Identifying the function of genes involved in excreted vesicle formation in Acanthamoeba castellanii containing Legionella pneumophila

Abstract

Background Legionella spp. can survive and replicate inside host cells such as protozoa and macrophages. After enough growth, Legionella is released from the host cells as free legionellae or Legionella-filled vesicles. The vesicles support Legionella to survive for a long time in the environment and transmit to a new host. In this study, we identified the differentially expressed genes of Acanthamoeba infected by Legionella (ACA1_114460, ACA1_091500, and ACA1_362260) and examined their roles in the formation of the excreted vesicles and escape of Legionella from the Acanthamoeba. Methods After ingestion of Escherichia coli and Legionella pneumophila, expression levels of target genes in Acanthamoeba were measured by real-time polymerase chain reaction (PCR) analysis. The roles of target genes were investigated by transfection of small interfering RNA (siRNA). The formation of Legionella-containing excreted vesicles and the vesicular co-localization with the lysosomes were examined by Giemsa stain and LysoTracker stain. Results ACA1_114460, ACA1_091500, and ACA1_362260 were upregulated after ingestion of Legionella in Acanthamoeba. ACA1_114460- and ACA1_091500-silenced Acanthamoeba failed to form the Legionella-containing excreted vesicles. Legionella was released as free legionellae from the Acanthamoeba. When the ACA1_362260 of Acanthamoeba was silenced, Legionella-containing excreted vesicles were fused with the lysosome. Conclusions These results indicated that ACA1_114460, ACA1_091500, and ACA1_362260 of Acanthamoeba played important roles in the formation of Legionella-containing excreted vesicles and inhibition of the lysosomal co-localization with the phagosome. Graphical Abstract

Phagocytosis-associated genes in Acanthamoeba castellanii feeding on Escherichia coli

“…Based on the findings of this previous study, it was hypothesized that the genes upregulated in E. coli -ingested Acanthamoeba were involved in the phagocytosis of Acanthamoeba and those upregulated in L. pneumophila -ingested Acanthamoeba were involved in promoting its survival and endosymbiosis within Acanthamoeba . Among the upregulated genes in L. pneumophila -ingested A. castellanii , ACA1_114460 , ACA1_091500 , and ACA1_362260 were found to be integral to the formation of excretory vesicles containing L. pneumophila along with the lysosomal colocalization with the vesicles [ 10 ].…”