Identifying the differentially expressed microRNAs in esophagus squamous cell carcinoma of Kazakh patients in Xinjiang

Abstract

Despite improvements in diagnosis and treatment, the survival of patients with advanced stages of esophageal squamous cell carcinoma (ESCC) remains poor. Therefore, novel biomarkers that can assist with early detection of ESCC are required. In the present study, three paired ESCC and normal esophageal tissue samples from Xinjiang Kazakh patients were obtained and microRNA (miRNA) microarray analysis was used to detect the differentially-expressed miRNAs. The target genes of the identified miRNAs were predicted using miRWalk software. A total of 23 miRNAs were differently expressed in Kazakh patients with ESCC. Gene Ontology enrichment analysis demonstrated that the upregulated miRNAs were predominantly associated with the ‘vesicle’ and ‘membrane-bounded vesicle’ terms, while the downregulated miRNAs were primarily associated with the term ‘negative regulation of integrin-mediated signaling pathway’. The most highly enriched Kyoto Encyclopedia of Genes and Genomes pathway for the differentially-expressed miRNAs was ‘Endocrine and other factor-regulated calcium reabsorption’. Protein-protein interaction network analysis revealed that IQ motif containing GTPase activating protein 1, RAB11A, lysine acetyltransferase 2B, catenin α 1 and tight junction protein 2 were hub genes of the network. In conclusion, a number of differentially-expressed miRNAs were identified in ESCC tissues samples from Xinjiang Kazakh patients, which may improve the understanding of the processes of tumorigenesis and development.

The miR-1224-5p/TNS4/EGFR axis inhibits tumour progression in oesophageal squamous cell carcinoma

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Survival estimation in patients with stomach and esophageal carcinoma using miRNA expression profiles

“…A quantitative real-time PCR analysis reported that hsa-miR-760 is significantly downregulated in ESCA tissues and cell lines, suggesting that this miRNA could be used as a prognostic indicator [57] . Significant differential expression of hsa-miR-769-5p was observed in ESCA tissue when compared to the normal tissues [58] . Over-expression of hsa-miR-1301-3p induces cell proliferation and tumorigenesis in gastric cancer tissues [59] .…”

Integrated Bioinformatics Analysis of mRNAs and miRNAs Identified Potential Biomarkers of Oral Squamous Cell Carcinoma

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