Identifying the anti-metastasis effect of Anhydroicaritin on breast cancer: Coupling network pharmacology with experimental validation

Shi, Youyang; Wu, Yuanyuan; Li, Feifei; Zhang, Yang; Hua, Ciyi; Yang, Jianfeng; Zheng, Jinzhou; Chen, Lili; Wei, Zheng; Yue, Haiyan; Sun, Chenping; Zhou, Xiqiu; Liu, Sheng

doi:10.1016/j.jep.2022.115326

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…55 Anhydroicaritin, a natural component originated from Epimedii Herba, was shown to inhibit cancer cell multiplication, cell differentiation, invasion, and metastasis. 56 In addition, Anhydroicaritin can regulate the LKB1/AMPK/mTOR pathway to ameliorate diet-induced obesity and alleviate insulin resistance. 57 β-sitosterol is a primary component from common plants and vegetables that exerts antihyperlipidemia, anti-inflammation and anti-tumor effects.…”

Section: Discussionmentioning

confidence: 99%

Evidence Construction of Chuankezhi Injection Against Chronic Obstructive Pulmonary Disease: A Systematic Review and Network Pharmacology

Wei,

Zhong,

et al. 2024

COPD

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Chronic obstructive pulmonary disease (COPD) is a chronic respiratory disease with high prevalence, morbidity, and mortality. Chuankezhi (CKZ) injection, a Chinese patent medicine, has been commonly used for treating COPD. This study evaluated the clinical efficacy of CKZ injections in COPD patients and explored potential underlying mechanisms by integrating meta-analysis and network pharmacology. Research Methods: Randomized controlled trials (RCTs) were search in database by Web of Science, Cochrane Library and PubMed as of November 2022 for literature collection, and the Review Manager 5.4 was used to analyze the data. Through the network pharmacology method, the chemical components and their targets, as well as the disease targets were further analyzed. Results: A total of 15 RCTs including 1212 patients were included. The results of meta-analysis showed that CKZ injection can significantly improve the clinical effective rate (RR = 1.25, 95% CI: 1.14 to 1.36), and the clinical advantage was that it can significantly reduced acute exacerbation rate (RR = 0.29, 95% CI: 0.12 to 0.70) and COPD assessment test (CAT) scores (MD =−4.62, 95% CI:-8.966 to-0.28). A total of 31 chemical compounds and 178 potential targets for CKZ injection were obtained from the online databases. Molecular docking revealed that most key components and targets could form stable structure. Conclusion: This systematic review with meta-analysis and network pharmacology demonstrates that CKZ could effectively improve the clinical efficacy and safety in the treatment of COPD. Such efficacy may be related to an anti-inflammatory effect and immunoregulation of CKZ via multiple components, multiple targets and multiple pathways.

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Section: Discussionmentioning

confidence: 99%

Evidence Construction of Chuankezhi Injection Against Chronic Obstructive Pulmonary Disease: A Systematic Review and Network Pharmacology

Wei,

Zhong,

et al. 2024

COPD

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“…They are used for the treatment of osteoporosis, reproductive system disorders, kidney yang deficiency symptoms, and other diseases. With the increasing research on Epimedium and its ingredients, medical scientists have also found that they have the potential to treat uveitis [ 15 ], cardiovascular disease [ 16 ], tumors [ 17 , 18 ], influenza A [ 19 ], and neuroprotection [ 20 , 21 ] through anti-inflammation [ 22 ], antioxidant [ 20 ], regulating cell proliferation, apoptosis and autophagy [ 16 , 23 ], modulating immunity [ 24 ], and antiviral [ 19 ] mechanisms.…”

Section: Epimedium and Its Main Active Ingredientsmentioning

confidence: 99%

Effects of genus Epimedium in the treatment of osteoarthritis and relevant signaling pathways

