showed significant increases (p ≤ 0.05) in plant height, fresh weight and dry weight. Twenty two endophytic fungal isolates common to both rice varieties were screened using dual culture assay for their ability to control the mycelial growth of Magnaporthe grisea, the causative agent of rice blast disease. All tested endophytes controlled pathogen growth by coiling the hyphae around the pathogen and forming clamps and loops. However, Absidia and Acremonium showed the highest growth inhibition of the pathogen (100 %) and showed an abundance of the above inhibitory structures. The effect of crude culture filtrates of thirteen endophytic fungi tested using the diffusion plate method indicated antagonistic activity against the rice pathogen. In planta tests were carried out to assess the effect of three endophytic fungi i.e. Acremonium, Absidia and Penicillium on infection by M. grisea. Disease symptoms were observed only on the leaves of the rice plants grown from Penicillium inoculated seedlings and on plants used as controls sprayed with a 1×10 7 spores/mL suspension of the pathogen. Pre-inoculation with Acremonium and Absidia were effective in preventing infection by the blast fungus. No disease symptoms were observed in plants pre-inoculated with endophytes and in plants used as controls sprayed with a 1×10 5 spores/mL suspension of the pathogen. Twenty one endophytic fungi common to both rice varieties were assessed by the culture plate method in this study.

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“…Roots were cut into 7 cm long pieces and washed under running tap water for 20 minutes. Squashed preparations of root segments were observed under the light microscope and the presence of root inhabiting endophytic fungi was confirmed by trypan blue staining method (Yuan et al 2009). …”

Section: Materials and Methods Sample Collection And Microscopic Obsementioning

confidence: 99%

“…Diazotrophic bacterial and actinomycete populations of rice are reported to promote plant growth promotion and to possess the potential for nitrogen fixation and disease resistance (Barraquio et al 1997, Tian et al 2004. Fungal endophytes also have been detected in cultivated rice and they have shown to possess antagonistic or plant growth stimulating properties (Yuan et al 2009, Naik et al 2009). Previous studies in Sri Lanka have shown that some endophytes present in the traditional rice varieties Suwandel, Kaluheenati and Herath Banda have the ability to increase the growth of plants significantly (Atugala & Deshappriya 2015, Ponnawila & Deshappriya 2014.…”

Section: Introductionmentioning

confidence: 99%

An inoculum of endophytic fungi for improved growth of a traditional ricevariety in Sri Lanka

Wijesooriya¹,

Deshappriya²

2016

Trop. Plant Res.

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Use of chemical fertilizers and pesticides in rice cultivation has incurred many environmental and health problems in Sri Lanka. Therefore, there is renewed interest in cultivating traditional rice varieties as they are more amenable to organic farming practices. However, as the yield of these varieties is comparatively low, strategies to enhance their performance should be investigated. As endophytes of plants are reported to promote growth and yield of a number of crop varieties, this study was aimed at studying the endophytic fungal assemblage present in the traditional rice variety Kuruluthuda with a view to evaluate their capacity to enhance plant growth and yield. Twenty seven endophytic fungal species were isolated from different parts of Kuruluthuda rice plants collected from a paddy field cultivated using organic fertilizers in the Gampaha district, Sri Lanka. Two frequently isolated endophytic fungal spp. i.e. Acremonium and Arthrobotrys (frequencies of isolation 60% and 38.6% respectively) were introduced separately and in combination torice seedlings using spore suspension and plate methods to determine their effect on growth and yield under green house and field conditions. All endophyte inoculated plants showed a significant difference (P ≤ 0.05) in plant growth (height, fresh weight and dry weight), number of tillers and yield when compared withnon-inoculated plant sunder both green house and field conditions. The effect of Acremonium and Arthrobotrys when introduced in combination showed a significant difference (P ≤ 0.05) in the fresh weight, dry weight, tiller number and yield (weight of seeds harvested) when compared to their individual effects under field conditions which indicates that the two endophytes in combination can be used as a better inoculum to improve biomass and yield of the plants of rice variety tested. This is the first report of the endophytic mycoflora of the rice variety Kuruluthuda and their potential use for growth promotion and yield enhancement.

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Identity, Diversity, and Molecular Phylogeny of the Endophytic Mycobiota in the Roots of Rare Wild Rice ( Oryza granulate ) from a Nature Reserve in Yunnan, China

Cited by 120 publications

References 64 publications

Differential methods of localisation of fungal endophytes in the seagrasses

Differential methods of localisation of fungal endophytes in the seagrasses

Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties

An inoculum of endophytic fungi for improved growth of a traditional ricevariety in Sri Lanka

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