Identity and Prevalence of Mosaic-inducing Cucurbit Viruses in Shiraz, Iran)

Rahimian, Heshmatollah; Izadpanah, K.

doi:10.1111/j.1439-0434.1978.tb03620.x

Cited by 13 publications

(7 citation statements)

References 12 publications

(7 reference statements)

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“…Production of chlorotic local lesions on leaves of inoculated C. amaranticolor agrees with earlier investigations with WMV [16,20]. It was also reported that some WMV isolates produce local lesions on C. quinoa, some produce systemic symptoms, whereas others do not infect it at all [20,23]. This may probably help to explain the lack of infection of this plant species with the virus isolate dealt with in this study.…”

Section: Host Range Of the Virussupporting

confidence: 90%

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Santosa¹,

Al-Shahwan²,

Abdalla³

et al. 2018

JAST-A

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In one of the field visits to cucurbits-growing areas in vicinity of Riyadh city during 2013-2015, severe virus disease-like symptoms were observed on watermelon in Al-Ammariyah area. Mechanical inoculation of the different plant species used in the host range study, from the collected symptomatic watermelon samples, produced mosaic symptoms on Citrullus lanatus, Cucumis sativus, Cucurbita pepo, C. melo, C. melo subsp. melo and Nicotiana benthamiana, but chlorotic local lesions on Chenopodium amaranticolor. No symptoms were observed on the rest of the other inoculated plant species. The virus was transmitted by Aphis gossypii and A. craccivora in a non-persistent manner. Transmission electron microscopic examination of watermelon samples using the leaf dip method revealed only microscopic filamentous shaped virus particles measuring 750 nm in length and 12 nm in diameter in average. ELISA revealed positive results only to Watermelon mosaic virus (WMV) and negative to Zucchini yellow mosaic virus (ZYMV), Papaya ringspot virus (PRSV), Cucumber mosaic virus (CMV) and Squash mosaic virus (SqMV). Specific bands of approximately 825 bp were formed on agarose gel following electrophoresis of the reverse transcriptase-polymerase chain reaction (RT-PCR) products of each of the naturally infected C. lanatus, and artificially infected C. lanatus, C. pepo, C. sativus, and N. benthamiana. The homology tree that was constructed from multiple sequence alignments of the detected Saudi Arabian isolate of WMV (WMV-SA) with 18 other isolates of WMV from nine different countries indicated close relationships between them. Two isolates from Spain and two other isolates from Iran were more closely related to the WMV-SA whereas the isolate from Poland was the least.

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Section: Host Range Of the Virussupporting

confidence: 90%

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Santosa¹,

Al-Shahwan²,

Abdalla³

et al. 2018

JAST-A

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“…WMV is the most prevalent potyvirus infecting cucurbits in Iran [16,21] and other countries [10,14]. WMV has a [20].…”

Section: Discussionmentioning

confidence: 99%

“…Although WMV has long been known to occur in several regions of Iran [9,16,19,21], a 218-nucleotide sequence of the CP N-terminal region of eight isolates from Iran has just recently been reported [8]. The goals of the present study were amplification of CP gene and assessment of the genetic variation among the CP sequences of the representative isolates collected from several regions of Iran.…”

Section: Introductionmentioning

confidence: 98%

Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran

et al. 2008

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Watermelon mosaic virus (WMV) is one of the most important viruses that causes different symptoms in Cucurbitaceae. WMV is a potyvirus with a worldwide distribution, but occurs most commonly in temperate and Mediterranean regions. Cucurbit species grown in Yazd, Esfahan, West Azerbaijan, Hormozgan, and Kerman provinces were surveyed for the relative incidence of WMV in 2004-2005. A total of 757 symptomatic cucurbit and 31 weed species were collected and assayed for infection with WMV. Of 788 leaf samples from cucurbit and weed plants, 190 samples were positive by double antibody sandwich ELISA (DAS-ELISA) using specific polyclonal antibody. Among the weed species tested only colocynth (Citrullus colocynthis) was found to be infected with WMV. The coat protein (CP) gene from 18 representative isolates was PCR amplified, cloned, sequenced, and compared with the sequences available in GeneBank. Phylogenetic analysis using 778 nucleotide long sequences of the coat protein gene showed that these isolates fell into two; groups I and II. Only one isolates (KER.JI.1) was classified in the group II. This isolate had a wider host range and infected Nicotiana debneyii and Datura metel. None of the other 17 isolates could infect these two species. Members of group I were divided into three subgroups; A, B, and C. The subgroup I(B) appears to be a new subgroup comprising only of the Iranian isolates. Phylogenetic analysis based on 200 nucleotides coding for the N-terminal segment of the CP showed that all Iranian isolates except KER.JI.1 clustered with the previously reported WMV strains. All Iranian isolates had a DAG amino acid triplet which is involved in aphid transmissibility. This is the first report on sequence analysis of the nearly full-length CP cDNA clones of WMV isolates from Iran.

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“…In New Zealand, SqMV was isolated from seeds imported from the United States but the virus could not be detected in the field (THOMAS 1973). Similarly, SqMV has been introduced into Iran with imported melon seed (IZADPANAH 1983) but earlier surveys of cucurbit viruses in the Iranian highlands have given no indication of field occurrence of this virus (EBRAHIM-NESBAT 1974, IZADPANAH 1970, RAHIMIAN and IZADPANAH 1978. However, the first survey of cucurbit viruses in the Bushehr Province indicated widespread occurrence of a veinbanding mosaic in melons caused by a virus different from previously reported viruses in this country (IZADPANAH 1986).…”

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confidence: 99%

Squash Mosaic Virus as the Cause of Melon Veinbanding Mosaic in Iran¹)

Izadpanah

1987

Journal of Phytopathology

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Melon veinbanding mosaic, a prevalent disease in the Bushehr Province of Iran, is characterized by formation of green veinbanding in varieties of Cucumis melo L. The disease is caused by an isometric virus of about 30 nm diameter. The virus was purified by acid and neutral pH clarification, polyethylene glycol precipitation and differential and density‐gradient centrifugation. The virus formed three zones in the density‐gradient columns. It reacted with a squash mosaic virus (SqMV) antiserumobtained from the United States in agar‐gel diffusion. Likewise, a U.S. isolate of SqMV reacted with the antiserum prepared against the Iranian isolate. The virus was seed transmissible in snakemelon. This is the first report on the field occurrence ofSqMV in Iran. The Iranian isolate appears to belong to the serotype 1 of SqMV.

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Identity and Prevalence of Mosaic-inducing Cucurbit Viruses in Shiraz, Iran)

Cited by 13 publications

References 12 publications

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran

Squash Mosaic Virus as the Cause of Melon Veinbanding Mosaic in Iran¹)

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Identity and Prevalence of Mosaic-inducing Cucurbit Viruses in Shiraz, Iran)

Cited by 13 publications

References 12 publications

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Characterization of a Watermelon mosaic virus Isolate Inducing a Severe Disease in Watermelon in Saudi Arabia

Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran

Squash Mosaic Virus as the Cause of Melon Veinbanding Mosaic in Iran1)

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Squash Mosaic Virus as the Cause of Melon Veinbanding Mosaic in Iran¹)