Tong,

Wang,

Dong

et al. 2023

Chin Med

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Osteoarthritis (OA) is a common chronic degenerative joint disease in clinical practice with a high prevalence, especially in the elderly. Traditional Chinese Medicine (TCM) believes that OA belongs to the category of “Bi syndrome” and the “bone Bi syndrome”. The etiology and pathogenesis lie in the deficiency of the liver and kidney, the deficiency of Qi and blood, and external exposure to wind, cold, and dampness. Epimedium is a yang-reinforcing herb in TCM, which can tonify the liver and kidney, strengthen muscles and bones, dispel wind, cold and dampness, and can treat both the symptoms and the root cause of “bone Bi syndrome”. In addition, Epimedium contains a large number of ingredients. Through modern science and technology, more than 270 compounds have been found in Epimedium, among which flavonoids are the main active ingredients. Therefore, our study will review the effects and mechanisms of genus Epimedium in treating OA from two aspects: (1) Introduction of Epimedium and its main active ingredients; (2) Effects of Epimedium and its active ingredients in treating OA and relevant signaling pathways, in order to provide more ideas for OA treatment.

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“…A previous study revealed that AHI suppressed EMT by increasing glutathione peroxidase 1 in breast cancer cell [ 19 ]. In addition, AHI was reported to play an anti-breast cancer role by inhibiting cancer cell proliferation and metastasis [ 20 ]. AHI plays a promising role in breast cancer, however, studies on the application of AHI in HCC treatment remain limited, especially at the molecular mechanism.…”

Section: Introductionmentioning

confidence: 99%

Anhydroicaritin suppresses tumor progression via the PI3K/AKT signaling pathway in hepatocellular carcinoma

Wang

Chen

Cui

et al. 2023

Aging

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Background: Hepatocellular carcinoma (HCC) is one of the most malignant tumors. The in vitro experiments on the application of Anhydroicaritin (AHI), the active ingredient of Bushen Huayu Decoction, in HCC treatment remain limited, particularly regarding its molecular mechanism. Methods: The TCMSP platform was used for drug ingredient screening. The GeneCards database and DisGeNET database are used to collect liver cancer targets. PPI network construction of active component-target intersection target was completed with string database. The GO and KEGG pathway analyses were performed via bioinformatics analysis. The molecular docking was used to confirm AHI’s target proteins. The in vitro experiments were performed to validate the effect of AHI on HCC cell and explore the molecular mechanism by western blotting analysis. Results: Through the intersection, 155 intersection targets are finally obtained. The top 15 active ingredients were quercetin, kaempferol, beta-sitosterol, luteolin, beta-carotene, Stigmasterol, naringenin, formononetin, baicalein, Anhydroicaritin, isorhamnetin, licochalcone, 7-O-methylisomucronulatol, aloe-emodin and 8-O-Methylreyusi. The molecular mocking analysis showed that the four active components (quercetin, kaempferol, luteolin and AHI) and targets had a good binding activity (affinity ≤ 5 kcal/mol). In vitro experiments reveled that AHI could suppress tumor proliferation, invasion and metastasis of HCC cells. Further analysis showed that AHI inhibited tumor growth by PI3K/AKT signal pathway in HCC. Conclusions: The Bushen Huayu Decoction and its active ingredient AHI could fight HCC. The potential mechanism may be associated with inhibiting the activation of PI3K/AKT signal pathway, which may serve as a potential treatment for HCC therapy.

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Identifying the anti-metastasis effect of Anhydroicaritin on breast cancer: Coupling network pharmacology with experimental validation

Cited by 10 publications

References 12 publications

Evidence Construction of Chuankezhi Injection Against Chronic Obstructive Pulmonary Disease: A Systematic Review and Network Pharmacology

Evidence Construction of Chuankezhi Injection Against Chronic Obstructive Pulmonary Disease: A Systematic Review and Network Pharmacology

Effects of genus Epimedium in the treatment of osteoarthritis and relevant signaling pathways

Anhydroicaritin suppresses tumor progression via the PI3K/AKT signaling pathway in hepatocellular carcinoma

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