Identifying Ni-complexed natural organic matters in a relatively stagnant river water

Abstract

A B S T R AC TPretreatment protocols, using preparative liquid chromatography (prep-LC), and high resolution mass spectrometer methods, were developed to identify potential Ni-natural organic matter (NOM) complexation, for a relatively stagnant river water sample. The former and the latter were performed, using a large separation column, packed with C18 and size exclusion resin media, and ion trap-time of fl ight (IT-TOF) mass spectrometer, respectively. The NOM samples were effectively fractionated into four different peaks, with helps of both RI and UV detections, and further subjected to mass analyses using the IT-TOF, which provided distinct m/z peaks pairs in mass spectra, with m/z peaks difference of ca. 58, as evidence of Ni-complexed NOM.