Identifying single-strain growth patterns of human gut microbes in response to preterm human milk and formula

Engevik, Melinda A.; Stripe, Leah K.; Baatz, John E.; Wagner, Carol L.; Chetta, Katherine E.

doi:10.1039/d2fo00447j

Cited by 4 publications

(5 citation statements)

References 108 publications

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“…The advantage of using a chemically defined medium is that the supernatant is amenable to downstream LC-MS/MS analysis since it does not contain components such as peptone and yeast extract. We have used this defined medium to examine the growth of diverse microbes and measure bacterial metabolites and proteins by LC-MS/MS [ 19 , 20 , 21 , 22 , 23 ]. However, this medium has not been validated for antibiotic studies, and the current gold standard medium is Mueller Hinton broth.…”

Section: Discussionmentioning

confidence: 99%

Profiling Antibiotic Resistance in Acinetobacter calcoaceticus

2022

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Background: Acinetobacter spp. have emerged as troublesome pathogens due to their multi-drug resistance. The majority of the work to date has focused on the antibiotic resistance profile of Acinetobacter baumannii. Although A. calcoaceticus strains are isolated in the hospital setting, limited information is available on these closely related species. Methods & Results: The computational analysis of antibiotic resistance genes in 1441 Acinetobacter genomes revealed that A. calcoaceticus harbored a similar repertoire of multi-drug efflux pump and beta-lactam resistance genes as A. baumannii, leading us to speculate that A. calcoaceticus would have a similar antibiotic resistance profile to A. baumannii. To profile the resistance patterns of A. calcoaceticus, strains were examined by Kirby–Bauer disk diffusion and phenotypic microarrays. We found that Acinetobacter strains were moderately to highly resistant to certain antibiotics within fluoroquinolones, aminoglycosides, tetracyclines, and other antibiotic classes. These data indicate that A. calcoaceticus has a similar antibiotic resistance profile as A. baumannii ATCC 19606. We also identified that all Acinetobacter species were sensitive to 5-fluoroorotic acid, novobiocin, and benzethonium chloride. Conclusion: Collectively, these data provide new insights into the antibiotic resistance in A. calcoaceticus and identify several antibiotics that could be beneficial in treating Acinetobacter infections.

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Section: Discussionmentioning

confidence: 99%

Profiling Antibiotic Resistance in Acinetobacter calcoaceticus

2022

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“…The maternal gastrointestinal microbiota is transferred to a newborn infant at birth. However, preterm infants have a generally more dysbiotic microbiome (30). The gut microbiota in preterm infants is characterized by limited microbial diversity and delayed colonization (30)(31)(32).…”

Section: Microbial Dysbiosis In Allergy and Necmentioning

confidence: 99%

“…However, preterm infants have a generally more dysbiotic microbiome (30). The gut microbiota in preterm infants is characterized by limited microbial diversity and delayed colonization (30)(31)(32). Preterm infants have increased Enterococci, Staphylococci, and Enterobacteriaceae (Enterobacter, Escherichia, and Klebsiella spp) and have less diversity of microbial constituents when compared to term infants (30,31,(33)(34)(35)(36)(37)(38)(39).…”

Section: Microbial Dysbiosis In Allergy and Necmentioning

confidence: 99%

“…The gut microbiota in preterm infants is characterized by limited microbial diversity and delayed colonization (30)(31)(32). Preterm infants have increased Enterococci, Staphylococci, and Enterobacteriaceae (Enterobacter, Escherichia, and Klebsiella spp) and have less diversity of microbial constituents when compared to term infants (30,31,(33)(34)(35)(36)(37)(38)(39). During vaginal delivery, the mother's microbiota is the main source of microorganisms colonizing newborns (29).…”

Section: Microbial Dysbiosis In Allergy and Necmentioning

confidence: 99%

“…In breastfed newborns, Bifidobacterium appear as early as day 2 of life and by the second week is the predominant bacterial genus in the GI tract. Infants fed formula have increased Escherichia, Clostridia, Bacteroides, and Enterobacteriaceae (30,(40)(41)(42)(43)(44)(45)(46)(47). These predominate until solid foods are introduced and infants are weaned from breast milk.…”

Section: Microbial Dysbiosis In Allergy and Necmentioning

confidence: 99%

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Exploring the links between necrotizing enterocolitis and cow's milk protein allergy in preterm infants: a narrative review

Moak,

Boone,

Eidson

et al. 2023

Front. Pediatr.

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A broad range of allergic disorders and intolerance are associated with cow's milk protein in the infant diet. Allergy and intolerance to cow's milk proteins are commonly recognized in the healthy term infant, and the prevalence cow's milk protein intolerance (CMPI) varies widely but 5 challenge confirmed studies free from selection bias ranged from 1.9%-4.9%. These disorders are classified by the presence of IgE, non-IgE or T-cell-mediated signaling. Additionally, the severity of these adverse food reactions can range from mild gastrointestinal symptoms to severe sepsis-like episodes, as in the case of food protein-induced enterocolitis syndrome (FPIES). Food protein-induced intolerance in the healthy young infant lies in stark contrast to enterocolitis that typically occurs in the preterm neonate. Necrotizing enterocolitis (NEC) is a distinct progressive disease process, usually characterized by a high mortality rate, with a risk of death from 30% to 50%. While its exact etiology is unclear, its main triggers include formula (cow's milk protein), hypoxia, perfusion-related issues, and unregulated inflammation in the premature intestine. The distinction between NEC and cow's milk protein intolerance is difficult to discern in some cases. In the late preterm population, infants with colitis can have both NEC and cow's milk intolerance on the differential. In infants with multiple episodes of mild NEC, cow's milk protein intolerance may be the underlying diagnosis. In this review, we compare the pathophysiological characteristics, diagnosis and treatment of disorders of cow's milk protein intolerance with the entity of preterm NEC. This review highlights similarities in both entities and may inspire future cross-disciplinary research.

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Linking human milk oligosaccharide metabolism and early life gut microbiota: bifidobacteria and beyond

Lordan,

Roche,

Delsing

et al. 2024

Microbiol Mol Biol Rev

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SUMMARY Human milk oligosaccharides (HMOs) are complex, multi-functional glycans present in human breast milk. They represent an intricate mix of heterogeneous structures which reach the infant intestine in an intact form as they resist gastrointestinal digestion. Therefore, they confer a multitude of benefits, directly and/or indirectly, to the developing neonate. Certain bifidobacterial species, being among the earliest gut colonizers of breast-fed infants, have an adapted functional capacity to metabolize various HMO structures. This ability is typically observed in infant-associated bifidobacteria, as opposed to bifidobacteria associated with a mature microbiota. In recent years, information has been gleaned regarding how these infant-associated bifidobacteria as well as certain other taxa are able to assimilate HMOs, including the mechanistic strategies enabling their acquisition and consumption. Additionally, complex metabolic interactions occur between microbes facilitated by HMOs, including the utilization of breakdown products released from HMO degradation. Interest in HMO-mediated changes in microbial composition and function has been the focal point of numerous studies, in recent times fueled by the availability of individual biosynthetic HMOs, some of which are now commonly included in infant formula. In this review, we outline the main HMO assimilatory and catabolic strategies employed by infant-associated bifidobacteria, discuss other taxa that exhibit breast milk glycan degradation capacity, and cover HMO-supported cross-feeding interactions and related metabolites that have been described thus far.

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Identifying single-strain growth patterns of human gut microbes in response to preterm human milk and formula

Abstract: The intestinal microbiota of the preterm neonate has become a major research focus, with evidence emerging that the microbiota influences both short and long-term health outcomes, in the neonatal intensive...

Cited by 4 publications

References 108 publications

Profiling Antibiotic Resistance in Acinetobacter calcoaceticus

Profiling Antibiotic Resistance in Acinetobacter calcoaceticus

Exploring the links between necrotizing enterocolitis and cow's milk protein allergy in preterm infants: a narrative review

Linking human milk oligosaccharide metabolism and early life gut microbiota: bifidobacteria and beyond

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